PubMed:3485199 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/3485199","sourcedb":"PubMed","sourceid":"3485199","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/3485199","text":"Recognition of cloned influenza virus hemagglutinin gene products by cytotoxic T lymphocytes.\nThe influenza A virus hemagglutinin (HA) is an integral membrane glycoprotein expressed in large quantities on infected cell surfaces and is known to serve as a target antigen for influenza virus-specific cytotoxic T lymphocytes (CTL). Despite the fact that HAs derived from different influenza A virus subtypes are serologically non-cross-reactive, the HA has been implicated by previous experiments to be a target antigen for the subset of T cells capable of lysing cells infected with any human influenza A subtype (cross-reactive CTL). To directly determine whether the HA is recognized by cross-reactive CTL, we used vaccinia virus recombinants containing DNA copies of the PR8 (A/Puerto Rico/8/34) (H1N1) or JAP (A/JAP/305) (H2N2) HA genes. When these viruses were used to stimulate HA-specific CTL and to sensitize target cells for lysis by HA-specific CTL, we found no evidence for HA recognition by cross-reactive CTL aside from a relatively small degree of cross-reactivity between H1 and H2 HAs. Results of unlabeled target inhibition studies were consistent with the conclusion that the HA is, at most, only a minor target antigen for cross-reactive CTL.","tracks":[{"project":"IAV-Glycan_IAV_human_2","denotations":[{"id":"T1","span":{"begin":131,"end":133},"obj":"HA"},{"id":"T2","span":{"begin":397,"end":405},"obj":"subtype"},{"id":"T3","span":{"begin":448,"end":450},"obj":"HA"},{"id":"T4","span":{"begin":604,"end":611},"obj":"subtype"},{"id":"T5","span":{"begin":668,"end":670},"obj":"HA"},{"id":"T6","span":{"begin":799,"end":803},"obj":"H1N1"},{"id":"T7","span":{"begin":831,"end":833},"obj":"HA"},{"id":"T8","span":{"begin":883,"end":885},"obj":"HA"},{"id":"T9","span":{"begin":942,"end":944},"obj":"HA"},{"id":"T10","span":{"begin":984,"end":986},"obj":"HA"},{"id":"T11","span":{"begin":1086,"end":1088},"obj":"H1"},{"id":"T12","span":{"begin":1093,"end":1095},"obj":"H2"},{"id":"T13","span":{"begin":1193,"end":1195},"obj":"HA"}],"attributes":[{"subj":"T1","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T2","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T3","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T4","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T5","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T6","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T7","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T8","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T9","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T10","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T11","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T12","pred":"source","obj":"IAV-Glycan_IAV_human_2"},{"subj":"T13","pred":"source","obj":"IAV-Glycan_IAV_human_2"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"IAV-Glycan_IAV_human_2","color":"#c393ec","default":true}]}]}}