PubMed:35811008 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/35811008","sourcedb":"PubMed","sourceid":"35811008","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/35811008","text":"Quantitative label-free proteomic analysis of human follicle fluid to identify novel candidate protein biomarker for endometriosis-associated infertility.\nBACKGROUND: Endometriosis (EM) leads to a decline in fertility, which is characterized by a decrease in the number and quality of follicles, and thus has a negative impact on in vitro fertilization (IVF) outcomes. However, the mechanism of how EM affects oocytes and leads to infertility remains unclear. As a potentially available sample directly related to oocyte growth, follicular fluid (FF) has important research value. Evaluating the association of FF content and EM-associated infertility through proteomics may helpful to explore the possible pathogenesis of EM-associated infertility.\nMETHODS: In the present experimental study, from August 2019 to June 2020, FF samples were obtained as control group (CON-G; n = 10) from women with no one female factor of infertility and were undergoing IVF due to other reasons, 20 women with EM-associated infertility undergoing IVF with no other female factors were distributed into the EM group according to the time for IVF: (i) EM-group 1 (EM-G1, Stage I to Stage III, n = 10); (ii) EM-group 2 (EM-G2, Stage I to Stage III, n = 10). label-free quantitative proteomics (LFQP) technology and parallel reaction monitoring (PRM) approach were combined to aid in identifying and validating FF protein biomarkers for EM-associated infertility. In PRM analysis, another 20 subjects were enrolled as EM-associated infertility group (EM,Stage I to Stage III, n = 10) and controls (CON, n = 10) within the same time and inclusion criteria are the same as previously described. Finally, a potential protein biomarker panel of FF differential expressed proteins to EM-associated infertility was also evaluated by t-test and receiver operating characteristic (ROC) curve and binary Logistic regression models.\nRESULTS: 7 significant differential expressed proteins which closely related to EM-associated infertility were found by LFQP technology, among which immunoglobulin lambda variable 7-46 (IGLV7-46), Immunoglobulin heavy constant gamma 2 (IGHG2), glia-derived nexin (GDN) and Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) were significantly up-regulated (p \u003c 0.05), while corticosteroid-binding globulin (CBG), angiotensinogen (AGT) and Fetuin-B (FETUB) were significantly down regulated (p \u003c 0.05). Additionally, GDN and AGT was identified as a potential protein biomarker by further PRM analysis for EM-associated infertility according to ROC curve analysis and t-test (p \u003c 0.05), the area under the curve (AUC) for GDN and AGT was 0.78 and 0.69 with optimum sensitivity of 50%, 70% and specificity of 100%, 90%, respectively. According to binary logistic regression and evaluated ROC analysis, the AUC for the combination of GDN and AGT was 0.80.\nCONCLUSIONS: To the best of our knowledge, this is the first time that elevated GDN protein levels have been found in the FF of patients with EM-associated infertility. Combining LFQP technology and PRM method we found the abnormal of GDN and AGT in FF may be the potential cause of EM-associated infertility which may help to better understand the physiological and pathological mechanism of EM-associated infertility. Further experimental studies are required to confirm their mechanism in EM-associated infertility. The results of this study are also consistent with the previous conclusion that EM is a chronic inflammatory disease.\nSIGNIFICANCE: To the best of our knowledge, this is the first time that elevated GDN protein levels have been found in the follicular fluid of patients with EM-associated infertility. Combining LFQP technology and PRM methods we found the abnormal of GDN and AGT protein in FF may be the potential cause of EM-associated infertility which may help to better understand the physiological and pathological mechanism of EM-associated infertility. Clinically, it has been recognized that EM is related to infertility, but the mechanism remains unclear. Our study combines label-free quantitative proteomics technology and parallel reaction monitoring methods to identify and verify the FF protein biomarkers of EM-associated infertility, which provides a good research method for follow-up research.","tracks":[{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":117,"end":130},"obj":"Phenotype"},{"id":"T2","span":{"begin":142,"end":153},"obj":"Phenotype"},{"id":"T3","span":{"begin":167,"end":180},"obj":"Phenotype"},{"id":"T4","span":{"begin":182,"end":184},"obj":"Phenotype"},{"id":"T5","span":{"begin":399,"end":401},"obj":"Phenotype"},{"id":"T6","span":{"begin":431,"end":442},"obj":"Phenotype"},{"id":"T7","span":{"begin":626,"end":628},"obj":"Phenotype"},{"id":"T8","span":{"begin":640,"end":651},"obj":"Phenotype"},{"id":"T9","span":{"begin":723,"end":725},"obj":"Phenotype"},{"id":"T10","span":{"begin":737,"end":748},"obj":"Phenotype"},{"id":"T11","span":{"begin":923,"end":934},"obj":"Phenotype"},{"id":"T12","span":{"begin":995,"end":997},"obj":"Phenotype"},{"id":"T13","span":{"begin":1009,"end":1020},"obj":"Phenotype"},{"id":"T14","span":{"begin":1091,"end":1093},"obj":"Phenotype"},{"id":"T15","span":{"begin":1135,"end":1137},"obj":"Phenotype"},{"id":"T16","span":{"begin":1147,"end":1149},"obj":"Phenotype"},{"id":"T17","span":{"begin":1190,"end":1192},"obj":"Phenotype"},{"id":"T18","span":{"begin":1202,"end":1204},"obj":"Phenotype"},{"id":"T19","span":{"begin":1418,"end":1420},"obj":"Phenotype"},{"id":"T20","span":{"begin":1432,"end":1443},"obj":"Phenotype"},{"id":"T21","span":{"begin":1499,"end":1501},"obj":"Phenotype"},{"id":"T22","span":{"begin":1513,"end":1524},"obj":"Phenotype"},{"id":"T23","span":{"begin":1532,"end":1534},"obj":"Phenotype"},{"id":"T24","span":{"begin":1760,"end":1762},"obj":"Phenotype"},{"id":"T25","span":{"begin":1774,"end":1785},"obj":"Phenotype"},{"id":"T26","span":{"begin":1984,"end":1986},"obj":"Phenotype"},{"id":"T27","span":{"begin":1998,"end":2009},"obj":"Phenotype"},{"id":"T28","span":{"begin":2510,"end":2512},"obj":"Phenotype"},{"id":"T29","span":{"begin":2524,"end":2535},"obj":"Phenotype"},{"id":"T30","span":{"begin":3000,"end":3002},"obj":"Phenotype"},{"id":"T31","span":{"begin":3014,"end":3025},"obj":"Phenotype"},{"id":"T32","span":{"begin":3141,"end":3143},"obj":"Phenotype"},{"id":"T33","span":{"begin":3155,"end":3166},"obj":"Phenotype"},{"id":"T34","span":{"begin":3251,"end":3253},"obj":"Phenotype"},{"id":"T35","span":{"begin":3265,"end":3276},"obj":"Phenotype"},{"id":"T36","span":{"begin":3350,"end":3352},"obj":"Phenotype"},{"id":"T37","span":{"begin":3364,"end":3375},"obj":"Phenotype"},{"id":"T38","span":{"begin":3457,"end":3459},"obj":"Phenotype"},{"id":"T39","span":{"begin":3652,"end":3654},"obj":"Phenotype"},{"id":"T40","span":{"begin":3666,"end":3677},"obj":"Phenotype"},{"id":"T41","span":{"begin":3802,"end":3804},"obj":"Phenotype"},{"id":"T42","span":{"begin":3816,"end":3827},"obj":"Phenotype"},{"id":"T43","span":{"begin":3912,"end":3914},"obj":"Phenotype"},{"id":"T44","span":{"begin":3926,"end":3937},"obj":"Phenotype"},{"id":"T45","span":{"begin":3979,"end":3981},"obj":"Phenotype"},{"id":"T46","span":{"begin":3996,"end":4007},"obj":"Phenotype"},{"id":"T47","span":{"begin":4202,"end":4204},"obj":"Phenotype"},{"id":"T48","span":{"begin":4216,"end":4227},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0030127"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0000789"},{"id":"A3","pred":"hp_id","subj":"T3","obj":"HP:0030127"},{"id":"A4","pred":"hp_id","subj":"T4","obj":"HP:0030127"},{"id":"A5","pred":"hp_id","subj":"T5","obj":"HP:0030127"},{"id":"A6","pred":"hp_id","subj":"T6","obj":"HP:0000789"},{"id":"A7","pred":"hp_id","subj":"T7","obj":"HP:0030127"},{"id":"A8","pred":"hp_id","subj":"T8","obj":"HP:0000789"},{"id":"A9","pred":"hp_id","subj":"T9","obj":"HP:0030127"},{"id":"A10","pred":"hp_id","subj":"T10","obj":"HP:0000789"},{"id":"A11","pred":"hp_id","subj":"T11","obj":"HP:0000789"},{"id":"A12","pred":"hp_id","subj":"T12","obj":"HP:0030127"},{"id":"A13","pred":"hp_id","subj":"T13","obj":"HP:0000789"},{"id":"A14","pred":"hp_id","subj":"T14","obj":"HP:0030127"},{"id":"A15","pred":"hp_id","subj":"T15","obj":"HP:0030127"},{"id":"A16","pred":"hp_id","subj":"T16","obj":"HP:0030127"},{"id":"A17","pred":"hp_id","subj":"T17","obj":"HP:0030127"},{"id":"A18","pred":"hp_id","subj":"T18","obj":"HP:0030127"},{"id":"A19","pred":"hp_id","subj":"T19","obj":"HP:0030127"},{"id":"A20","pred":"hp_id","subj":"T20","obj":"HP:0000789"},{"id":"A21","pred":"hp_id","subj":"T21","obj":"HP:0030127"},{"id":"A22","pred":"hp_id","subj":"T22","obj":"HP:0000789"},{"id":"A23","pred":"hp_id","subj":"T23","obj":"HP:0030127"},{"id":"A24","pred":"hp_id","subj":"T24","obj":"HP:0030127"},{"id":"A25","pred":"hp_id","subj":"T25","obj":"HP:0000789"},{"id":"A26","pred":"hp_id","subj":"T26","obj":"HP:0030127"},{"id":"A27","pred":"hp_id","subj":"T27","obj":"HP:0000789"},{"id":"A28","pred":"hp_id","subj":"T28","obj":"HP:0030127"},{"id":"A29","pred":"hp_id","subj":"T29","obj":"HP:0000789"},{"id":"A30","pred":"hp_id","subj":"T30","obj":"HP:0030127"},{"id":"A31","pred":"hp_id","subj":"T31","obj":"HP:0000789"},{"id":"A32","pred":"hp_id","subj":"T32","obj":"HP:0030127"},{"id":"A33","pred":"hp_id","subj":"T33","obj":"HP:0000789"},{"id":"A34","pred":"hp_id","subj":"T34","obj":"HP:0030127"},{"id":"A35","pred":"hp_id","subj":"T35","obj":"HP:0000789"},{"id":"A36","pred":"hp_id","subj":"T36","obj":"HP:0030127"},{"id":"A37","pred":"hp_id","subj":"T37","obj":"HP:0000789"},{"id":"A38","pred":"hp_id","subj":"T38","obj":"HP:0030127"},{"id":"A39","pred":"hp_id","subj":"T39","obj":"HP:0030127"},{"id":"A40","pred":"hp_id","subj":"T40","obj":"HP:0000789"},{"id":"A41","pred":"hp_id","subj":"T41","obj":"HP:0030127"},{"id":"A42","pred":"hp_id","subj":"T42","obj":"HP:0000789"},{"id":"A43","pred":"hp_id","subj":"T43","obj":"HP:0030127"},{"id":"A44","pred":"hp_id","subj":"T44","obj":"HP:0000789"},{"id":"A45","pred":"hp_id","subj":"T45","obj":"HP:0030127"},{"id":"A46","pred":"hp_id","subj":"T46","obj":"HP:0000789"},{"id":"A47","pred":"hp_id","subj":"T47","obj":"HP:0030127"},{"id":"A48","pred":"hp_id","subj":"T48","obj":"HP:0000789"},{"subj":"T1","pred":"source","obj":"HP-phenotype"},{"subj":"T2","pred":"source","obj":"HP-phenotype"},{"subj":"T3","pred":"source","obj":"HP-phenotype"},{"subj":"T4","pred":"source","obj":"HP-phenotype"},{"subj":"T5","pred":"source","obj":"HP-phenotype"},{"subj":"T6","pred":"source","obj":"HP-phenotype"},{"subj":"T7","pred":"source","obj":"HP-phenotype"},{"subj":"T8","pred":"source","obj":"HP-phenotype"},{"subj":"T9","pred":"source","obj":"HP-phenotype"},{"subj":"T10","pred":"source","obj":"HP-phenotype"},{"subj":"T11","pred":"source","obj":"HP-phenotype"},{"subj":"T12","pred":"source","obj":"HP-phenotype"},{"subj":"T13","pred":"source","obj":"HP-phenotype"},{"subj":"T14","pred":"source","obj":"HP-phenotype"},{"subj":"T15","pred":"source","obj":"HP-phenotype"},{"subj":"T16","pred":"source","obj":"HP-phenotype"},{"subj":"T17","pred":"source","obj":"HP-phenotype"},{"subj":"T18","pred":"source","obj":"HP-phenotype"},{"subj":"T19","pred":"source","obj":"HP-phenotype"},{"subj":"T20","pred":"source","obj":"HP-phenotype"},{"subj":"T21","pred":"source","obj":"HP-phenotype"},{"subj":"T22","pred":"source","obj":"HP-phenotype"},{"subj":"T23","pred":"source","obj":"HP-phenotype"},{"subj":"T24","pred":"source","obj":"HP-phenotype"},{"subj":"T25","pred":"source","obj":"HP-phenotype"},{"subj":"T26","pred":"source","obj":"HP-phenotype"},{"subj":"T27","pred":"source","obj":"HP-phenotype"},{"subj":"T28","pred":"source","obj":"HP-phenotype"},{"subj":"T29","pred":"source","obj":"HP-phenotype"},{"subj":"T30","pred":"source","obj":"HP-phenotype"},{"subj":"T31","pred":"source","obj":"HP-phenotype"},{"subj":"T32","pred":"source","obj":"HP-phenotype"},{"subj":"T33","pred":"source","obj":"HP-phenotype"},{"subj":"T34","pred":"source","obj":"HP-phenotype"},{"subj":"T35","pred":"source","obj":"HP-phenotype"},{"subj":"T36","pred":"source","obj":"HP-phenotype"},{"subj":"T37","pred":"source","obj":"HP-phenotype"},{"subj":"T38","pred":"source","obj":"HP-phenotype"},{"subj":"T39","pred":"source","obj":"HP-phenotype"},{"subj":"T40","pred":"source","obj":"HP-phenotype"},{"subj":"T41","pred":"source","obj":"HP-phenotype"},{"subj":"T42","pred":"source","obj":"HP-phenotype"},{"subj":"T43","pred":"source","obj":"HP-phenotype"},{"subj":"T44","pred":"source","obj":"HP-phenotype"},{"subj":"T45","pred":"source","obj":"HP-phenotype"},{"subj":"T46","pred":"source","obj":"HP-phenotype"},{"subj":"T47","pred":"source","obj":"HP-phenotype"},{"subj":"T48","pred":"source","obj":"HP-phenotype"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":117,"end":130},"obj":"Disease"},{"id":"T2","span":{"begin":142,"end":153},"obj":"Disease"},{"id":"T3","span":{"begin":167,"end":180},"obj":"Disease"},{"id":"T4","span":{"begin":182,"end":184},"obj":"Disease"},{"id":"T5","span":{"begin":399,"end":401},"obj":"Disease"},{"id":"T6","span":{"begin":431,"end":442},"obj":"Disease"},{"id":"T7","span":{"begin":626,"end":628},"obj":"Disease"},{"id":"T8","span":{"begin":640,"end":651},"obj":"Disease"},{"id":"T9","span":{"begin":723,"end":725},"obj":"Disease"},{"id":"T10","span":{"begin":737,"end":748},"obj":"Disease"},{"id":"T11","span":{"begin":923,"end":934},"obj":"Disease"},{"id":"T12","span":{"begin":995,"end":997},"obj":"Disease"},{"id":"T13","span":{"begin":1009,"end":1020},"obj":"Disease"},{"id":"T14","span":{"begin":1091,"end":1093},"obj":"Disease"},{"id":"T15","span":{"begin":1135,"end":1137},"obj":"Disease"},{"id":"T16","span":{"begin":1147,"end":1149},"obj":"Disease"},{"id":"T17","span":{"begin":1190,"end":1192},"obj":"Disease"},{"id":"T18","span":{"begin":1202,"end":1204},"obj":"Disease"},{"id":"T19","span":{"begin":1418,"end":1420},"obj":"Disease"},{"id":"T20","span":{"begin":1432,"end":1443},"obj":"Disease"},{"id":"T21","span":{"begin":1499,"end":1501},"obj":"Disease"},{"id":"T22","span":{"begin":1513,"end":1524},"obj":"Disease"},{"id":"T23","span":{"begin":1532,"end":1534},"obj":"Disease"},{"id":"T24","span":{"begin":1760,"end":1762},"obj":"Disease"},{"id":"T25","span":{"begin":1774,"end":1785},"obj":"Disease"},{"id":"T26","span":{"begin":1984,"end":1986},"obj":"Disease"},{"id":"T27","span":{"begin":1998,"end":2009},"obj":"Disease"},{"id":"T28","span":{"begin":2510,"end":2512},"obj":"Disease"},{"id":"T29","span":{"begin":2524,"end":2535},"obj":"Disease"},{"id":"T30","span":{"begin":3000,"end":3002},"obj":"Disease"},{"id":"T31","span":{"begin":3014,"end":3025},"obj":"Disease"},{"id":"T32","span":{"begin":3141,"end":3143},"obj":"Disease"},{"id":"T33","span":{"begin":3155,"end":3166},"obj":"Disease"},{"id":"T34","span":{"begin":3251,"end":3253},"obj":"Disease"},{"id":"T35","span":{"begin":3265,"end":3276},"obj":"Disease"},{"id":"T36","span":{"begin":3350,"end":3352},"obj":"Disease"},{"id":"T37","span":{"begin":3364,"end":3375},"obj":"Disease"},{"id":"T38","span":{"begin":3457,"end":3459},"obj":"Disease"},{"id":"T39","span":{"begin":3473,"end":3493},"obj":"Disease"},{"id":"T40","span":{"begin":3652,"end":3654},"obj":"Disease"},{"id":"T41","span":{"begin":3666,"end":3677},"obj":"Disease"},{"id":"T42","span":{"begin":3802,"end":3804},"obj":"Disease"},{"id":"T43","span":{"begin":3816,"end":3827},"obj":"Disease"},{"id":"T44","span":{"begin":3912,"end":3914},"obj":"Disease"},{"id":"T45","span":{"begin":3926,"end":3937},"obj":"Disease"},{"id":"T46","span":{"begin":3979,"end":3981},"obj":"Disease"},{"id":"T47","span":{"begin":3996,"end":4007},"obj":"Disease"},{"id":"T48","span":{"begin":4202,"end":4204},"obj":"Disease"},{"id":"T49","span":{"begin":4216,"end":4227},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A8","pred":"mondo_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A10","pred":"mondo_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A11","pred":"mondo_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A12","pred":"mondo_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A13","pred":"mondo_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A14","pred":"mondo_id","subj":"T14","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A15","pred":"mondo_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A16","pred":"mondo_id","subj":"T16","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A17","pred":"mondo_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A18","pred":"mondo_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A19","pred":"mondo_id","subj":"T19","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A20","pred":"mondo_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A21","pred":"mondo_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A22","pred":"mondo_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A23","pred":"mondo_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A24","pred":"mondo_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A25","pred":"mondo_id","subj":"T25","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A26","pred":"mondo_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A27","pred":"mondo_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A28","pred":"mondo_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A29","pred":"mondo_id","subj":"T29","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A30","pred":"mondo_id","subj":"T30","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A31","pred":"mondo_id","subj":"T31","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A32","pred":"mondo_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A33","pred":"mondo_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A34","pred":"mondo_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A35","pred":"mondo_id","subj":"T35","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A36","pred":"mondo_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A37","pred":"mondo_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A38","pred":"mondo_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A39","pred":"mondo_id","subj":"T39","obj":"http://purl.obolibrary.org/obo/MONDO_0021166"},{"id":"A40","pred":"mondo_id","subj":"T40","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A41","pred":"mondo_id","subj":"T41","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A42","pred":"mondo_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A43","pred":"mondo_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A44","pred":"mondo_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A45","pred":"mondo_id","subj":"T45","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A46","pred":"mondo_id","subj":"T46","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A47","pred":"mondo_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A48","pred":"mondo_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/MONDO_0005133"},{"id":"A49","pred":"mondo_id","subj":"T49","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"},{"subj":"T3","pred":"source","obj":"mondo_disease"},{"subj":"T4","pred":"source","obj":"mondo_disease"},{"subj":"T5","pred":"source","obj":"mondo_disease"},{"subj":"T6","pred":"source","obj":"mondo_disease"},{"subj":"T7","pred":"source","obj":"mondo_disease"},{"subj":"T8","pred":"source","obj":"mondo_disease"},{"subj":"T9","pred":"source","obj":"mondo_disease"},{"subj":"T10","pred":"source","obj":"mondo_disease"},{"subj":"T11","pred":"source","obj":"mondo_disease"},{"subj":"T12","pred":"source","obj":"mondo_disease"},{"subj":"T13","pred":"source","obj":"mondo_disease"},{"subj":"T14","pred":"source","obj":"mondo_disease"},{"subj":"T15","pred":"source","obj":"mondo_disease"},{"subj":"T16","pred":"source","obj":"mondo_disease"},{"subj":"T17","pred":"source","obj":"mondo_disease"},{"subj":"T18","pred":"source","obj":"mondo_disease"},{"subj":"T19","pred":"source","obj":"mondo_disease"},{"subj":"T20","pred":"source","obj":"mondo_disease"},{"subj":"T21","pred":"source","obj":"mondo_disease"},{"subj":"T22","pred":"source","obj":"mondo_disease"},{"subj":"T23","pred":"source","obj":"mondo_disease"},{"subj":"T24","pred":"source","obj":"mondo_disease"},{"subj":"T25","pred":"source","obj":"mondo_disease"},{"subj":"T26","pred":"source","obj":"mondo_disease"},{"subj":"T27","pred":"source","obj":"mondo_disease"},{"subj":"T28","pred":"source","obj":"mondo_disease"},{"subj":"T29","pred":"source","obj":"mondo_disease"},{"subj":"T30","pred":"source","obj":"mondo_disease"},{"subj":"T31","pred":"source","obj":"mondo_disease"},{"subj":"T32","pred":"source","obj":"mondo_disease"},{"subj":"T33","pred":"source","obj":"mondo_disease"},{"subj":"T34","pred":"source","obj":"mondo_disease"},{"subj":"T35","pred":"source","obj":"mondo_disease"},{"subj":"T36","pred":"source","obj":"mondo_disease"},{"subj":"T37","pred":"source","obj":"mondo_disease"},{"subj":"T38","pred":"source","obj":"mondo_disease"},{"subj":"T39","pred":"source","obj":"mondo_disease"},{"subj":"T40","pred":"source","obj":"mondo_disease"},{"subj":"T41","pred":"source","obj":"mondo_disease"},{"subj":"T42","pred":"source","obj":"mondo_disease"},{"subj":"T43","pred":"source","obj":"mondo_disease"},{"subj":"T44","pred":"source","obj":"mondo_disease"},{"subj":"T45","pred":"source","obj":"mondo_disease"},{"subj":"T46","pred":"source","obj":"mondo_disease"},{"subj":"T47","pred":"source","obj":"mondo_disease"},{"subj":"T48","pred":"source","obj":"mondo_disease"},{"subj":"T49","pred":"source","obj":"mondo_disease"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":46,"end":51},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"subj":"T1","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":52,"end":60},"obj":"Body_part"},{"id":"T3","span":{"begin":61,"end":66},"obj":"Body_part"},{"id":"T4","span":{"begin":285,"end":294},"obj":"Body_part"},{"id":"T6","span":{"begin":514,"end":520},"obj":"Body_part"},{"id":"T7","span":{"begin":529,"end":545},"obj":"Body_part"},{"id":"T8","span":{"begin":547,"end":549},"obj":"Body_part"},{"id":"T9","span":{"begin":611,"end":613},"obj":"Body_part"},{"id":"T10","span":{"begin":825,"end":827},"obj":"Body_part"},{"id":"T11","span":{"begin":1392,"end":1394},"obj":"Body_part"},{"id":"T12","span":{"begin":1722,"end":1724},"obj":"Body_part"},{"id":"T13","span":{"begin":2068,"end":2074},"obj":"Body_part"},{"id":"T14","span":{"begin":2980,"end":2982},"obj":"Body_part"},{"id":"T15","span":{"begin":3108,"end":3110},"obj":"Body_part"},{"id":"T16","span":{"begin":3618,"end":3634},"obj":"Body_part"},{"id":"T17","span":{"begin":3769,"end":3771},"obj":"Body_part"},{"id":"T18","span":{"begin":4177,"end":4179},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0003199"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0036150"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0006314"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0003199"},{"id":"A5","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0036150"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL_0000023"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A8","pred":"uberon_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A9","pred":"uberon_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A10","pred":"uberon_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A11","pred":"uberon_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A12","pred":"uberon_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A13","pred":"uberon_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/UBERON_0013424"},{"id":"A14","pred":"uberon_id","subj":"T14","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A15","pred":"uberon_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A16","pred":"uberon_id","subj":"T16","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A17","pred":"uberon_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"id":"A18","pred":"uberon_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/UBERON_0000038"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T4","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T6","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T7","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T8","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T9","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T10","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T11","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T12","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T13","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T14","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T15","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T16","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T17","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T18","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":410,"end":417},"obj":"Cell"},{"id":"T2","span":{"begin":514,"end":520},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000023"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000023"},{"subj":"T1","pred":"source","obj":"CL-cell"},{"subj":"T2","pred":"source","obj":"CL-cell"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"HP-phenotype","color":"#93ecad","default":true},{"id":"mondo_disease","color":"#ec9393"},{"id":"NCBITAXON","color":"#93acec"},{"id":"Anatomy-UBERON","color":"#c7ec93"},{"id":"CL-cell","color":"#ec93e1"}]}]}}