| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-117 |
Sentence |
denotes |
Structure determination of Cryptococcus neoformans serotype A-variant glucuronoxylomannan by 13C-n.m.r. spectroscopy. |
| TextSentencer_T2 |
118-405 |
Sentence |
denotes |
A series of polysaccharides was derived by physical and chemical methods from an antigenic, O-acetyl-containing, glucuronoxylomannan (GXM), isolated from the growth medium of Cryptococcus neoformans (CDC B2550) serotype A-variant having composition ratios of Man:Xyl:GlcA:OAc = 10:4:3:6. |
| TextSentencer_T3 |
406-481 |
Sentence |
denotes |
13C-N.m.r. spectra of derivatives provided new structural evidence for GXM. |
| TextSentencer_T4 |
482-569 |
Sentence |
denotes |
Treatment of GXM with Li in ethylenediamine gave a xylomannan (XM, with Man:Xyl = 5:2). |
| TextSentencer_T5 |
570-612 |
Sentence |
denotes |
Smith degradation of XM gave a mannan (M). |
| TextSentencer_T6 |
613-667 |
Sentence |
denotes |
Ultrasonic treatment of GXM gave GXM-sonicated (GXMS). |
| TextSentencer_T7 |
668-856 |
Sentence |
denotes |
Treatment of GXM with 3-(3-dimethylaminopropyl)-1-ethylcarbodiimide.HCl and then with NaBH4 gave reduced GXMS (RGXMS), or with aq. trifluoroacetic acid gave partially acid-hydrolyzed GXMS. |
| TextSentencer_T8 |
857-950 |
Sentence |
denotes |
Periodate oxidation of GXM and NaBH4 reduction of the product gave a polyalcohol-mannan (PM). |
| TextSentencer_T9 |
951-1047 |
Sentence |
denotes |
Treatment of GXMS, RGXMS, and PM with NH4OH at pH 11 gave the respective O-deacetylated analogs. |
| TextSentencer_T10 |
1048-1185 |
Sentence |
denotes |
Comparison among the 13C-n.m.r. spectra of GXM, the various derivatives, and reference monosaccharides allowed the following conclusions: |
| TextSentencer_T11 |
1186-1393 |
Sentence |
denotes |
M is (1----3)-alpha-D-mannopyranan; XM consists of the M backbone with 91% of the Xyl on nonadjacent Man residues as 2-O-beta-D-Xylp substituents and with 9% as 4-O-D-Xylp substituents on other Man residues. |
| TextSentencer_T12 |
1394-1646 |
Sentence |
denotes |
GXM consists of the XM structure, but with non-D-xylosylated Man residues substituted with 2-O-beta-D-GlcpA substituents and with 6-O-acetyl groups distributed approximately equally on Man residues that have other substituents and those that have none. |
| TextSentencer_T13 |
1647-1790 |
Sentence |
denotes |
The molecular mechanics program MM2 was used to estimate the relative energies of anomeric orientations of the typical glycosidic linkage in M. |
| TextSentencer_T14 |
1791-2047 |
Sentence |
denotes |
The results suggest that 6'-OH----O-2 H-bonding is significant in the minimal-energy orientation of M, with phi = -36 degrees and psi = 51 degrees, and that two other glycosidic orientations may be important in the 2-O- or 6-O-substituted derivatives of M. |
| T1 |
0-117 |
Sentence |
denotes |
Structure determination of Cryptococcus neoformans serotype A-variant glucuronoxylomannan by 13C-n.m.r. spectroscopy. |
| T2 |
118-405 |
Sentence |
denotes |
A series of polysaccharides was derived by physical and chemical methods from an antigenic, O-acetyl-containing, glucuronoxylomannan (GXM), isolated from the growth medium of Cryptococcus neoformans (CDC B2550) serotype A-variant having composition ratios of Man:Xyl:GlcA:OAc = 10:4:3:6. |
| T3 |
406-481 |
Sentence |
denotes |
13C-N.m.r. spectra of derivatives provided new structural evidence for GXM. |
| T4 |
482-569 |
Sentence |
denotes |
Treatment of GXM with Li in ethylenediamine gave a xylomannan (XM, with Man:Xyl = 5:2). |
| T5 |
570-612 |
Sentence |
denotes |
Smith degradation of XM gave a mannan (M). |
| T6 |
613-667 |
Sentence |
denotes |
Ultrasonic treatment of GXM gave GXM-sonicated (GXMS). |
| T7 |
668-856 |
Sentence |
denotes |
Treatment of GXM with 3-(3-dimethylaminopropyl)-1-ethylcarbodiimide.HCl and then with NaBH4 gave reduced GXMS (RGXMS), or with aq. trifluoroacetic acid gave partially acid-hydrolyzed GXMS. |
| T8 |
857-950 |
Sentence |
denotes |
Periodate oxidation of GXM and NaBH4 reduction of the product gave a polyalcohol-mannan (PM). |
| T9 |
951-1047 |
Sentence |
denotes |
Treatment of GXMS, RGXMS, and PM with NH4OH at pH 11 gave the respective O-deacetylated analogs. |
| T10 |
1048-1185 |
Sentence |
denotes |
Comparison among the 13C-n.m.r. spectra of GXM, the various derivatives, and reference monosaccharides allowed the following conclusions: |
| T11 |
1186-1393 |
Sentence |
denotes |
M is (1----3)-alpha-D-mannopyranan; XM consists of the M backbone with 91% of the Xyl on nonadjacent Man residues as 2-O-beta-D-Xylp substituents and with 9% as 4-O-D-Xylp substituents on other Man residues. |
| T12 |
1394-1646 |
Sentence |
denotes |
GXM consists of the XM structure, but with non-D-xylosylated Man residues substituted with 2-O-beta-D-GlcpA substituents and with 6-O-acetyl groups distributed approximately equally on Man residues that have other substituents and those that have none. |
| T13 |
1647-1790 |
Sentence |
denotes |
The molecular mechanics program MM2 was used to estimate the relative energies of anomeric orientations of the typical glycosidic linkage in M. |
| T14 |
1791-2047 |
Sentence |
denotes |
The results suggest that 6'-OH----O-2 H-bonding is significant in the minimal-energy orientation of M, with phi = -36 degrees and psi = 51 degrees, and that two other glycosidic orientations may be important in the 2-O- or 6-O-substituted derivatives of M. |