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CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 746-750 Cell denotes band http://purl.obolibrary.org/obo/CL:0000560
T2 1654-1658 Cell denotes band http://purl.obolibrary.org/obo/CL:0000560

sentences

Id Subject Object Predicate Lexical cue
T1 0-50 Sentence denotes Characterization of ovarian gonadotropin receptor.
T2 51-95 Sentence denotes Monomer and associated form of the receptor.
T3 96-282 Sentence denotes We have purified luteinizing hormone/human choriogonadotropin (hCG) receptor from rat ovary by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose chromatography.
T4 283-458 Sentence denotes The purified receptor, previously identified as a single protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Kusuda, S., and Dufau, M.L. (1986) J.
T5 459-464 Sentence denotes Biol.
T6 465-470 Sentence denotes Chem.
T7 471-653 Sentence denotes 261, 16161-16168), was further characterized by radioiodination with 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycouril, and column chromatography on wheat germ lectin-Sepharose.
T8 654-766 Sentence denotes Autoradiography of SDS-PAGE analysis under reducing conditions showed a single radiolabeled band of Mr = 80,000.
T9 767-857 Sentence denotes The radioiodinated receptors treated with peptide:N-glycosidase F migrated at Mr = 54,000.
T10 858-1056 Sentence denotes Treatment with neuraminidase alone caused only a minor reduction in molecular weight, and subsequent treatment with endo-alpha-N-acetyl-D-galactosaminidase had little further effect on the receptor.
T11 1057-1206 Sentence denotes When the radioiodinated receptor was analyzed by fast protein liquid chromatography, a single broad peak was eluted with Mr of approximately 350,000.
T12 1207-1360 Sentence denotes The higher Mr of radioiodinated receptors than that of native receptors (Mr = 190,000 dimeric form) could be due to the aggregation of labeled molecules.
T13 1361-1436 Sentence denotes These complexes dissociated into the monomeric form in the presence of SDS.
T14 1437-1616 Sentence denotes To determine whether the monomers can bind hormone, the purified unlabeled receptors resolved with SDS were electroblotted to nitrocellulose membranes and incubated with 125I-hCG.
T15 1617-1735 Sentence denotes Autoradiograms of the blots showed a band of monomer (Mr = 78,000) as well as one of dimer (Mr approximately 150,000).
T16 1736-1976 Sentence denotes These studies have demonstrated that the luteinizing hormone/hCG receptors are predominantly N-linked glycosylated and suggest that the native receptor is a dimer of identical hormone binding subunits associated by noncovalent interactions.
T17 1977-2105 Sentence denotes Although the individual subunits can bind hormone, it is conceivable that the dimeric form is necessary for signal transduction.
T1 0-50 Sentence denotes Characterization of ovarian gonadotropin receptor.
T2 51-95 Sentence denotes Monomer and associated form of the receptor.
T3 96-282 Sentence denotes We have purified luteinizing hormone/human choriogonadotropin (hCG) receptor from rat ovary by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose chromatography.
T4 283-458 Sentence denotes The purified receptor, previously identified as a single protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Kusuda, S., and Dufau, M.L. (1986) J.
T5 459-464 Sentence denotes Biol.
T6 465-470 Sentence denotes Chem.
T7 471-653 Sentence denotes 261, 16161-16168), was further characterized by radioiodination with 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycouril, and column chromatography on wheat germ lectin-Sepharose.
T8 654-766 Sentence denotes Autoradiography of SDS-PAGE analysis under reducing conditions showed a single radiolabeled band of Mr = 80,000.
T9 767-857 Sentence denotes The radioiodinated receptors treated with peptide:N-glycosidase F migrated at Mr = 54,000.
T10 858-1056 Sentence denotes Treatment with neuraminidase alone caused only a minor reduction in molecular weight, and subsequent treatment with endo-alpha-N-acetyl-D-galactosaminidase had little further effect on the receptor.
T11 1057-1206 Sentence denotes When the radioiodinated receptor was analyzed by fast protein liquid chromatography, a single broad peak was eluted with Mr of approximately 350,000.
T12 1207-1360 Sentence denotes The higher Mr of radioiodinated receptors than that of native receptors (Mr = 190,000 dimeric form) could be due to the aggregation of labeled molecules.
T13 1361-1436 Sentence denotes These complexes dissociated into the monomeric form in the presence of SDS.
T14 1437-1616 Sentence denotes To determine whether the monomers can bind hormone, the purified unlabeled receptors resolved with SDS were electroblotted to nitrocellulose membranes and incubated with 125I-hCG.
T15 1617-1735 Sentence denotes Autoradiograms of the blots showed a band of monomer (Mr = 78,000) as well as one of dimer (Mr approximately 150,000).
T16 1736-1976 Sentence denotes These studies have demonstrated that the luteinizing hormone/hCG receptors are predominantly N-linked glycosylated and suggest that the native receptor is a dimer of identical hormone binding subunits associated by noncovalent interactions.
T17 1977-2105 Sentence denotes Although the individual subunits can bind hormone, it is conceivable that the dimeric form is necessary for signal transduction.

Lectin-Jamboree-Sentence

Id Subject Object Predicate Lexical cue
T1 0-50 Sentence denotes Characterization of ovarian gonadotropin receptor.
T2 51-95 Sentence denotes Monomer and associated form of the receptor.
T3 96-282 Sentence denotes We have purified luteinizing hormone/human choriogonadotropin (hCG) receptor from rat ovary by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose chromatography.
T4 283-766 Sentence denotes The purified receptor, previously identified as a single protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Kusuda, S., and Dufau, M.L. (1986) J. Biol. Chem. 261, 16161-16168), was further characterized by radioiodination with 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycouril, and column chromatography on wheat germ lectin-Sepharose. Autoradiography of SDS-PAGE analysis under reducing conditions showed a single radiolabeled band of Mr = 80,000.
T5 767-857 Sentence denotes The radioiodinated receptors treated with peptide:N-glycosidase F migrated at Mr = 54,000.
T6 858-1056 Sentence denotes Treatment with neuraminidase alone caused only a minor reduction in molecular weight, and subsequent treatment with endo-alpha-N-acetyl-D-galactosaminidase had little further effect on the receptor.
T7 1057-1206 Sentence denotes When the radioiodinated receptor was analyzed by fast protein liquid chromatography, a single broad peak was eluted with Mr of approximately 350,000.
T8 1207-1360 Sentence denotes The higher Mr of radioiodinated receptors than that of native receptors (Mr = 190,000 dimeric form) could be due to the aggregation of labeled molecules.
T9 1361-1436 Sentence denotes These complexes dissociated into the monomeric form in the presence of SDS.
T10 1437-1616 Sentence denotes To determine whether the monomers can bind hormone, the purified unlabeled receptors resolved with SDS were electroblotted to nitrocellulose membranes and incubated with 125I-hCG.
T11 1617-1735 Sentence denotes Autoradiograms of the blots showed a band of monomer (Mr = 78,000) as well as one of dimer (Mr approximately 150,000).
T12 1736-1976 Sentence denotes These studies have demonstrated that the luteinizing hormone/hCG receptors are predominantly N-linked glycosylated and suggest that the native receptor is a dimer of identical hormone binding subunits associated by noncovalent interactions.
T13 1977-2105 Sentence denotes Although the individual subunits can bind hormone, it is conceivable that the dimeric form is necessary for signal transduction.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 133-138 OrganismTaxon denotes human 9606
T2 178-181 OrganismTaxon denotes rat 10114|10116
T4 221-226 OrganismTaxon denotes wheat 4565
T5 625-630 OrganismTaxon denotes wheat 4565

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 182-187 Body_part denotes ovary http://purl.obolibrary.org/obo/UBERON_0000992
T2 1578-1587 Body_part denotes membranes http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158