PubMed:33837264
Annnotations
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 512-516 | Glycan | denotes | GT1b | https://api.glycosmos.org/wurcs2image/latest/png/binary/G40183QN |
| T2 | 1130-1134 | Glycan | denotes | GT1b | https://api.glycosmos.org/wurcs2image/latest/png/binary/G40183QN |
mondo_disease
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 466-470 | Disease | denotes | HC/A | http://purl.obolibrary.org/obo/MONDO_0018902 |
| T2 | 593-597 | Disease | denotes | HC/A | http://purl.obolibrary.org/obo/MONDO_0018902 |
| T3 | 1110-1114 | Disease | denotes | HC/A | http://purl.obolibrary.org/obo/MONDO_0018902 |
| T4 | 1271-1275 | Disease | denotes | HC/A | http://purl.obolibrary.org/obo/MONDO_0018902 |
| T5 | 1440-1444 | Disease | denotes | HC/A | http://purl.obolibrary.org/obo/MONDO_0018902 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 512-516 | Glycan | denotes | GT1b | https://api.glycosmos.org/wurcs2image/latest/png/binary/G40183QN |
| T2 | 1130-1134 | Glycan | denotes | GT1b | https://api.glycosmos.org/wurcs2image/latest/png/binary/G40183QN |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 512-516 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GT1b | http://www.glycoepitope.jp/epitopes/EP0067 |
| T2 | 1130-1134 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GT1b | http://www.glycoepitope.jp/epitopes/EP0067 |
GlyCosmos15-HP
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 353-362 | Phenotype | denotes | paralysis | HP:0003470 |
GlyCosmos15-CL
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 77-87 | Cell | denotes | fibroblast | http://purl.obolibrary.org/obo/CL:0000057 |
| T2 | 416-424 | Cell | denotes | neuronal | http://purl.obolibrary.org/obo/CL:0000540 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 77-87 | Body_part | denotes | fibroblast | http://purl.obolibrary.org/obo/CL_0000057 |
GlyCosmos15-Taxon
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 20-41 | Organism | denotes | clostridium botulinum | 1491 |
| T2 | 165-186 | Organism | denotes | Clostridium botulinum | 1491 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-164 | Sentence | denotes | Characterization of clostridium botulinum neurotoxin serotype A (BoNT/A) and fibroblast growth factor receptor interactions using novel receptor dimerization assay. |
| T2 | 165-363 | Sentence | denotes | Clostridium botulinum neurotoxin serotype A (BoNT/A) is a potent neurotoxin that serves as an effective therapeutic for several neuromuscular disorders via induction of temporary muscular paralysis. |
| T3 | 364-568 | Sentence | denotes | Specific binding and internalization of BoNT/A into neuronal cells is mediated by its binding domain (HC/A), which binds to gangliosides, including GT1b, and protein cell surface receptors, including SV2. |
| T4 | 569-630 | Sentence | denotes | Previously, recombinant HC/A was also shown to bind to FGFR3. |
| T5 | 631-1068 | Sentence | denotes | As FGFR dimerization is an indirect measure of ligand-receptor binding, an FCS & TIRF receptor dimerization assay was developed to measure rHC/A-induced dimerization of fluorescently tagged FGFR subtypes (FGFR1-3) in cells. rHC/A dimerized FGFR subtypes in the rank order FGFR3c (EC50 ≈ 27 nM) > FGFR2b (EC50 ≈ 70 nM) > FGFR1c (EC50 ≈ 163 nM); rHC/A dimerized FGFR3c with similar potency as the native FGFR3c ligand, FGF9 (EC50 ≈ 18 nM). |
| T6 | 1069-1186 | Sentence | denotes | Mutating the ganglioside binding site in HC/A, or removal of GT1b from the media, resulted in decreased dimerization. |
| T7 | 1187-1532 | Sentence | denotes | Interestingly, reduced dimerization was also observed with an SV2 mutant variant of HC/A. Overall, the results suggest that the FCS & TIRF receptor dimerization assay can assess FGFR dimerization with known and novel ligands and support a model wherein HC/A, either directly or indirectly, interacts with FGFRs and induces receptor dimerization. |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 512-516 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GT1b | http://www.glycoepitope.jp/epitopes/EP0067 |
| T2 | 1130-1134 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GT1b | http://www.glycoepitope.jp/epitopes/EP0067 |
GlyCosmos15-FMA
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 77-87 | Body_part | denotes | fibroblast | FMA:63877 |
| T2 | 530-542 | Body_part | denotes | cell surface | FMA:67653 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 20-41 | OrganismTaxon | denotes | clostridium botulinum | 1491 |
| T2 | 165-186 | OrganismTaxon | denotes | Clostridium botulinum | 1491 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 77-87 | Body_part | denotes | fibroblast | http://purl.obolibrary.org/obo/CL_0000057 |
HP-phenotype
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 353-362 | Phenotype | denotes | paralysis | HP:0003470 |
CL-cell
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 77-87 | Cell | denotes | fibroblast | http://purl.obolibrary.org/obo/CL:0000057 |
| T2 | 416-424 | Cell | denotes | neuronal | http://purl.obolibrary.org/obo/CL:0000540 |