PubMed:31574230 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/31574230","sourcedb":"PubMed","sourceid":"31574230","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/31574230","text":"Production of Brucella melitensis Omp16 protein fused to the human interleukin 2 in Lactococcus lactis MG1363 toward developing a Lactococcus-based vaccine against brucellosis.\nThe use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with appropriate restriction sites and subcloned in pAMJ2008 expression vector digested with the same enzymes. The purified recombinant constructed pAMJ-rOmp-IL2 was introduced into L. lactis subsp. cremoris MG1363 by electrotransformation. Finally, the expression and purification of Omp16-IL2 fusion protein was investigated. This study reports the construction of a recombinant L. lactis expressing the Omp16-IL2 fusion protein as an oral Lactococcus-based vaccine, as compared with commonly used live attenuated vaccines, for future studies against brucellosis.","tracks":[{"project":"sonoma2","denotations":[{"id":"T0","span":{"begin":0,"end":10},"obj":"MPA"},{"id":"T1","span":{"begin":34,"end":39},"obj":"GENE"},{"id":"T2","span":{"begin":67,"end":80},"obj":"GENE"},{"id":"T3","span":{"begin":67,"end":80},"obj":"GENE"},{"id":"T4","span":{"begin":117,"end":127},"obj":"REG"},{"id":"T5","span":{"begin":162,"end":173},"obj":"DISEASE"},{"id":"T6","span":{"begin":164,"end":175},"obj":"DISEASE"},{"id":"T7","span":{"begin":336,"end":345},"obj":"GENE"},{"id":"T8","span":{"begin":381,"end":390},"obj":"MPA"},{"id":"T9","span":{"begin":424,"end":433},"obj":"GENE"},{"id":"T10","span":{"begin":651,"end":661},"obj":"REG"},{"id":"T11","span":{"begin":739,"end":749},"obj":"MPA"},{"id":"T12","span":{"begin":754,"end":766},"obj":"MPA"},{"id":"T13","span":{"begin":770,"end":779},"obj":"GENE"},{"id":"T14","span":{"begin":836,"end":848},"obj":"REG"},{"id":"T15","span":{"begin":876,"end":886},"obj":"REG"},{"id":"T16","span":{"begin":891,"end":900},"obj":"GENE"},{"id":"T17","span":{"begin":1038,"end":1049},"obj":"DISEASE"}],"relations":[{"id":"R0","pred":"ThemeOf","subj":"T1","obj":"T0"},{"id":"R1","pred":"ThemeOf","subj":"T2","obj":"T0"},{"id":"R2","pred":"ThemeOf","subj":"T6","obj":"T4"},{"id":"R3","pred":"ThemeOf","subj":"T13","obj":"T12"},{"id":"R4","pred":"ThemeOf","subj":"T16","obj":"T14"},{"id":"R5","pred":"ThemeOf","subj":"T16","obj":"T15"},{"id":"R6","pred":"ThemeOf","subj":"T17","obj":"T15"}],"attributes":[{"subj":"T0","pred":"source","obj":"sonoma2"},{"subj":"T1","pred":"source","obj":"sonoma2"},{"subj":"T2","pred":"source","obj":"sonoma2"},{"subj":"T3","pred":"source","obj":"sonoma2"},{"subj":"T4","pred":"source","obj":"sonoma2"},{"subj":"T5","pred":"source","obj":"sonoma2"},{"subj":"T6","pred":"source","obj":"sonoma2"},{"subj":"T7","pred":"source","obj":"sonoma2"},{"subj":"T8","pred":"source","obj":"sonoma2"},{"subj":"T9","pred":"source","obj":"sonoma2"},{"subj":"T10","pred":"source","obj":"sonoma2"},{"subj":"T11","pred":"source","obj":"sonoma2"},{"subj":"T12","pred":"source","obj":"sonoma2"},{"subj":"T13","pred":"source","obj":"sonoma2"},{"subj":"T14","pred":"source","obj":"sonoma2"},{"subj":"T15","pred":"source","obj":"sonoma2"},{"subj":"T16","pred":"source","obj":"sonoma2"},{"subj":"T17","pred":"source","obj":"sonoma2"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sonoma2","color":"#95ec93","default":true}]}]}}