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PubMed:32149347 JSONTXT

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CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 62-70 Cell denotes monocyte http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T3 74-84 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T5 166-178 Cell denotes immune cells http://purl.obolibrary.org/obo/CL:0000738
T6 261-272 Cell denotes macrophages http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T8 278-287 Cell denotes monocytic http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T10 536-544 Cell denotes monocyte http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T12 548-558 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T14 599-608 Cell denotes monocytes http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T16 723-734 Cell denotes granulocyte http://purl.obolibrary.org/obo/CL:0000094
T17 735-745 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T19 966-975 Cell denotes monocytes http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T21 980-991 Cell denotes macrophages http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T23 1400-1410 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T25 1568-1577 Cell denotes monocytes http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T27 1626-1634 Cell denotes monocyte http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T29 1638-1648 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T31 1811-1822 Cell denotes macrophages http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T33 2077-2087 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T35 2153-2161 Cell denotes monocyte http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T37 2165-2175 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T39 2293-2304 Cell denotes immune cell http://purl.obolibrary.org/obo/CL:0000738

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 1738-1745 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T2 1962-1968 Glycan_Motif denotes core 1 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00031MO
T3 2011-2017 Glycan_Motif denotes core 2 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00033MO

sentences

Id Subject Object Predicate Lexical cue
T1 0-96 Sentence denotes High-resolution longitudinal N- and O-glycoprofiling of human monocyte-to-macrophage transition.
T2 97-179 Sentence denotes Protein glycosylation impacts the development and function of innate immune cells.
T3 180-340 Sentence denotes The glycophenotypes and the glycan remodelling associated with the maturation of macrophages from monocytic precursor populations remain incompletely described.
T4 341-570 Sentence denotes Herein, label-free porous graphitised carbon-liquid chromatography-tandem mass spectrometry (PGC-LC-MS/MS) was employed to profile with high resolution the N- and O-glycome associated with human monocyte-to-macrophage transition.
T5 571-824 Sentence denotes Primary blood-derived CD14+ monocytes were differentiated ex vivo in the absence of strong anti- and proinflammatory stimuli using a conventional 7-day granulocyte-macrophage colony-stimulating factor differentiation protocol with longitudinal sampling.
T6 825-937 Sentence denotes Morphology and protein expression monitored by light microscopy and proteomics validated the maturation process.
T7 938-1238 Sentence denotes Glycomics demonstrated that monocytes and macrophages display similar N-glycome profiles, comprising predominantly paucimannosidic (Man1-3GlcNAc2Fuc0-1, 22.1-30.8%), oligomannosidic (Man5-9GlcNAc2, 29.8-35.7%) and α2,3/6-sialylated complex-type N-glycans with variable core fucosylation (27.6-39.1%).
T8 1239-1427 Sentence denotes Glycopeptide analysis validated conjugation of these glycans to human proteins, while quantitative proteomics monitored the glycoenzyme expression levels during macrophage differentiation.
T9 1428-1578 Sentence denotes Significant interperson glycome variations were observed suggesting a considerable physiology-dependent or heritable heterogeneity of CD14+ monocytes.
T10 1579-1938 Sentence denotes Only few N-glycome changes correlated with the monocyte-to-macrophage transition across donors including decreased core fucosylation and reduced expression of mannose-terminating (paucimannosidic-/oligomannosidic-type) N-glycans in macrophages, while lectin flow cytometry indicated that more dramatic cell surface glycan remodelling occurs during maturation.
T11 1939-2099 Sentence denotes The less heterogeneous core 1-rich O-glycome showed a minor decrease in core 2-type O-glycosylation but otherwise remained unchanged with macrophage maturation.
T12 2100-2382 Sentence denotes This high-resolution glycome map underpinning normal monocyte-to-macrophage transition, the most detailed to date, aids our understanding of the molecular makeup pertaining to two vital innate immune cell types and forms an important reference for future glycoimmunological studies.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 1738-1745 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T2 1962-1968 https://glytoucan.org/Structures/Glycans/G00031MO denotes core 1

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 579-584 http://purl.obolibrary.org/obo/MAT_0000083 denotes blood
T2 579-584 http://purl.obolibrary.org/obo/MAT_0000315 denotes blood

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 1962-1968 Glycan denotes core 1 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00031MO
T2 2011-2017 Glycan denotes core 2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00033MO

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 56-61 OrganismTaxon denotes human 9606
T2 530-535 OrganismTaxon denotes human 9606
T3 1303-1308 OrganismTaxon denotes human 9606

GlyCosmos15-CL

Id Subject Object Predicate Lexical cue cl_id
T1 62-70 Cell denotes monocyte http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T3 74-84 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T5 166-178 Cell denotes immune cells http://purl.obolibrary.org/obo/CL:0000738
T6 261-272 Cell denotes macrophages http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T8 278-287 Cell denotes monocytic http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T10 536-544 Cell denotes monocyte http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T12 548-558 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T14 599-608 Cell denotes monocytes http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T16 723-734 Cell denotes granulocyte http://purl.obolibrary.org/obo/CL:0000094
T17 735-745 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T19 966-975 Cell denotes monocytes http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T21 980-991 Cell denotes macrophages http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T23 1400-1410 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T25 1568-1577 Cell denotes monocytes http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T27 1626-1634 Cell denotes monocyte http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T29 1638-1648 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T31 1811-1822 Cell denotes macrophages http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T33 2077-2087 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T35 2153-2161 Cell denotes monocyte http://purl.obolibrary.org/obo/CL:0000576|http://purl.obolibrary.org/obo/CL:0001054
T37 2165-2175 Cell denotes macrophage http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T39 2293-2304 Cell denotes immune cell http://purl.obolibrary.org/obo/CL:0000738

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 62-70 Body_part denotes monocyte http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T3 74-84 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T4 166-178 Body_part denotes immune cells http://purl.obolibrary.org/obo/CL_0000738
T5 261-272 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235
T6 536-544 Body_part denotes monocyte http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T8 548-558 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T9 579-584 Body_part denotes blood http://purl.obolibrary.org/obo/UBERON_0000178
T10 599-608 Body_part denotes monocytes http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T12 723-734 Body_part denotes granulocyte http://purl.obolibrary.org/obo/CL_0000094
T13 735-745 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T14 966-975 Body_part denotes monocytes http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T16 980-991 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235
T17 1400-1410 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T18 1568-1577 Body_part denotes monocytes http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T20 1626-1634 Body_part denotes monocyte http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T22 1638-1648 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T23 1811-1822 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235
T24 2077-2087 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T25 2153-2161 Body_part denotes monocyte http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T27 2165-2175 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T28 2293-2304 Body_part denotes immune cell http://purl.obolibrary.org/obo/CL_0000738

GlyCosmos15-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 579-584 Body_part denotes blood http://purl.obolibrary.org/obo/MAT_0000083|http://purl.obolibrary.org/obo/MAT_0000315

sentences

Id Subject Object Predicate Lexical cue
T1 0-96 Sentence denotes High-resolution longitudinal N- and O-glycoprofiling of human monocyte-to-macrophage transition.
T2 97-179 Sentence denotes Protein glycosylation impacts the development and function of innate immune cells.
T3 180-340 Sentence denotes The glycophenotypes and the glycan remodelling associated with the maturation of macrophages from monocytic precursor populations remain incompletely described.
T4 341-570 Sentence denotes Herein, label-free porous graphitised carbon-liquid chromatography-tandem mass spectrometry (PGC-LC-MS/MS) was employed to profile with high resolution the N- and O-glycome associated with human monocyte-to-macrophage transition.
T5 571-824 Sentence denotes Primary blood-derived CD14+ monocytes were differentiated ex vivo in the absence of strong anti- and proinflammatory stimuli using a conventional 7-day granulocyte-macrophage colony-stimulating factor differentiation protocol with longitudinal sampling.
T6 825-937 Sentence denotes Morphology and protein expression monitored by light microscopy and proteomics validated the maturation process.
T7 938-1238 Sentence denotes Glycomics demonstrated that monocytes and macrophages display similar N-glycome profiles, comprising predominantly paucimannosidic (Man1-3GlcNAc2Fuc0-1, 22.1-30.8%), oligomannosidic (Man5-9GlcNAc2, 29.8-35.7%) and α2,3/6-sialylated complex-type N-glycans with variable core fucosylation (27.6-39.1%).
T8 1239-1427 Sentence denotes Glycopeptide analysis validated conjugation of these glycans to human proteins, while quantitative proteomics monitored the glycoenzyme expression levels during macrophage differentiation.
T9 1428-1578 Sentence denotes Significant interperson glycome variations were observed suggesting a considerable physiology-dependent or heritable heterogeneity of CD14+ monocytes.
T10 1579-1938 Sentence denotes Only few N-glycome changes correlated with the monocyte-to-macrophage transition across donors including decreased core fucosylation and reduced expression of mannose-terminating (paucimannosidic-/oligomannosidic-type) N-glycans in macrophages, while lectin flow cytometry indicated that more dramatic cell surface glycan remodelling occurs during maturation.
T11 1939-2099 Sentence denotes The less heterogeneous core 1-rich O-glycome showed a minor decrease in core 2-type O-glycosylation but otherwise remained unchanged with macrophage maturation.
T12 2100-2382 Sentence denotes This high-resolution glycome map underpinning normal monocyte-to-macrophage transition, the most detailed to date, aids our understanding of the molecular makeup pertaining to two vital innate immune cell types and forms an important reference for future glycoimmunological studies.

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-96 Sentence denotes High-resolution longitudinal N- and O-glycoprofiling of human monocyte-to-macrophage transition.
T2 97-179 Sentence denotes Protein glycosylation impacts the development and function of innate immune cells.
T3 180-340 Sentence denotes The glycophenotypes and the glycan remodelling associated with the maturation of macrophages from monocytic precursor populations remain incompletely described.
T4 341-570 Sentence denotes Herein, label-free porous graphitised carbon-liquid chromatography-tandem mass spectrometry (PGC-LC-MS/MS) was employed to profile with high resolution the N- and O-glycome associated with human monocyte-to-macrophage transition.
T5 571-824 Sentence denotes Primary blood-derived CD14+ monocytes were differentiated ex vivo in the absence of strong anti- and proinflammatory stimuli using a conventional 7-day granulocyte-macrophage colony-stimulating factor differentiation protocol with longitudinal sampling.
T6 825-937 Sentence denotes Morphology and protein expression monitored by light microscopy and proteomics validated the maturation process.
T7 938-1238 Sentence denotes Glycomics demonstrated that monocytes and macrophages display similar N-glycome profiles, comprising predominantly paucimannosidic (Man1-3GlcNAc2Fuc0-1, 22.1-30.8%), oligomannosidic (Man5-9GlcNAc2, 29.8-35.7%) and α2,3/6-sialylated complex-type N-glycans with variable core fucosylation (27.6-39.1%).
T8 1239-1427 Sentence denotes Glycopeptide analysis validated conjugation of these glycans to human proteins, while quantitative proteomics monitored the glycoenzyme expression levels during macrophage differentiation.
T9 1428-1578 Sentence denotes Significant interperson glycome variations were observed suggesting a considerable physiology-dependent or heritable heterogeneity of CD14+ monocytes.
T10 1579-1938 Sentence denotes Only few N-glycome changes correlated with the monocyte-to-macrophage transition across donors including decreased core fucosylation and reduced expression of mannose-terminating (paucimannosidic-/oligomannosidic-type) N-glycans in macrophages, while lectin flow cytometry indicated that more dramatic cell surface glycan remodelling occurs during maturation.
T11 1939-2099 Sentence denotes The less heterogeneous core 1-rich O-glycome showed a minor decrease in core 2-type O-glycosylation but otherwise remained unchanged with macrophage maturation.
T12 2100-2382 Sentence denotes This high-resolution glycome map underpinning normal monocyte-to-macrophage transition, the most detailed to date, aids our understanding of the molecular makeup pertaining to two vital innate immune cell types and forms an important reference for future glycoimmunological studies.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 1962-1968 Glycan denotes core 1 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00031MO
T2 2011-2017 Glycan denotes core 2 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00033MO

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 56-61 OrganismTaxon denotes human 9606
T2 530-535 OrganismTaxon denotes human 9606
T3 1303-1308 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 62-70 Body_part denotes monocyte http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T3 74-84 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T4 166-178 Body_part denotes immune cells http://purl.obolibrary.org/obo/CL_0000738
T5 261-272 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235
T6 536-544 Body_part denotes monocyte http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T8 548-558 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T9 579-584 Body_part denotes blood http://purl.obolibrary.org/obo/UBERON_0000178
T10 599-608 Body_part denotes monocytes http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T12 723-734 Body_part denotes granulocyte http://purl.obolibrary.org/obo/CL_0000094
T13 735-745 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T14 966-975 Body_part denotes monocytes http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T16 980-991 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235
T17 1400-1410 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T18 1568-1577 Body_part denotes monocytes http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T20 1626-1634 Body_part denotes monocyte http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T22 1638-1648 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T23 1811-1822 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235
T24 2077-2087 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T25 2153-2161 Body_part denotes monocyte http://purl.obolibrary.org/obo/CL_0000576|http://purl.obolibrary.org/obo/CL_0001054
T27 2165-2175 Body_part denotes macrophage http://purl.obolibrary.org/obo/CL_0000235
T28 2293-2304 Body_part denotes immune cell http://purl.obolibrary.org/obo/CL_0000738

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 579-584 Body_part denotes blood http://purl.obolibrary.org/obo/MAT_0000083|http://purl.obolibrary.org/obo/MAT_0000315