PubMed:3511969 JSONTXT

{"project":"GlyCosmos15-Species-PubTator","denotations":[{"id":"17","span":{"begin":408,"end":442},"obj":"Species"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":634,"end":637},"obj":"Glycan"},{"id":"T2","span":{"begin":693,"end":696},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G90562PB"},{"id":"A3","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G90562PB"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G90562PB"},{"id":"A4","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G90562PB"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":798,"end":814},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0041092"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":642,"end":645},"obj":"Disease"},{"id":"T2","span":{"begin":706,"end":709},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0009281"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0009281"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":634,"end":637},"obj":"Glycan"},{"id":"T2","span":{"begin":693,"end":696},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G90562PB"},{"id":"A3","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G90562PB"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G90562PB"},{"id":"A4","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G90562PB"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"GlyCosmos15-HP","denotations":[{"id":"T1","span":{"begin":798,"end":814},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0041092"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":103,"end":108},"obj":"Body_part"},{"id":"T3","span":{"begin":109,"end":114},"obj":"Body_part"},{"id":"T4","span":{"begin":150,"end":169},"obj":"Body_part"},{"id":"T5","span":{"begin":299,"end":304},"obj":"Body_part"},{"id":"T7","span":{"begin":385,"end":390},"obj":"Body_part"},{"id":"T8","span":{"begin":883,"end":902},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000119"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0022303"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_6004475"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0001359"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0000119"},{"id":"A6","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0022303"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_6004475"},{"id":"A8","pred":"uberon_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/UBERON_0001359"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":170},"obj":"Sentence"},{"id":"T2","span":{"begin":171,"end":342},"obj":"Sentence"},{"id":"T3","span":{"begin":343,"end":503},"obj":"Sentence"},{"id":"T4","span":{"begin":504,"end":599},"obj":"Sentence"},{"id":"T5","span":{"begin":600,"end":791},"obj":"Sentence"},{"id":"T6","span":{"begin":792,"end":949},"obj":"Sentence"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"GlyCosmos15-NCBITAXON","denotations":[{"id":"T1","span":{"begin":408,"end":432},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":741,"end":752},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"37931"},{"id":"A2","pred":"db_id","subj":"T2","obj":"3704"}],"namespaces":[{"prefix":"_base","uri":"https://glycosmos.org/organisms/"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"GlyCosmos15-FMA","denotations":[{"id":"T1","span":{"begin":150,"end":169},"obj":"Body_part"},{"id":"T2","span":{"begin":883,"end":902},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"FMA:20935"},{"id":"A2","pred":"db_id","subj":"T2","obj":"FMA:20935"}],"namespaces":[{"prefix":"FMA","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":150,"end":169},"obj":"Body_part"},{"id":"T2","span":{"begin":883,"end":902},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000499"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000499"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":408,"end":432},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":741,"end":752},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"37931"},{"id":"A2","pred":"db_id","subj":"T2","obj":"3704"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":150,"end":169},"obj":"Body_part"},{"id":"T2","span":{"begin":883,"end":902},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000499"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000499"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":103,"end":108},"obj":"Body_part"},{"id":"T3","span":{"begin":109,"end":114},"obj":"Body_part"},{"id":"T4","span":{"begin":150,"end":169},"obj":"Body_part"},{"id":"T5","span":{"begin":299,"end":304},"obj":"Body_part"},{"id":"T7","span":{"begin":385,"end":390},"obj":"Body_part"},{"id":"T8","span":{"begin":883,"end":902},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000119"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0022303"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_6004475"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0001359"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0000119"},{"id":"A6","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0022303"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_6004475"},{"id":"A8","pred":"uberon_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/UBERON_0001359"}],"text":"Sensitive enzyme-immunostaining and densitometric determination of ganglio-series gangliosides on thin-layer plate: pmol detection of gangliosides in cerebrospinal fluid.\nAn immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods."}