PubMed:31974493
Annnotations
OryzaGP_2021_v2
{"project":"OryzaGP_2021_v2","denotations":[{"id":"T1","span":{"begin":327,"end":330},"obj":"http://identifiers.org/oryzabase.gene/14686"},{"id":"T2","span":{"begin":387,"end":390},"obj":"http://identifiers.org/oryzabase.gene/14686"},{"id":"T3","span":{"begin":753,"end":756},"obj":"http://identifiers.org/oryzabase.gene/14686"},{"id":"T4","span":{"begin":840,"end":845},"obj":"http://identifiers.org/oryzabase.gene/6085"},{"id":"T5","span":{"begin":962,"end":968},"obj":"http://identifiers.org/oryzabase.gene/63"},{"id":"T56500","span":{"begin":327,"end":330},"obj":"http://identifiers.org/rapdb.locus/Os03g0731900"},{"id":"T38887","span":{"begin":387,"end":390},"obj":"http://identifiers.org/rapdb.locus/Os03g0731900"},{"id":"T52256","span":{"begin":753,"end":756},"obj":"http://identifiers.org/rapdb.locus/Os03g0731900"},{"id":"T8592","span":{"begin":840,"end":845},"obj":"http://identifiers.org/rapdb.locus/Os02g0510200"},{"id":"T83050","span":{"begin":962,"end":968},"obj":"http://identifiers.org/rapdb.locus/Os04g0550600"}],"text":"Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice.\nPrecise genome editing by systems such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) requires high-efficiency homology-directed repair (HDR). Different technologies have been developed to improve HDR but with limited success. Here, we generated a fusion between the Cas9 endonuclease and the Agrobacterium VirD2 relaxase (Cas9-VirD2). This chimeric protein combines the functions of Cas9, which produces targeted and specific DNA double-strand breaks (DSBs), and the VirD2 relaxase, which brings the repair template in close proximity to the DSBs, to facilitate HDR. We successfully employed our Cas9-VirD2 system for precise ACETOLACTATE SYNTHASE (OsALS) allele modification to generate herbicide-resistant rice (Oryza sativa) plants, CAROTENOID CLEAVAGE DIOXYGENASE-7 (OsCCD7) to engineer plant architecture, and generate in-frame fusions with the HA epitope at HISTONE DEACETYLASE (OsHDT) locus. The Cas9-VirD2 system expands our ability to improve agriculturally important traits in crops and opens new possibilities for precision genome engineering across diverse eukaryotic species."}
OryzaGP_2022
{"project":"OryzaGP_2022","denotations":[{"id":"T1","span":{"begin":817,"end":838},"obj":"http://identifiers.org/oryzabase.gene/6085"}],"text":"Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice.\nPrecise genome editing by systems such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) requires high-efficiency homology-directed repair (HDR). Different technologies have been developed to improve HDR but with limited success. Here, we generated a fusion between the Cas9 endonuclease and the Agrobacterium VirD2 relaxase (Cas9-VirD2). This chimeric protein combines the functions of Cas9, which produces targeted and specific DNA double-strand breaks (DSBs), and the VirD2 relaxase, which brings the repair template in close proximity to the DSBs, to facilitate HDR. We successfully employed our Cas9-VirD2 system for precise ACETOLACTATE SYNTHASE (OsALS) allele modification to generate herbicide-resistant rice (Oryza sativa) plants, CAROTENOID CLEAVAGE DIOXYGENASE-7 (OsCCD7) to engineer plant architecture, and generate in-frame fusions with the HA epitope at HISTONE DEACETYLASE (OsHDT) locus. The Cas9-VirD2 system expands our ability to improve agriculturally important traits in crops and opens new possibilities for precision genome engineering across diverse eukaryotic species."}
OryzaGP_2021_FLAIR
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OryzaGP_2021
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of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice.\nPrecise genome editing by systems such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) requires high-efficiency homology-directed repair (HDR). Different technologies have been developed to improve HDR but with limited success. Here, we generated a fusion between the Cas9 endonuclease and the Agrobacterium VirD2 relaxase (Cas9-VirD2). This chimeric protein combines the functions of Cas9, which produces targeted and specific DNA double-strand breaks (DSBs), and the VirD2 relaxase, which brings the repair template in close proximity to the DSBs, to facilitate HDR. We successfully employed our Cas9-VirD2 system for precise ACETOLACTATE SYNTHASE (OsALS) allele modification to generate herbicide-resistant rice (Oryza sativa) plants, CAROTENOID CLEAVAGE DIOXYGENASE-7 (OsCCD7) to engineer plant architecture, and generate in-frame fusions with the HA epitope at HISTONE DEACETYLASE (OsHDT) locus. The Cas9-VirD2 system expands our ability to improve agriculturally important traits in crops and opens new possibilities for precision genome engineering across diverse eukaryotic species."}