PubMed:32414432 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/32414432","sourcedb":"PubMed","sourceid":"32414432","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/32414432","text":"Physically crosslinked silk fibroin/hyaluronic acid scaffolds.\nCombining the properties of natural protein and polysaccharide is a promising strategy to generate bioactive biomaterials with controlled structure. Here, a new method of preparing water-insoluble silk fibroin/hyaluronic acid (SF/HA) scaffolds with tunable performances using an all-aqueous process is reported. Freezing-induced assembly was used to form silk I crystallization in the SF/HA blends. Silk I crystallization enhanced the stability of SF/HA scaffolds in water by forming silk I crystal networks to entrap blended HA without chemical cross-linking. Increasing HA content significantly enhanced the flexibility and water binding capacity of porous scaffolds, but high amount of HA reduced the water-stability of porous scaffolds due to insufficient silk I crystal cross-links. The enzymatic degradation behavior of the SF/HA scaffolds was investigated, revealing that the regulation ability of HA in the SF scaffolds. This novel nonchemically cross-linked protein/polysaccharide scaffold may be useful for soft tissue engineering due to excellent biocompatibility and tunable performances.","tracks":[{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":36,"end":51},"obj":"Chemical:MESH:D006820"},{"id":"T2","span":{"begin":111,"end":125},"obj":"Chemical:MESH:D011134"},{"id":"T3","span":{"begin":244,"end":249},"obj":"Chemical:MESH:D014867"},{"id":"T4","span":{"begin":273,"end":288},"obj":"Chemical:MESH:D006820"},{"id":"T5","span":{"begin":530,"end":535},"obj":"Chemical:MESH:D014867"},{"id":"T6","span":{"begin":689,"end":694},"obj":"Chemical:MESH:D014867"},{"id":"T7","span":{"begin":767,"end":772},"obj":"Chemical:MESH:D014867"},{"id":"T8","span":{"begin":810,"end":849},"obj":"Disease:MESH:C566783"},{"id":"T9","span":{"begin":1038,"end":1052},"obj":"Chemical:MESH:D011134"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"}]},{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":36,"end":51},"obj":"Chemical:MESH:D006820"},{"id":"T2","span":{"begin":111,"end":125},"obj":"Chemical:MESH:D011134"},{"id":"T3","span":{"begin":244,"end":249},"obj":"Chemical:MESH:D014867"},{"id":"T4","span":{"begin":273,"end":288},"obj":"Chemical:MESH:D006820"},{"id":"T5","span":{"begin":530,"end":535},"obj":"Chemical:MESH:D014867"},{"id":"T6","span":{"begin":689,"end":694},"obj":"Chemical:MESH:D014867"},{"id":"T7","span":{"begin":767,"end":772},"obj":"Chemical:MESH:D014867"},{"id":"T8","span":{"begin":810,"end":849},"obj":"Disease:MESH:C566783"},{"id":"T9","span":{"begin":1038,"end":1052},"obj":"Chemical:MESH:D011134"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"silkwormbase","color":"#ecb093","default":true},{"id":"silkworm","color":"#9693ec"}]}]}}