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Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-89 Sentence denotes PIK3R3 regulates ZO-1 expression through the NF-kB pathway in inflammatory bowel disease.
T2 90-110 Sentence denotes BACKGROUND AND AIMS:
T3 111-217 Sentence denotes Inflammatory bowel disease (IBD) are the major risk factor for developing colitis associated cancer (CAC).
T4 218-398 Sentence denotes Previously, we have reported that Phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) was overexpressed in colorectal cancer (CRC), but we don't know the role of PIK3R3 in IBD.
T5 399-407 Sentence denotes METHODS:
T6 408-574 Sentence denotes We investigated the differential expression of PIK3R3 and ZO-1 in IBD patients by using Immunohistochemical (IHC) and Gene Expression Omnibus (GEO) database analysis.
T7 575-676 Sentence denotes Caco-2 cells were exposed to different conditions to assess protein level changes of PIK3R3 and ZO-1.
T8 677-786 Sentence denotes Caco-2 cell monolayers were transfected with PIK3R3/siPIK3R3 to assess transepithelial electrical resistance.
T9 787-870 Sentence denotes Tight junction protein integrity was assessed by immunoblot and immunofluorescence.
T10 871-1048 Sentence denotes For further, intestinal permeability and tight junction protein integrity were assessed in animal study to assess the treatment role of PIK3R3 specific inhibitor TAT-N 15 (N15).
T11 1049-1145 Sentence denotes RESULTS: PIK3R3 was increased in IBD patients, and negatively controlled the expression of ZO-1.
T12 1146-1206 Sentence denotes In vitro, PIK3R3 regulates ZO-1 by activating NF-kB pathway.
T13 1207-1354 Sentence denotes Overexpression of PIK3R3 in Caco-2 cells decreased transepithelial electrical resistance (TEER), an opposite result was observed in siPIK3R3 cells.
T14 1355-1467 Sentence denotes In animal study, inhibition of PIK3R3 by N15 contributed to amelioration of DSS-induced intestinal permeability.
T15 1468-1540 Sentence denotes Mice treated with N15 exhibited less disruption of TJs in colon tissues.
T16 1541-1648 Sentence denotes CONCLUSIONS: PIK3R3 was increased in clinical IBD patients with accompanying disruption of ZO-1 expression.
T17 1649-1723 Sentence denotes Inhibition of PIK3R3 attenuated DSS-induced IBD symptoms in a mouse model.
T18 1724-1799 Sentence denotes These findings indicated that PIK3R3 could be a therapeutic target for IBD.
T1 0-89 Sentence denotes PIK3R3 regulates ZO-1 expression through the NF-kB pathway in inflammatory bowel disease.
T2 90-110 Sentence denotes BACKGROUND AND AIMS:
T3 111-217 Sentence denotes Inflammatory bowel disease (IBD) are the major risk factor for developing colitis associated cancer (CAC).
T4 218-398 Sentence denotes Previously, we have reported that Phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) was overexpressed in colorectal cancer (CRC), but we don't know the role of PIK3R3 in IBD.
T5 399-407 Sentence denotes METHODS:
T6 408-574 Sentence denotes We investigated the differential expression of PIK3R3 and ZO-1 in IBD patients by using Immunohistochemical (IHC) and Gene Expression Omnibus (GEO) database analysis.
T7 575-676 Sentence denotes Caco-2 cells were exposed to different conditions to assess protein level changes of PIK3R3 and ZO-1.
T8 677-786 Sentence denotes Caco-2 cell monolayers were transfected with PIK3R3/siPIK3R3 to assess transepithelial electrical resistance.
T9 787-870 Sentence denotes Tight junction protein integrity was assessed by immunoblot and immunofluorescence.
T10 871-1048 Sentence denotes For further, intestinal permeability and tight junction protein integrity were assessed in animal study to assess the treatment role of PIK3R3 specific inhibitor TAT-N 15 (N15).
T11 1049-1145 Sentence denotes RESULTS: PIK3R3 was increased in IBD patients, and negatively controlled the expression of ZO-1.
T12 1146-1206 Sentence denotes In vitro, PIK3R3 regulates ZO-1 by activating NF-kB pathway.
T13 1207-1354 Sentence denotes Overexpression of PIK3R3 in Caco-2 cells decreased transepithelial electrical resistance (TEER), an opposite result was observed in siPIK3R3 cells.
T14 1355-1467 Sentence denotes In animal study, inhibition of PIK3R3 by N15 contributed to amelioration of DSS-induced intestinal permeability.
T15 1468-1540 Sentence denotes Mice treated with N15 exhibited less disruption of TJs in colon tissues.
T16 1541-1648 Sentence denotes CONCLUSIONS: PIK3R3 was increased in clinical IBD patients with accompanying disruption of ZO-1 expression.
T17 1649-1723 Sentence denotes Inhibition of PIK3R3 attenuated DSS-induced IBD symptoms in a mouse model.
T18 1724-1799 Sentence denotes These findings indicated that PIK3R3 could be a therapeutic target for IBD.