PubMed:31800874 JSONTXT

{"project":"sonoma2","denotations":[{"id":"T0","span":{"begin":70,"end":83},"obj":"DISEASE"},{"id":"T1","span":{"begin":178,"end":184},"obj":"REG"},{"id":"T2","span":{"begin":229,"end":247},"obj":"DISEASE"},{"id":"T3","span":{"begin":230,"end":248},"obj":"CPA"},{"id":"T4","span":{"begin":249,"end":252},"obj":"DISEASE"},{"id":"T5","span":{"begin":250,"end":253},"obj":"CPA"},{"id":"T6","span":{"begin":273,"end":279},"obj":"REG"},{"id":"T7","span":{"begin":282,"end":315},"obj":"GENE"},{"id":"T8","span":{"begin":283,"end":310},"obj":"GENE"},{"id":"T9","span":{"begin":301,"end":316},"obj":"GENE"},{"id":"T10","span":{"begin":317,"end":328},"obj":"GENE"},{"id":"T11","span":{"begin":318,"end":325},"obj":"GENE"},{"id":"T12","span":{"begin":334,"end":368},"obj":"GENE"},{"id":"T13","span":{"begin":353,"end":365},"obj":"GENE"},{"id":"T14","span":{"begin":369,"end":385},"obj":"GENE"},{"id":"T15","span":{"begin":451,"end":464},"obj":"DISEASE"},{"id":"T16","span":{"begin":454,"end":467},"obj":"DISEASE"},{"id":"T17","span":{"begin":656,"end":669},"obj":"DISEASE"},{"id":"T18","span":{"begin":658,"end":671},"obj":"DISEASE"},{"id":"T19","span":{"begin":674,"end":681},"obj":"REG"},{"id":"T20","span":{"begin":1172,"end":1175},"obj":"CPA"},{"id":"T21","span":{"begin":1173,"end":1176},"obj":"DISEASE"},{"id":"T22","span":{"begin":1194,"end":1199},"obj":"NEGREG"},{"id":"T23","span":{"begin":1245,"end":1252},"obj":"GENE"},{"id":"T24","span":{"begin":1246,"end":1257},"obj":"GENE"},{"id":"T25","span":{"begin":1287,"end":1292},"obj":"NEGREG"},{"id":"T26","span":{"begin":1334,"end":1354},"obj":"CPA"},{"id":"T27","span":{"begin":1339,"end":1353},"obj":"GENE"},{"id":"T28","span":{"begin":1374,"end":1379},"obj":"NEGREG"},{"id":"T29","span":{"begin":1512,"end":1521},"obj":"NEGREG"},{"id":"T30","span":{"begin":1522,"end":1548},"obj":"CPA"},{"id":"T31","span":{"begin":1526,"end":1540},"obj":"GENE"},{"id":"T32","span":{"begin":1565,"end":1574},"obj":"NEGREG"},{"id":"T33","span":{"begin":1575,"end":1582},"obj":"GENE"},{"id":"T34","span":{"begin":1579,"end":1590},"obj":"GENE"},{"id":"T35","span":{"begin":1641,"end":1645},"obj":"POSREG"},{"id":"T36","span":{"begin":1659,"end":1667},"obj":"NEGREG"},{"id":"T37","span":{"begin":1668,"end":1671},"obj":"CPA"},{"id":"T38","span":{"begin":1676,"end":1679},"obj":"DISEASE"}],"relations":[{"id":"R0","pred":"ThemeOf","subj":"T3","obj":"T1"},{"id":"R1","pred":"ThemeOf","subj":"T8","obj":"T6"},{"id":"R2","pred":"ThemeOf","subj":"T9","obj":"T6"},{"id":"R3","pred":"ThemeOf","subj":"T11","obj":"T6"},{"id":"R4","pred":"ThemeOf","subj":"T13","obj":"T6"},{"id":"R5","pred":"ThemeOf","subj":"T15","obj":"T6"},{"id":"R6","pred":"ThemeOf","subj":"T17","obj":"T19"},{"id":"R7","pred":"ThemeOf","subj":"T20","obj":"T22"},{"id":"R8","pred":"ThemeOf","subj":"T26","obj":"T28"},{"id":"R9","pred":"ThemeOf","subj":"T30","obj":"T29"},{"id":"R10","pred":"CauseOf","subj":"T35","obj":"T36"},{"id":"R11","pred":"ThemeOf","subj":"T37","obj":"T36"}],"text":"Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats.\nOBJECTIVE: The goal of the present study was to determine the effect of systemic and topical ozone application on alveolar bone loss (ABL) by evaluating the effect of Hypoxia-inducible factor -1 alpha (HIF-1-α) and receptor activator of NF-kB ligand (RANKL)-positive cells on histopathological and immunohistochemical changes in a rat periodontitis model.\nMETHODOLOGY: Thirty male Wistar rats were divided into three groups: 1) Group C (control group); 2) Group SO (systemic ozone group) and 3) Group TO (topical ozone group). Experimental periodontitis was induced with a 3/0 silk suture placed at the mandibular left first molars of rats, and the suture was removed 14 days later. Ozone gas was injected intraperitoneally (0.7 mg/kg) in SO group. Topical ozone application protocol was performed using an ozone generator at 80% concentration (4th grade) 90- degree probe for the duration of 30 s. Both ozone applications were carried out for two weeks at intervals of two days. Histomorphometric and immunohistochemical analysis were performed.\nRESULTS: ABL was significantly lower in Group SO compared to Group C (p: 0.0052). HIF-1α- positive cells were significantly lower in Group TO than in Group C (p: 0.0043). RANKL-positive cells were significantly lower in Group SO and in Group TO compared to the control group (p: 0.0033, p: 0.0075, respectively).\nCONCLUSION: Both ozone applications decreased RANKL-positive cell counts, TO application decreased HIF-1-α positive cells counts, and SO application was found to be more effective in reducing ABL compared to control group."}