PubMed:3370650 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/3370650","sourcedb":"PubMed","sourceid":"3370650","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/3370650","text":"Order of enzymic incorporation of O-methyl groups into the O-methyl-D-glucose-containing polysaccharide of Mycobacterium smegmatis. A tritium-labelling study.\nThe order of enzymic incorporation of O-methyl groups into the O-methyl-D-glucose-containing polysaccharide (MGP) of Mycobacterium smegmatis, 3MG(J)----G(I)----G(H) ----G(G)----6MG(F)----(GMG)9(E)----[G(L)----G(D)]----G(C) ----[G(K)----G(B)]----G(A)----Ga, where G is D-glucose, 3MG is 3-O-methyl-D-glucose, 6MG is 6-O-methyl-D-glucose, and Ga is D-glyceric acid, was studied by incubating cultures of M. smegmatis with L-[3H-Me]methionine for various times. MGP was then extracted from the cells, and relative radioactivities of residues D, (E + F)average and J, or of D, E average, F, and J, were determined. Tritium-labelling of these residues increased in the reducing-to-nonreducing residue direction, the steepness of the gradient becoming more shallow with increasing incubation time. The results are consistent with a biosynthetic mechanism that involves sequential addition of O-methyl groups to residues of the pre-formed D-glucan, in the reducing-to-nonreducing residue direction.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":131},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":132,"end":158},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":159,"end":617},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":618,"end":769},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":770,"end":950},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":951,"end":1150},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":131},"obj":"Sentence"},{"id":"T2","span":{"begin":132,"end":158},"obj":"Sentence"},{"id":"T3","span":{"begin":159,"end":617},"obj":"Sentence"},{"id":"T4","span":{"begin":618,"end":769},"obj":"Sentence"},{"id":"T5","span":{"begin":770,"end":950},"obj":"Sentence"},{"id":"T6","span":{"begin":951,"end":1150},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":107,"end":130},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":276,"end":299},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"1772"},{"id":"A2","pred":"db_id","subj":"T2","obj":"1772"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":70,"end":77},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"},{"id":"T2","span":{"begin":233,"end":240},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"},{"id":"T3","span":{"begin":429,"end":436},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"},{"id":"T4","span":{"begin":458,"end":465},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"},{"id":"T5","span":{"begin":487,"end":494},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":70,"end":77},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":233,"end":240},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":429,"end":436},"obj":"Glycan_Motif"},{"id":"T4","span":{"begin":458,"end":465},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":487,"end":494},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"id":"A4","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#c993ec","default":true},{"id":"NCBITAXON","color":"#93ecaf"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#ec9493"},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#93abec"}]}]}}