| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-106 |
Sentence |
denotes |
Reproductive phasiRNA loci and DICER-LIKE5, but not microRNA loci, diversified in monocotyledonous plants. |
| T2 |
107-325 |
Sentence |
denotes |
In monocots other than maize (Zea mays) and rice (Oryza sativa), the repertoire and diversity of microRNAs (miRNAs) and the populations of phased, secondary, small interfering RNAs (phasiRNAs) are poorly characterized. |
| T3 |
326-588 |
Sentence |
denotes |
To remedy this, we sequenced small RNAs (sRNA) from vegetative and dissected inflorescence tissue in 28 phylogenetically diverse monocots and from several early-diverging angiosperm lineages, as well as publicly available data from 10 additional monocot species. |
| T4 |
589-851 |
Sentence |
denotes |
We annotated miRNAs, small interfering RNAs (siRNAs) and phasiRNAs across the monocot phylogeny, identifying miRNAs apparently lost or gained in the grasses relative to other monocot families, as well as a number of transfer RNA fragments misannotated as miRNAs. |
| T5 |
852-1077 |
Sentence |
denotes |
Using our miRNA database cleaned of these misannotations, we identified conservation at the 8th, 9th, 19th, and 3'-end positions that we hypothesize are signatures of selection for processing, targeting, or Argonaute sorting. |
| T6 |
1078-1192 |
Sentence |
denotes |
We show that 21-nucleotide (nt) reproductive phasiRNAs are far more numerous in grass genomes than other monocots. |
| T7 |
1193-1382 |
Sentence |
denotes |
Based on sequenced monocot genomes and transcriptomes, DICER-LIKE5, important to 24-nt phasiRNA biogenesis, likely originated via gene duplication before the diversification of the grasses. |
| T8 |
1383-1598 |
Sentence |
denotes |
This curated database of phylogenetically diverse monocot miRNAs, siRNAs, and phasiRNAs represents a large collection of data that should facilitate continued exploration of sRNA diversification in flowering plants. |
