| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-133 |
Sentence |
denotes |
Rational Design of Mechanism-Based Inhibitors and Activity-Based Probes for the Identification of Retaining α-l-Arabinofuranosidases. |
| T2 |
134-348 |
Sentence |
denotes |
Identifying and characterizing the enzymes responsible for an observed activity within a complex eukaryotic catabolic system remains one of the most significant challenges in the study of biomass-degrading systems. |
| T3 |
349-545 |
Sentence |
denotes |
The debranching of both complex hemicellulosic and pectinaceous polysaccharides requires the production of α-l-arabinofuranosidases among a wide variety of coexpressed carbohydrate-active enzymes. |
| T4 |
546-738 |
Sentence |
denotes |
To selectively detect and identify α-l-arabinofuranosidases produced by fungi grown on complex biomass, potential covalent inhibitors and probes which mimic α-l-arabinofuranosides were sought. |
| T5 |
739-899 |
Sentence |
denotes |
The conformational free energy landscapes of free α-l-arabinofuranose and several rationally designed covalent α-l-arabinofuranosidase inhibitors were analyzed. |
| T6 |
900-1007 |
Sentence |
denotes |
A synthetic route to these inhibitors was subsequently developed based on a key Wittig-Still rearrangement. |
| T7 |
1008-1244 |
Sentence |
denotes |
Through a combination of kinetic measurements, intact mass spectrometry, and structural experiments, the designed inhibitors were shown to efficiently label the catalytic nucleophiles of retaining GH51 and GH54 α-l-arabinofuranosidases. |
| T8 |
1245-1457 |
Sentence |
denotes |
Activity-based probes elaborated from an inhibitor with an aziridine warhead were applied to the identification and characterization of α-l-arabinofuranosidases within the secretome of A. niger grown on arabinan. |
| T9 |
1458-1628 |
Sentence |
denotes |
This method was extended to the detection and identification of α-l-arabinofuranosidases produced by eight biomass-degrading basidiomycete fungi grown on complex biomass. |
| T10 |
1629-1907 |
Sentence |
denotes |
The broad applicability of the cyclophellitol-derived activity-based probes and inhibitors presented here make them a valuable new tool in the characterization of complex eukaryotic carbohydrate-degrading systems and in the high-throughput discovery of α-l-arabinofuranosidases. |
