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PubMed:34754033 JSONTXT

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GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 712-718 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T2 749-755 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T3 1044-1050 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T4 1115-1121 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T5 1571-1577 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T6 1583-1589 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 712-718 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T2 749-755 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T3 1044-1050 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T4 1115-1121 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T5 1571-1577 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL
T6 1583-1589 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 42-63 Organism denotes Clostridium botulinum 1491
T2 384-405 Organism denotes Clostridium botulinum 1491
T3 988-993 Organism denotes Alexa 149625
T4 1067-1072 Organism denotes Alexa 149625

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-92 Sentence denotes Glycan detecting tools developed from the Clostridium botulinum whole hemagglutinin complex.
T2 93-183 Sentence denotes Lectins are proteins with the ability to recognize and bind to specific glycan structures.
T3 184-350 Sentence denotes These molecules play important roles in many biological systems and are actively being studied because of their ability to detect glycan biomarkers for many diseases.
T4 351-564 Sentence denotes Hemagglutinin (HA) proteins from Clostridium botulinum type C neurotoxin complex; HA1, HA2, and HA3 are lectins that aid in the internalization of the toxin complex by binding to glycoproteins on the cell surface.
T5 565-778 Sentence denotes HA1 mutants have been previously reported, namely HA1 W176A/D271F and HA1 N278A/Q279A which are specific to galactose (Gal)/N-acetylgalactosamine (GalNAc) and N-acetylneuraminic acid (Neu5Ac) sugars, respectively.
T6 779-932 Sentence denotes In this study, we utilized HA1 mutants and expressed them in complex with HA2 WT and HA3 WT to produce glycan detecting tools with high binding affinity.
T7 933-967 Sentence denotes Particularly, two types were made:
T8 968-1122 Sentence denotes Gg and Rn. Gg is an Alexa 488 conjugated lectin complex specific to Gal and GalNAc, while Rn is an Alexa 594 conjugated lectin complex specific to Neu5Ac.
T9 1123-1262 Sentence denotes The specificities of these lectins were identified using a glycan microarray followed by competitive sugar inhibition experiments on cells.
T10 1263-1490 Sentence denotes In addition, we confirmed that Gg and Rn staining is clearly different depending on cell type, and the staining pattern of these lectins reflects the glycans present on the cell surface as shown in enzyme treatment experiments.
T11 1491-1711 Sentence denotes The availability of Gg and Rn provide us with new promising tools to study Gal, GalNAc, and Neu5Ac terminal epitopes which can aid in understanding the functional role of glycans in physiological and pathological events.

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 551-563 Body_part denotes cell surface FMA:67653
T2 1436-1448 Body_part denotes cell surface FMA:67653

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 42-63 OrganismTaxon denotes Clostridium botulinum 1491
T2 384-405 OrganismTaxon denotes Clostridium botulinum 1491
T3 988-993 OrganismTaxon denotes Alexa 149625
T4 1067-1072 OrganismTaxon denotes Alexa 149625