PubMed:34478936 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/34478936","sourcedb":"PubMed","sourceid":"34478936","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/34478936","text":"Preparation and characterization of sulfated inulin-type fructans from Jerusalem artichoke tubers and their antitumor activity.\nThe modification of polysaccharides is important for enhancing their biological activities. In this study, a pure inulin-type fructan, denoted as Jerusalem artichoke polysaccharide (P-JAP), was purified from Jerusalem artichoke tubers and modified by sulfation via treatment with a sulfur trioxide-pyridine complex to produce its sulfated derivative (S-JAP). Fourier-transform infrared spectroscopic analysis confirmed the successful introduction of sulfate groups. The inhibitory effects of S-JAP on the proliferation of human liver hepatocellular carcinoma (HepG2) cells was evaluated via a CCK-8 assay, and the pro-apoptotic effects were assessed using annexin V-FITC/PI double staining. The inhibition rates of various concentrations of S-JAP on HepG2 cells after 24, 48, and 72 h were significantly higher than those of P-JAP; moreover, S-JAP succeeded in promoting cell apoptosis. Thus, the sulfate-modified polysaccharide extracted from Jerusalem artichoke tubers was shown to exhibit effective antitumor activity with potential for further development.","tracks":[{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":71,"end":90},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":274,"end":293},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":336,"end":355},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":650,"end":655},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":1072,"end":1091},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"4233"},{"id":"A2","pred":"db_id","subj":"T2","obj":"4233"},{"id":"A3","pred":"db_id","subj":"T3","obj":"4233"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9606"},{"id":"A5","pred":"db_id","subj":"T5","obj":"4233"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T4","pred":"source","obj":"NCBITAXON"},{"subj":"T5","pred":"source","obj":"NCBITAXON"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":662,"end":686},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0007256"},{"subj":"T1","pred":"source","obj":"mondo_disease"}]},{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":91,"end":97},"obj":"http://purl.obolibrary.org/obo/MAT_0000358"},{"id":"T2","span":{"begin":356,"end":362},"obj":"http://purl.obolibrary.org/obo/MAT_0000358"},{"id":"T3","span":{"begin":656,"end":661},"obj":"http://purl.obolibrary.org/obo/MAT_0000097"},{"id":"T4","span":{"begin":1092,"end":1098},"obj":"http://purl.obolibrary.org/obo/MAT_0000358"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T2","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T3","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T4","pred":"source","obj":"Glycosmos6-MAT"}]},{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":91,"end":97},"obj":"Body_part"},{"id":"T2","span":{"begin":356,"end":362},"obj":"Body_part"},{"id":"T3","span":{"begin":656,"end":661},"obj":"Body_part"},{"id":"T4","span":{"begin":1092,"end":1098},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000358"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000358"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000097"},{"id":"A4","pred":"mat_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MAT_0000358"},{"subj":"T1","pred":"source","obj":"Anatomy-MAT"},{"subj":"T2","pred":"source","obj":"Anatomy-MAT"},{"subj":"T3","pred":"source","obj":"Anatomy-MAT"},{"subj":"T4","pred":"source","obj":"Anatomy-MAT"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":656,"end":661},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":662,"end":686},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0001402"},{"subj":"T1","pred":"source","obj":"HP-phenotype"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"NCBITAXON","color":"#ec93a8","default":true},{"id":"mondo_disease","color":"#93c2ec"},{"id":"Glycosmos6-MAT","color":"#dcec93"},{"id":"Anatomy-MAT","color":"#e293ec"},{"id":"Anatomy-UBERON","color":"#93ecc7"},{"id":"HP-phenotype","color":"#ecad93"}]}]}}