| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-115 |
Sentence |
denotes |
Interaction between L-threonine dehydrogenase and aminoacetone synthetase and mechanism of aminoacetone production. |
| T2 |
116-236 |
Sentence |
denotes |
A mixture of threonine dehydrogenase and aminoacetone synthetase will catalyze the conversion of L-threonine to glycine. |
| T3 |
237-352 |
Sentence |
denotes |
The overall reaction likely involves the conversion of L-threonine, NAD+, and CoA to glycine, NADH, and acetyl-CoA. |
| T4 |
353-502 |
Sentence |
denotes |
Physical separation of L-threonine dehydrogenase from aminoacetone synthetase results in the formation of aminoacetone and CO2 from their substrates. |
| T5 |
503-671 |
Sentence |
denotes |
A physical interaction between threonine dehydrogenase and aminoacetone synthetase has been demonstrated by gel permeation chromatography and fluorescence polarization. |
| T6 |
672-1018 |
Sentence |
denotes |
Polarization of fluorescence measurements of threonine dehydrogenase and aminoacetone synthetase labeled with fluorescein isothiocyanate indicated the formation of a soluble active complex, with an apparent dissociation constant (Kd) of 5-10 nM and an apparent stoichiometry of 2 aminoacetone synthetase dimers/1 threonine dehydrogenase tetramer. |
| T7 |
1019-1145 |
Sentence |
denotes |
Chemical experiments have identified aminoacetone as the enzymatic product of L-threonine dehydrogenase acting on L-threonine. |
| T8 |
1146-1263 |
Sentence |
denotes |
These experiments involved trapping pyrrole derivatives, [3H]NaBH4 reduction, and coupling with plasma amine oxidase. |
| T9 |
1264-1423 |
Sentence |
denotes |
Kinetic experiments also showed NADH, CO2, and aminoacetone to inhibit threonine dehydrogenase in a manner consistent with an ordered Bi-Ter kinetic mechanism. |
| T10 |
1424-1546 |
Sentence |
denotes |
NAD+ is the lead substrate followed by threonine, and the products are released in the order: CO2, aminoacetone, and NADH. |
