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PubMed:3335514 JSONTXT

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GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 599-620 Glycan_Motif denotes chondroitin 6-sulfate https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G34992DF
T2 599-610 Glycan_Motif denotes chondroitin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT
T3 1518-1529 Glycan_Motif denotes chondroitin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 599-620 http://www.glycoepitope.jp/epitopes/EP0083 denotes chondroitin 6-sulfate
T2 599-610 http://www.glycoepitope.jp/epitopes/EP0081 denotes chondroitin
T3 1518-1529 http://www.glycoepitope.jp/epitopes/EP0081 denotes chondroitin

sentences

Id Subject Object Predicate Lexical cue
T1 0-96 Sentence denotes Sulfated proteoglycans and sulfated proteins in guinea pig megakaryocytes and platelets in vivo.
T2 97-159 Sentence denotes Relevance to megakaryocyte maturation and platelet activation.
T3 160-258 Sentence denotes This study has examined changes in proteoglycan synthesis during megakaryocyte maturation in vivo.
T4 259-372 Sentence denotes Guinea pigs were injected with Na235SO4, and megakaryocytes and platelets were isolated from 3 h to 5 days later.
T5 373-579 Sentence denotes The proteoglycans and other sulfated molecules in both cells were characterized at each time point by gel filtration, ion-exchange chromatography, gel electrophoresis, and chemical and enzymatic digestions.
T6 580-678 Sentence denotes Two populations of chondroitin 6-sulfate proteoglycans were found by DEAE-Sephacel chromatography.
T7 679-805 Sentence denotes The major fraction was eluted with 4 M guanidine hydrochloride and the minor fraction with 4 M guanidine HCl, 2% Triton X-100.
T8 806-1016 Sentence denotes The Kav of the major proteoglycan peak in the platelets at 1 day after injection was 0.18-0.20 on Sepharose CL-6B and decreased gradually to 0.12 by 3 days, when proteoglycan radioactivity per cell was maximal.
T9 1017-1095 Sentence denotes The peak for megakaryocyte proteoglycans at 3 h was broad, with Kav = 0.1-0.2.
T10 1096-1226 Sentence denotes The appearance of different portions of the proteoglycan peak in platelets coincided with their disappearance from megakaryocytes.
T11 1227-1294 Sentence denotes Proteoglycan size was a function of glycosaminoglycan chain length.
T12 1295-1453 Sentence denotes The proteoglycans eluted with Triton X-100 from DEAE-Sephacel (Kav = 0.04-0.07 on Sepharose CL-6B) were not labeled in platelets until 2 days after injection.
T13 1454-1587 Sentence denotes Our data suggest that megakaryocytes synthesize different-sized chondroitin sulfate proteoglycans at different stages of development.
T14 1588-1721 Sentence denotes The proteoglycans of the major fraction were released from platelets in response to thrombin, and a small amount was released by ADP.
T15 1722-1804 Sentence denotes The proteoglycans of the Triton X-100 eluate were not released by thrombin or ADP.
T16 1805-1953 Sentence denotes About 20% of the sulfate radioactivity was incorporated into molecules that appear to be sulfated proteins and were not released by thrombin or ADP.
T1 0-96 Sentence denotes Sulfated proteoglycans and sulfated proteins in guinea pig megakaryocytes and platelets in vivo.
T2 97-159 Sentence denotes Relevance to megakaryocyte maturation and platelet activation.
T3 160-258 Sentence denotes This study has examined changes in proteoglycan synthesis during megakaryocyte maturation in vivo.
T4 259-372 Sentence denotes Guinea pigs were injected with Na235SO4, and megakaryocytes and platelets were isolated from 3 h to 5 days later.
T5 373-579 Sentence denotes The proteoglycans and other sulfated molecules in both cells were characterized at each time point by gel filtration, ion-exchange chromatography, gel electrophoresis, and chemical and enzymatic digestions.
T6 580-678 Sentence denotes Two populations of chondroitin 6-sulfate proteoglycans were found by DEAE-Sephacel chromatography.
T7 679-805 Sentence denotes The major fraction was eluted with 4 M guanidine hydrochloride and the minor fraction with 4 M guanidine HCl, 2% Triton X-100.
T8 806-1016 Sentence denotes The Kav of the major proteoglycan peak in the platelets at 1 day after injection was 0.18-0.20 on Sepharose CL-6B and decreased gradually to 0.12 by 3 days, when proteoglycan radioactivity per cell was maximal.
T9 1017-1095 Sentence denotes The peak for megakaryocyte proteoglycans at 3 h was broad, with Kav = 0.1-0.2.
T10 1096-1226 Sentence denotes The appearance of different portions of the proteoglycan peak in platelets coincided with their disappearance from megakaryocytes.
T11 1227-1294 Sentence denotes Proteoglycan size was a function of glycosaminoglycan chain length.
T12 1295-1453 Sentence denotes The proteoglycans eluted with Triton X-100 from DEAE-Sephacel (Kav = 0.04-0.07 on Sepharose CL-6B) were not labeled in platelets until 2 days after injection.
T13 1454-1587 Sentence denotes Our data suggest that megakaryocytes synthesize different-sized chondroitin sulfate proteoglycans at different stages of development.
T14 1588-1721 Sentence denotes The proteoglycans of the major fraction were released from platelets in response to thrombin, and a small amount was released by ADP.
T15 1722-1804 Sentence denotes The proteoglycans of the Triton X-100 eluate were not released by thrombin or ADP.
T16 1805-1953 Sentence denotes About 20% of the sulfate radioactivity was incorporated into molecules that appear to be sulfated proteins and were not released by thrombin or ADP.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 599-620 https://glytoucan.org/Structures/Glycans/G34992DF denotes chondroitin 6-sulfate
T2 599-610 https://glytoucan.org/Structures/Glycans/G43702JT denotes chondroitin
T3 1518-1529 https://glytoucan.org/Structures/Glycans/G43702JT denotes chondroitin

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 366-371 http://purl.obolibrary.org/obo/MAT_0000488 denotes later

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 599-620 Glycan denotes chondroitin 6-sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G34992DF
T2 1518-1529 Glycan denotes chondroitin https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 599-620 Glycan denotes chondroitin 6-sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G34992DF
T2 1518-1529 Glycan denotes chondroitin https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 599-620 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin 6-sulfate http://www.glycoepitope.jp/epitopes/EP0083
T2 1518-1529 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin http://www.glycoepitope.jp/epitopes/EP0081

GlyCosmos15-CL

Id Subject Object Predicate Lexical cue cl_id
T1 59-73 Cell denotes megakaryocytes http://purl.obolibrary.org/obo/CL:0000556
T2 78-87 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T3 110-123 Cell denotes megakaryocyte http://purl.obolibrary.org/obo/CL:0000556
T4 139-147 Cell denotes platelet http://purl.obolibrary.org/obo/CL:0000233
T5 225-238 Cell denotes megakaryocyte http://purl.obolibrary.org/obo/CL:0000556
T6 304-318 Cell denotes megakaryocytes http://purl.obolibrary.org/obo/CL:0000556
T7 323-332 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T8 852-861 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T9 1030-1043 Cell denotes megakaryocyte http://purl.obolibrary.org/obo/CL:0000556
T10 1161-1170 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T11 1211-1225 Cell denotes megakaryocytes http://purl.obolibrary.org/obo/CL:0000556
T12 1414-1423 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T13 1476-1490 Cell denotes megakaryocytes http://purl.obolibrary.org/obo/CL:0000556
T14 1647-1656 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 48-58 Organism denotes guinea pig 10141
T2 259-270 Organism denotes Guinea pigs 10140

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 59-73 Body_part denotes megakaryocytes http://purl.obolibrary.org/obo/CL_0000556
T2 78-87 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T3 110-123 Body_part denotes megakaryocyte http://purl.obolibrary.org/obo/CL_0000556
T4 139-147 Body_part denotes platelet http://purl.obolibrary.org/obo/CL_0000233
T5 225-238 Body_part denotes megakaryocyte http://purl.obolibrary.org/obo/CL_0000556
T6 304-318 Body_part denotes megakaryocytes http://purl.obolibrary.org/obo/CL_0000556
T7 323-332 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T8 852-861 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T9 1030-1043 Body_part denotes megakaryocyte http://purl.obolibrary.org/obo/CL_0000556
T10 1161-1170 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T11 1211-1225 Body_part denotes megakaryocytes http://purl.obolibrary.org/obo/CL_0000556
T12 1414-1423 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T13 1476-1490 Body_part denotes megakaryocytes http://purl.obolibrary.org/obo/CL_0000556
T14 1647-1656 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-96 Sentence denotes Sulfated proteoglycans and sulfated proteins in guinea pig megakaryocytes and platelets in vivo.
T2 97-159 Sentence denotes Relevance to megakaryocyte maturation and platelet activation.
T3 160-258 Sentence denotes This study has examined changes in proteoglycan synthesis during megakaryocyte maturation in vivo.
T4 259-372 Sentence denotes Guinea pigs were injected with Na235SO4, and megakaryocytes and platelets were isolated from 3 h to 5 days later.
T5 373-579 Sentence denotes The proteoglycans and other sulfated molecules in both cells were characterized at each time point by gel filtration, ion-exchange chromatography, gel electrophoresis, and chemical and enzymatic digestions.
T6 580-678 Sentence denotes Two populations of chondroitin 6-sulfate proteoglycans were found by DEAE-Sephacel chromatography.
T7 679-805 Sentence denotes The major fraction was eluted with 4 M guanidine hydrochloride and the minor fraction with 4 M guanidine HCl, 2% Triton X-100.
T8 806-1016 Sentence denotes The Kav of the major proteoglycan peak in the platelets at 1 day after injection was 0.18-0.20 on Sepharose CL-6B and decreased gradually to 0.12 by 3 days, when proteoglycan radioactivity per cell was maximal.
T9 1017-1095 Sentence denotes The peak for megakaryocyte proteoglycans at 3 h was broad, with Kav = 0.1-0.2.
T10 1096-1226 Sentence denotes The appearance of different portions of the proteoglycan peak in platelets coincided with their disappearance from megakaryocytes.
T11 1227-1294 Sentence denotes Proteoglycan size was a function of glycosaminoglycan chain length.
T12 1295-1453 Sentence denotes The proteoglycans eluted with Triton X-100 from DEAE-Sephacel (Kav = 0.04-0.07 on Sepharose CL-6B) were not labeled in platelets until 2 days after injection.
T13 1454-1587 Sentence denotes Our data suggest that megakaryocytes synthesize different-sized chondroitin sulfate proteoglycans at different stages of development.
T14 1588-1721 Sentence denotes The proteoglycans of the major fraction were released from platelets in response to thrombin, and a small amount was released by ADP.
T15 1722-1804 Sentence denotes The proteoglycans of the Triton X-100 eluate were not released by thrombin or ADP.
T16 1805-1953 Sentence denotes About 20% of the sulfate radioactivity was incorporated into molecules that appear to be sulfated proteins and were not released by thrombin or ADP.

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 599-620 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin 6-sulfate http://www.glycoepitope.jp/epitopes/EP0083
T2 1518-1529 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin http://www.glycoepitope.jp/epitopes/EP0081

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 59-73 Body_part denotes megakaryocytes FMA:83555
T2 78-87 Body_part denotes platelets FMA:62851
T3 110-123 Body_part denotes megakaryocyte FMA:83555
T4 139-147 Body_part denotes platelet FMA:62851
T5 225-238 Body_part denotes megakaryocyte FMA:83555
T6 304-318 Body_part denotes megakaryocytes FMA:83555
T7 323-332 Body_part denotes platelets FMA:62851
T8 852-861 Body_part denotes platelets FMA:62851
T9 1030-1043 Body_part denotes megakaryocyte FMA:83555
T10 1161-1170 Body_part denotes platelets FMA:62851
T11 1211-1225 Body_part denotes megakaryocytes FMA:83555
T12 1414-1423 Body_part denotes platelets FMA:62851
T13 1476-1490 Body_part denotes megakaryocytes FMA:83555
T14 1647-1656 Body_part denotes platelets FMA:62851

GlyCosmos15-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 366-371 Body_part denotes later http://purl.obolibrary.org/obo/MAT_0000488

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 48-58 OrganismTaxon denotes guinea pig 10141
T2 259-270 OrganismTaxon denotes Guinea pigs 10140

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 59-73 Body_part denotes megakaryocytes http://purl.obolibrary.org/obo/CL_0000556
T2 78-87 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T3 110-123 Body_part denotes megakaryocyte http://purl.obolibrary.org/obo/CL_0000556
T4 139-147 Body_part denotes platelet http://purl.obolibrary.org/obo/CL_0000233
T5 225-238 Body_part denotes megakaryocyte http://purl.obolibrary.org/obo/CL_0000556
T6 304-318 Body_part denotes megakaryocytes http://purl.obolibrary.org/obo/CL_0000556
T7 323-332 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T8 852-861 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T9 1030-1043 Body_part denotes megakaryocyte http://purl.obolibrary.org/obo/CL_0000556
T10 1161-1170 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T11 1211-1225 Body_part denotes megakaryocytes http://purl.obolibrary.org/obo/CL_0000556
T12 1414-1423 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233
T13 1476-1490 Body_part denotes megakaryocytes http://purl.obolibrary.org/obo/CL_0000556
T14 1647-1656 Body_part denotes platelets http://purl.obolibrary.org/obo/CL_0000233

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 366-371 Body_part denotes later http://purl.obolibrary.org/obo/MAT_0000488

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 59-73 Cell denotes megakaryocytes http://purl.obolibrary.org/obo/CL:0000556
T2 78-87 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T3 110-123 Cell denotes megakaryocyte http://purl.obolibrary.org/obo/CL:0000556
T4 139-147 Cell denotes platelet http://purl.obolibrary.org/obo/CL:0000233
T5 225-238 Cell denotes megakaryocyte http://purl.obolibrary.org/obo/CL:0000556
T6 304-318 Cell denotes megakaryocytes http://purl.obolibrary.org/obo/CL:0000556
T7 323-332 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T8 852-861 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T9 1030-1043 Cell denotes megakaryocyte http://purl.obolibrary.org/obo/CL:0000556
T10 1161-1170 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T11 1211-1225 Cell denotes megakaryocytes http://purl.obolibrary.org/obo/CL:0000556
T12 1414-1423 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233
T13 1476-1490 Cell denotes megakaryocytes http://purl.obolibrary.org/obo/CL:0000556
T14 1647-1656 Cell denotes platelets http://purl.obolibrary.org/obo/CL:0000233