PubMed:32324213 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/32324213","sourcedb":"PubMed","sourceid":"32324213","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/32324213","text":"Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham.\nVirus infection by the Bombyx mori nucleopolyhedrovirus (BmNPV) is the most severe disease in Thai sericultural practice of polyvoltine silkworms. Here, we characterized a newly isolated BmNPV isolated from the Mahasarakham province in Thailand (BmNPV-MSU). The purity and morphology of BmNPV-MSU were examined using light microscopy and scanning electron microscopy. The polyhedral inclusion bodies (PIBs) of BmNPV-MSU appeared in tetragonal, hexagonal, octagonal, and globular forms. The virions were both single and multiple embedded as observed by transmission electron microscopy. We also determined the virulence of BmNPV-MSU for six different Thai polyvoltine strains by LC50 and time to death after infection. The LC50 values of Nang Lai, NK04, and Sam Rong strains were 5.05-1.52 × 107 PIBs per ml and mortality peaked 7- to 8-d after inoculation. For Nang Noi, SP2, and RE05 strains the LC50 values were 7.91-1.82 × 106 PIBs/ml and mortality peaked 4-5 d after inoculation, thus having lower chance of survival to infection by BmNPV-MSU.","tracks":[{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":43,"end":54},"obj":"Species:7091"},{"id":"T10","span":{"begin":380,"end":385},"obj":"Species:271108"},{"id":"T11","span":{"begin":503,"end":508},"obj":"Species:271108"},{"id":"T12","span":{"begin":715,"end":720},"obj":"Species:271108"},{"id":"T13","span":{"begin":788,"end":793},"obj":"Disease:MESH:D003643"},{"id":"T14","span":{"begin":800,"end":809},"obj":"Disease:MESH:D007239"},{"id":"T15","span":{"begin":904,"end":913},"obj":"Disease:MESH:D003643"},{"id":"T17","span":{"begin":1035,"end":1044},"obj":"Disease:MESH:D003643"},{"id":"T18","span":{"begin":1117,"end":1126},"obj":"Disease:MESH:D007239"},{"id":"T19","span":{"begin":1130,"end":1135},"obj":"Species:271108"},{"id":"T2","span":{"begin":60,"end":65},"obj":"Species:271108"},{"id":"T3","span":{"begin":79,"end":91},"obj":"Chemical:-"},{"id":"T4","span":{"begin":93,"end":108},"obj":"Disease:MESH:D001102"},{"id":"T5","span":{"begin":116,"end":148},"obj":"Species:271108"},{"id":"T6","span":{"begin":150,"end":155},"obj":"Species:271108"},{"id":"T7","span":{"begin":229,"end":238},"obj":"Species:7091"},{"id":"T8","span":{"begin":280,"end":285},"obj":"Species:271108"},{"id":"T9","span":{"begin":339,"end":344},"obj":"Species:271108"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T10","pred":"source","obj":"silkwormbase"},{"subj":"T11","pred":"source","obj":"silkwormbase"},{"subj":"T12","pred":"source","obj":"silkwormbase"},{"subj":"T13","pred":"source","obj":"silkwormbase"},{"subj":"T14","pred":"source","obj":"silkwormbase"},{"subj":"T15","pred":"source","obj":"silkwormbase"},{"subj":"T17","pred":"source","obj":"silkwormbase"},{"subj":"T18","pred":"source","obj":"silkwormbase"},{"subj":"T19","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"}]},{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":43,"end":54},"obj":"Species:7091"},{"id":"T2","span":{"begin":60,"end":65},"obj":"Species:271108"},{"id":"T3","span":{"begin":79,"end":91},"obj":"Chemical:-"},{"id":"T4","span":{"begin":93,"end":108},"obj":"Disease:MESH:D001102"},{"id":"T5","span":{"begin":116,"end":148},"obj":"Species:271108"},{"id":"T6","span":{"begin":150,"end":155},"obj":"Species:271108"},{"id":"T7","span":{"begin":229,"end":238},"obj":"Species:7091"},{"id":"T8","span":{"begin":280,"end":285},"obj":"Species:271108"},{"id":"T9","span":{"begin":339,"end":344},"obj":"Species:271108"},{"id":"T10","span":{"begin":380,"end":385},"obj":"Species:271108"},{"id":"T11","span":{"begin":503,"end":508},"obj":"Species:271108"},{"id":"T12","span":{"begin":715,"end":720},"obj":"Species:271108"},{"id":"T13","span":{"begin":788,"end":793},"obj":"Disease:MESH:D003643"},{"id":"T14","span":{"begin":800,"end":809},"obj":"Disease:MESH:D007239"},{"id":"T15","span":{"begin":904,"end":913},"obj":"Disease:MESH:D003643"},{"id":"T16","span":{"begin":964,"end":967},"obj":"Gene:6668"},{"id":"T17","span":{"begin":1035,"end":1044},"obj":"Disease:MESH:D003643"},{"id":"T18","span":{"begin":1117,"end":1126},"obj":"Disease:MESH:D007239"},{"id":"T19","span":{"begin":1130,"end":1135},"obj":"Species:271108"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"},{"subj":"T10","pred":"source","obj":"silkworm"},{"subj":"T11","pred":"source","obj":"silkworm"},{"subj":"T12","pred":"source","obj":"silkworm"},{"subj":"T13","pred":"source","obj":"silkworm"},{"subj":"T14","pred":"source","obj":"silkworm"},{"subj":"T15","pred":"source","obj":"silkworm"},{"subj":"T16","pred":"source","obj":"silkworm"},{"subj":"T17","pred":"source","obj":"silkworm"},{"subj":"T18","pred":"source","obj":"silkworm"},{"subj":"T19","pred":"source","obj":"silkworm"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"silkwormbase","color":"#cc93ec","default":true},{"id":"silkworm","color":"#93ecb2"}]}]}}