PubMed:3498535 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/3498535","sourcedb":"PubMed","sourceid":"3498535","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/3498535","text":"New n.m.r.-spectroscopic approaches for structural studies of polysaccharides: application to the Haemophilus influenzae type a capsular polysaccharide.\nThe extension of several modern nuclear magnetic resonance (n.m.r.) spectroscopic techniques to polysaccharides is discussed and illustrated, using the native Haemophilus influenzae type a capsular polysaccharide. These techniques provide for the unambiguous assignment of all n.m.r. resonances (1H, 13C, and 31P) via high-sensitivity homonuclear and 1H-detected heteronuclear correlations, and they are capable of locating the intersaccharide linkages (both O-linked and phosphoric diester-linked) and appended groups (e.g. O-acetyl groups). To illustrate the power and sensitivity of these methods, a 10-mg sample of the H. Influenzae type a polysaccharide (repeat unit mol. wt. = 376) was studied. The combined acquisition time for the two-dimensional 1H-13C correlation data (one-bond and multiple-bond), the 1H-31P correlation data, and the 1H-1H (homonuclear Hartmann-Hahn) data was approximately 18 h.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":152},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":153,"end":366},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":367,"end":695},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":696,"end":778},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":779,"end":853},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":854,"end":1061},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":152},"obj":"Sentence"},{"id":"T2","span":{"begin":153,"end":366},"obj":"Sentence"},{"id":"T3","span":{"begin":367,"end":695},"obj":"Sentence"},{"id":"T4","span":{"begin":696,"end":778},"obj":"Sentence"},{"id":"T5","span":{"begin":779,"end":853},"obj":"Sentence"},{"id":"T6","span":{"begin":854,"end":1061},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":98,"end":120},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":312,"end":334},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"727"},{"id":"A2","pred":"db_id","subj":"T2","obj":"727"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":110,"end":120},"obj":"Disease"},{"id":"T2","span":{"begin":324,"end":334},"obj":"Disease"},{"id":"T3","span":{"begin":779,"end":789},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005812"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005812"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0005812"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"},{"subj":"T3","pred":"source","obj":"mondo_disease"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":157,"end":166},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_2000106"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#93ecc3","default":true},{"id":"NCBITAXON","color":"#eca993"},{"id":"mondo_disease","color":"#9396ec"},{"id":"Anatomy-UBERON","color":"#b0ec93"}]}]}}