| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-64 |
Sentence |
denotes |
Complete biosynthetic pathway to the antidiabetic drug acarbose. |
| T2 |
65-176 |
Sentence |
denotes |
Acarbose is a bacterial-derived α-glucosidase inhibitor clinically used to treat patients with type 2 diabetes. |
| T3 |
177-272 |
Sentence |
denotes |
As type 2 diabetes is on the rise worldwide, the market demand for acarbose has also increased. |
| T4 |
273-375 |
Sentence |
denotes |
Despite its significant therapeutic importance, how it is made in nature is not completely understood. |
| T5 |
376-578 |
Sentence |
denotes |
Here, we report the complete biosynthetic pathway to acarbose and its structural components, GDP-valienol and O-4-amino-(4,6-dideoxy-α-D-glucopyranosyl)-(1→4)-O-α-D-glucopyranosyl-(1→4)-D-glucopyranose. |
| T6 |
579-873 |
Sentence |
denotes |
GDP-valienol is derived from valienol 7-phosphate, catalyzed by three cyclitol modifying enzymes, whereas O-4-amino-(4,6-dideoxy-α-D-glucopyranosyl)-(1→4)-O-α-D-glucopyranosyl-(1→4)-D-glucopyranose is produced from dTDP-4-amino-4,6-dideoxy-D-glucose and maltose by the glycosyltransferase AcbI. |
| T7 |
874-1045 |
Sentence |
denotes |
The final assembly process is catalyzed by a pseudoglycosyltransferase enzyme, AcbS, which is a homologue of AcbI but catalyzes the formation of a non-glycosidic C-N bond. |
| T8 |
1046-1190 |
Sentence |
denotes |
This study clarifies all previously unknown steps in acarbose biosynthesis and establishes a complete pathway to this high value pharmaceutical. |
