PubMed:32508867
Annnotations
OryzaGP_2021_v2
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 896-901 | http://identifiers.org/oryzabase.gene/6771 | denotes | OsPDS |
| T2 | 903-909 | http://identifiers.org/oryzabase.gene/8503 | denotes | OsDEP1 |
| T3 | 914-920 | http://identifiers.org/oryzabase.gene/2866 | denotes | OsROC5 |
| T3640 | 896-901 | http://identifiers.org/rapdb.locus/Os03g0184000 | denotes | OsPDS |
| T33441 | 903-909 | http://identifiers.org/rapdb.locus/Os09g0441900 | denotes | OsDEP1 |
| T69594 | 914-920 | http://identifiers.org/rapdb.locus/Os02g0674800 | denotes | OsROC5 |
OryzaGP_2022
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 43-52 | http://identifiers.org/oryzabase.gene/17712 | denotes | Ubiquitin |
| T2 | 694-703 | http://identifiers.org/oryzabase.gene/17712 | denotes | ubiquitin |
OryzaGP_2021_FLAIR
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| M_0 | 791-799 | hunflair:NA:Gene | denotes | Cas9-UBA |
| M_1 | 1236-1244 | hunflair:NA:Gene | denotes | Cas9-UBA |
| M_2 | 694-703 | hunflair:NA:Gene | denotes | ubiquitin |
| M_3 | 914-920 | hunflair:NA:Gene | denotes | OsROC5 |
| M_4 | 92-97 | hunflair:NA:Species | denotes | Plant |
| M_5 | 19-30 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_6 | 155-166 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_7 | 351-362 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_8 | 542-553 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_9 | 1229-1240 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_10 | 1332-1343 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_11 | 43-52 | hunflair:NA:Gene | denotes | Ubiquitin |
| M_12 | 891-895 | hunflair:NA:Species | denotes | rice |
| M_13 | 984-988 | hunflair:NA:Species | denotes | rice |
| M_14 | 1024-1028 | hunflair:NA:Species | denotes | rice |
| M_15 | 903-909 | hunflair:NA:Gene | denotes | OsDEP1 |
| M_16 | 896-901 | hunflair:NA:Gene | denotes | OsPDS |
| M_17 | 26-30 | hunflair:NA:Gene | denotes | Cas9 |
| M_18 | 162-166 | hunflair:NA:Gene | denotes | Cas9 |
| M_19 | 358-362 | hunflair:NA:Gene | denotes | Cas9 |
| M_20 | 549-553 | hunflair:NA:Gene | denotes | Cas9 |
| M_21 | 756-760 | hunflair:NA:Gene | denotes | Cas9 |
| M_22 | 791-795 | hunflair:NA:Gene | denotes | Cas9 |
| M_23 | 1123-1127 | hunflair:NA:Gene | denotes | Cas9 |
| M_24 | 1236-1240 | hunflair:NA:Gene | denotes | Cas9 |
| M_25 | 1339-1343 | hunflair:NA:Gene | denotes | Cas9 |
OryzaGP_2021
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 43-52 | http://identifiers.org/oryzabase.gene/7107 | denotes | Ubiquitin |
| T2 | 43-52 | http://identifiers.org/oryzabase.gene/17712 | denotes | Ubiquitin |
| T3 | 694-703 | http://identifiers.org/oryzabase.gene/7107 | denotes | ubiquitin |
| T4 | 694-703 | http://identifiers.org/oryzabase.gene/17712 | denotes | ubiquitin |
| T5 | 896-901 | http://identifiers.org/oryzabase.gene/6771 | denotes | OsPDS |
| T6 | 903-909 | http://identifiers.org/oryzabase.gene/8503 | denotes | OsDEP1 |
| T7 | 914-920 | http://identifiers.org/oryzabase.gene/2866 | denotes | OsROC5 |
| T59658 | 43-52 | http://identifiers.org/ricegap/LOC_Os09g39500 | denotes | Ubiquitin |
| T70816 | 43-52 | http://identifiers.org/ricegap/LOC_Os06g44080 | denotes | Ubiquitin |
| T42854 | 694-703 | http://identifiers.org/ricegap/LOC_Os09g39500 | denotes | ubiquitin |
| T96078 | 694-703 | http://identifiers.org/ricegap/LOC_Os06g44080 | denotes | ubiquitin |
| T54121 | 896-901 | http://identifiers.org/ricegap/LOC_Os03g08570 | denotes | OsPDS |
| T27195 | 903-909 | http://identifiers.org/ricegap/LOC_Os09g26999 | denotes | OsDEP1 |
| T99350 | 914-920 | http://identifiers.org/ricegap/LOC_Os02g45250 | denotes | OsROC5 |
| T13680 | 43-52 | http://identifiers.org/rapdb.locus/Os09g0568400 | denotes | Ubiquitin |
| T86659 | 43-52 | http://identifiers.org/rapdb.locus/Os06g0650100 | denotes | Ubiquitin |
| T31117 | 694-703 | http://identifiers.org/rapdb.locus/Os09g0568400 | denotes | ubiquitin |
| T93295 | 694-703 | http://identifiers.org/rapdb.locus/Os06g0650100 | denotes | ubiquitin |
| T42607 | 896-901 | http://identifiers.org/rapdb.locus/Os03g0184000 | denotes | OsPDS |
| T33799 | 903-909 | http://identifiers.org/rapdb.locus/Os09g0441900 | denotes | OsDEP1 |
| T51629 | 914-920 | http://identifiers.org/rapdb.locus/Os02g0674800 | denotes | OsROC5 |
| T69968 | 43-52 | http://identifiers.org/uniprot/Q7XYD4 | denotes | Ubiquitin |
| T16032 | 43-52 | http://identifiers.org/uniprot/P0C031 | denotes | Ubiquitin |
| T69684 | 896-901 | http://identifiers.org/uniprot/Q0DUI8 | denotes | OsPDS |
| T66487 | 903-909 | http://identifiers.org/uniprot/Q67UU9 | denotes | OsDEP1 |
| T9366 | 903-909 | http://identifiers.org/uniprot/B9VI84 | denotes | OsDEP1 |
| T30577 | 903-909 | http://identifiers.org/uniprot/B8Y995 | denotes | OsDEP1 |
| T86335 | 914-920 | http://identifiers.org/uniprot/Q6EPF0 | denotes | OsROC5 |
| M_0 | 791-799 | hunflair:NA:Gene | denotes | Cas9-UBA |
| M_1 | 1236-1244 | hunflair:NA:Gene | denotes | Cas9-UBA |
| M_2 | 694-703 | hunflair:NA:Gene | denotes | ubiquitin |
| M_3 | 914-920 | hunflair:NA:Gene | denotes | OsROC5 |
| M_4 | 92-97 | hunflair:NA:Species | denotes | Plant |
| M_5 | 19-30 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_6 | 155-166 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_7 | 351-362 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_8 | 542-553 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_9 | 1229-1240 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_10 | 1332-1343 | hunflair:NA:Gene | denotes | CRISPR-Cas9 |
| M_11 | 43-52 | hunflair:NA:Gene | denotes | Ubiquitin |
| M_12 | 891-895 | hunflair:NA:Species | denotes | rice |
| M_13 | 984-988 | hunflair:NA:Species | denotes | rice |
| M_14 | 1024-1028 | hunflair:NA:Species | denotes | rice |
| M_15 | 903-909 | hunflair:NA:Gene | denotes | OsDEP1 |
| M_16 | 896-901 | hunflair:NA:Gene | denotes | OsPDS |
| M_17 | 26-30 | hunflair:NA:Gene | denotes | Cas9 |
| M_18 | 162-166 | hunflair:NA:Gene | denotes | Cas9 |
| M_19 | 358-362 | hunflair:NA:Gene | denotes | Cas9 |
| M_20 | 549-553 | hunflair:NA:Gene | denotes | Cas9 |
| M_21 | 756-760 | hunflair:NA:Gene | denotes | Cas9 |
| M_22 | 791-795 | hunflair:NA:Gene | denotes | Cas9 |
| M_23 | 1123-1127 | hunflair:NA:Gene | denotes | Cas9 |
| M_24 | 1236-1240 | hunflair:NA:Gene | denotes | Cas9 |
| M_25 | 1339-1343 | hunflair:NA:Gene | denotes | Cas9 |
Oryza_sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-113 | Sentence | denotes | The Improvement of CRISPR-Cas9 System With Ubiquitin-Associated Domain Fusion for Efficient Plant Genome Editing. |
| T2 | 114-281 | Sentence | denotes | Genome editing technology represented by CRISPR-Cas9 had been widely used in many biological fields such as gene function analysis, gene therapy, and crop improvement. |
| T3 | 282-475 | Sentence | denotes | However, in the face of the complexity of the eukaryotic genome, the CRISPR-Cas9 genome editing tools have shown an unstable editing efficiency with large variability at different target sites. |
| T4 | 476-584 | Sentence | denotes | It was important to further improve the editing efficiency of the CRISPR-Cas9 system among the whole genome. |
| T5 | 585-859 | Sentence | denotes | In this study, based on the previous single transcription unit genome editing system (STU-SpCas9), using the ubiquitin-associated domain (UBA) to enhance the stability of Cas9 protein, we constructed three Cas9-UBA fusion systems (SpCas9-SD01, SpCas9-SD02, and SpCas9-SD03). |
| T6 | 860-1036 | Sentence | denotes | Four different target sites of rice OsPDS, OsDEP1 and OsROC5 genes were chosen to evaluate the genome editing efficiency in rice protoplasts and stable transformed rice plants. |
| T7 | 1037-1219 | Sentence | denotes | The results showed that the fusion of UBA domains did not affect the cleavage mode of Cas9 protein, and effectively increase the editing efficiency of STU-SpCas9 at the target sites. |
| T8 | 1220-1354 | Sentence | denotes | This new CRISPR-Cas9-UBA system provided a new strategy and tool for improving the genome editing efficiency of CRISPR-Cas9 in plants. |