PubMed:3427582 JSONTXT

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":107},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":108,"end":421},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":422,"end":526},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":527,"end":714},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":715,"end":994},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":995,"end":1169},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1170,"end":1304},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1305,"end":1443},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1444,"end":1687},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":107},"obj":"Sentence"},{"id":"T2","span":{"begin":108,"end":421},"obj":"Sentence"},{"id":"T3","span":{"begin":422,"end":526},"obj":"Sentence"},{"id":"T4","span":{"begin":527,"end":714},"obj":"Sentence"},{"id":"T5","span":{"begin":715,"end":994},"obj":"Sentence"},{"id":"T6","span":{"begin":995,"end":1169},"obj":"Sentence"},{"id":"T7","span":{"begin":1170,"end":1304},"obj":"Sentence"},{"id":"T8","span":{"begin":1305,"end":1443},"obj":"Sentence"},{"id":"T9","span":{"begin":1444,"end":1687},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Formation of 6-O-alpha-maltosylcyclomalto-oligosaccharides by transfer action of three debranching enzymes.\nO-Maltosylcyclomaltohexaoses (G2-cG6) were formed in yields of 24.3 and 23.2 mmol from 40 mmol of alpha-maltosyl fluoride (alpha-G2F) and 90 mmol of cyclomaltohexaose (cG6) by the transfer action of pullulanase from Aerobacter aerogenes (A-pullulanase) and isoamylase from Pseudomonas amyloderamosa, respectively. These yields were three times that given by pullulanase from Bacillus acidopullulyticus (B-pullulanase). The yields of O-maltosylcyclomalto-oligosaccharides were changed according to the origin of the enzymes and the kind of cyclomalto-oligosaccharide (cG6, cG7, or cG8) used as the acceptor. By the reaction with 40 mmol of alpha-G2F and 90 mmol of cG6, 20 mmol of alpha-G2F and 30 mmol of cG7, or 40 mmol of alpha-G2F and 90 mmol of cG8, the amounts of O-maltosylcyclomalto-oligosaccharides produced and the transfer ratios of alpha-G2F to the acceptors were as follows. By A-pullulanase, 24.3 mmol of G2-cG6 was produced in a 60.8% transfer ratio, whereas the yields of G2-cG7 and G2-cG8 were 1.7 mmol (8.5%) and 8.4 mmol (21.0%), respectively. The yields of G2-cG6, G2-cG7, and G2-cG8 by B-pullulanase were 8.8 mmol (22.0%), 1.2 mmol (6.0%), and 11.7 mmol (29.3%), respectively. In the case of isoamylase, G2-cG7 (9.2 mmol, 46.0%) and G2-cG8 (20.9 mmol, 52.3%) were produced, as much as for G2-cG6 (23.2 mmol, 58.0%). It was suggested that the difference in the amounts of G2-cG6 produced by these three debranching enzymes is based on the difference in the mode of action on the alpha-G2F used as the substrate, either a transfer action or a hydrolytic action."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":324,"end":344},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":381,"end":392},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":483,"end":509},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"548"},{"id":"A2","pred":"db_id","subj":"T2","obj":"286"},{"id":"A3","pred":"db_id","subj":"T3","obj":"28030"}],"text":"Formation of 6-O-alpha-maltosylcyclomalto-oligosaccharides by transfer action of three debranching enzymes.\nO-Maltosylcyclomaltohexaoses (G2-cG6) were formed in yields of 24.3 and 23.2 mmol from 40 mmol of alpha-maltosyl fluoride (alpha-G2F) and 90 mmol of cyclomaltohexaose (cG6) by the transfer action of pullulanase from Aerobacter aerogenes (A-pullulanase) and isoamylase from Pseudomonas amyloderamosa, respectively. These yields were three times that given by pullulanase from Bacillus acidopullulyticus (B-pullulanase). The yields of O-maltosylcyclomalto-oligosaccharides were changed according to the origin of the enzymes and the kind of cyclomalto-oligosaccharide (cG6, cG7, or cG8) used as the acceptor. By the reaction with 40 mmol of alpha-G2F and 90 mmol of cG6, 20 mmol of alpha-G2F and 30 mmol of cG7, or 40 mmol of alpha-G2F and 90 mmol of cG8, the amounts of O-maltosylcyclomalto-oligosaccharides produced and the transfer ratios of alpha-G2F to the acceptors were as follows. By A-pullulanase, 24.3 mmol of G2-cG6 was produced in a 60.8% transfer ratio, whereas the yields of G2-cG7 and G2-cG8 were 1.7 mmol (8.5%) and 8.4 mmol (21.0%), respectively. The yields of G2-cG6, G2-cG7, and G2-cG8 by B-pullulanase were 8.8 mmol (22.0%), 1.2 mmol (6.0%), and 11.7 mmol (29.3%), respectively. In the case of isoamylase, G2-cG7 (9.2 mmol, 46.0%) and G2-cG8 (20.9 mmol, 52.3%) were produced, as much as for G2-cG6 (23.2 mmol, 58.0%). It was suggested that the difference in the amounts of G2-cG6 produced by these three debranching enzymes is based on the difference in the mode of action on the alpha-G2F used as the substrate, either a transfer action or a hydrolytic action."}