PubMed:3663601 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/3663601","sourcedb":"PubMed","sourceid":"3663601","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/3663601","text":"Distinguishing among protein kinases by substrate specificities.\nIn the previous paper, N-methylated peptides were shown to be sensitive probes of substrate conformation within the adenosine cyclic 3',5'-phosphate dependent protein kinase (A-kinase) active site. While it has been shown that other protein kinases will catalyze the phosphorylation of the same peptide sequences as A-kinase, there is as yet little information as to whether the protein kinases differentiate between substrates on the basis of conformation. For this reason, the conformationally restricted N-methylated peptides were used to probe the active site of guanosine cyclic 3',5'-phosphate dependent protein kinase (G-kinase), which is homologous in sequence to [Takio, K., Wade, R. D., Smith, S. B., Krebs, E. G., Walsh, K. A., \u0026 Titani, K. (1984) Biochemistry 23, 4207-4218] and which has substrate specificities similar to [Lincoln, T. M., \u0026 Corbin, J. D. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 3239-3243] those of A-kinase. Although this enzyme appears to bind the peptides in a conformation resembling that of conformation A, it is more able to accommodate backbone methylation than is A-kinase. A peptide substrate at least 700-fold selective for G-kinase over A-kinase was found. Backbone methylation may, therefore, represent a way of making peptide substrates and inhibitors selective for a particular kinase.","tracks":[{"project":"bionlp-st-epi-2011-training","denotations":[{"id":"T1","span":{"begin":632,"end":689},"obj":"Protein"},{"id":"T2","span":{"begin":691,"end":699},"obj":"Protein"},{"id":"T3","span":{"begin":1230,"end":1238},"obj":"Protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T2","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T3","pred":"source","obj":"bionlp-st-epi-2011-training"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"bionlp-st-epi-2011-training","color":"#93ecc9","default":true}]}]}}