PubMed:3567987 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/3567987","sourcedb":"PubMed","sourceid":"3567987","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/3567987","text":"Structural characterisation of an anti-complementary arabinogalactan from the roots of Angelica acutiloba Kitagawa.\nAn anti-complementary arabinogalactan (AGIIb-1), isolated from the roots of Angelica acutiloba Kitagawa, has been subjected to methylation analysis, digestion with alpha-L-arabinofuranosidase, controlled Smith-degradation, and partial acid hydrolysis. AGIIb-1 consisted of arabinose, galactose, rhamnose, galacturonic acid, and glucuronic acid in the molar ratios 1.8-2.2:1.0:0.2-0.3:0.2-0.4:0.1. AGIIb-1 contained mainly an arabino-3,6-galactan moiety, and most of the Ara was present as alpha-L-arabinofuranosyl residues in the non-reducing terminals and the highly polymerised and branched side-chains which were attached mainly to positions 3 and 6 of (1----6)- and (1----3)-linked Gal, respectively. Some Ara-containing chains were also attached to (1----4)-linked Gal residues. The 13C-n.m.r. data for AGIIb-1 showed that the Galp was beta. Mild acid hydrolysis of AGIIb-1 yielded several linear and highly branched arabino-oligosaccharides, a neutral arabinogalactan, and two acidic arabinogalactans. Some arabino-oligosaccharides contained a (1----4)-linked Arap at the reducing terminal. The neutral arabinogalactan contained (1----3)-, (1----4)-, and (1----6)-linked and 3,6-di-O-substituted Gal, whereas the acidic arabinogalactans contained, in addition, non-reducing terminal GlcA, (1----4)-linked GalA, and 2,4-di-O-substituted Rha. The anti-complementary activity was decreased when AGIIb-1 was partially hydrolysed with mild acid (10mM HCl, 100 degrees, 10 min), but treatment with exo-alpha-L-arabinofuranosidase markedly enhanced the activity.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":115},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":116,"end":367},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":368,"end":512},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":513,"end":820},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":821,"end":899},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":900,"end":962},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":963,"end":1123},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1124,"end":1212},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1213,"end":1462},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1463,"end":1677},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":115},"obj":"Sentence"},{"id":"T2","span":{"begin":116,"end":367},"obj":"Sentence"},{"id":"T3","span":{"begin":368,"end":512},"obj":"Sentence"},{"id":"T4","span":{"begin":513,"end":820},"obj":"Sentence"},{"id":"T5","span":{"begin":821,"end":899},"obj":"Sentence"},{"id":"T6","span":{"begin":900,"end":962},"obj":"Sentence"},{"id":"T7","span":{"begin":963,"end":1123},"obj":"Sentence"},{"id":"T8","span":{"begin":1124,"end":1212},"obj":"Sentence"},{"id":"T9","span":{"begin":1213,"end":1462},"obj":"Sentence"},{"id":"T10","span":{"begin":1463,"end":1677},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":87,"end":105},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":192,"end":210},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":586,"end":589},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":826,"end":829},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"55605"},{"id":"A2","pred":"db_id","subj":"T2","obj":"55605"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9225"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9225"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T4","pred":"source","obj":"NCBITAXON"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":400,"end":409},"obj":"https://glytoucan.org/Structures/Glycans/G65889KE"},{"id":"T2","span":{"begin":400,"end":409},"obj":"https://glytoucan.org/Structures/Glycans/G68158BT"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":400,"end":409},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":467,"end":472},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0003655"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#93ecc5","default":true},{"id":"NCBITAXON","color":"#ecab93"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#9395ec"},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#afec93"},{"id":"Anatomy-UBERON","color":"#ec93c9"}]}]}}