PubMed:31562344 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/31562344","sourcedb":"PubMed","sourceid":"31562344","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/31562344","text":"Distinct classes and subclasses of antibodies to hemolysin co-regulated protein 1 and O-polysaccharide and correlation with clinical characteristics of melioidosis patients.\nMelioidosis is a tropical infectious disease caused by Burkholderia pseudomallei that results in high mortality. Hemolysin co-regulated protein 1 (Hcp1) and O-polysaccharide (OPS) are vaccine candidates and potential diagnostic antigens. The correlation of classes/subclasses of antibodies against these antigens with clinical characteristics of melioidosis patients is unknown. Antibodies in plasma samples from melioidosis patients and healthy donors were quantified by ELISA and compared with clinical features. In melioidosis patients, Hcp1 induced high IgG levels. OPS induced high IgG and IgA levels. The area under receiver operating characteristic curve (AUROCC) to discriminate melioidosis cases from healthy donors was highest for anti-Hcp1 IgG (0.92) compared to anti-Hcp1 IgA or IgM. In contrast, AUROCC for anti-OPS for IgG (0.91) and IgA (0.92) were comparable. Anti-Hcp1 IgG1 and anti-OPS IgG2 had the greatest AUROCCs (0.87 and 0.95, respectively) compared to other IgG subclasses for each antigen. Survivors had significantly higher anti-Hcp1 IgG3 levels than non-survivors. Male melioidosis patients with diabetes had higher anti-OPS IgA levels than males without diabetes. Thus, diverse and specific antibody responses are associated with distinct clinical characteristics in melioidosis, confirming the diagnostic utility of these responses and providing new insights into immune mechanisms.","tracks":[{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":229,"end":254},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"28450"},{"subj":"T1","pred":"source","obj":"NCBITAXON"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":152,"end":163},"obj":"Disease"},{"id":"T2","span":{"begin":174,"end":185},"obj":"Disease"},{"id":"T3","span":{"begin":200,"end":218},"obj":"Disease"},{"id":"T4","span":{"begin":520,"end":531},"obj":"Disease"},{"id":"T5","span":{"begin":587,"end":598},"obj":"Disease"},{"id":"T6","span":{"begin":692,"end":703},"obj":"Disease"},{"id":"T7","span":{"begin":861,"end":872},"obj":"Disease"},{"id":"T8","span":{"begin":1271,"end":1282},"obj":"Disease"},{"id":"T9","span":{"begin":1297,"end":1305},"obj":"Disease"},{"id":"T10","span":{"begin":1356,"end":1364},"obj":"Disease"},{"id":"T11","span":{"begin":1469,"end":1480},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0017775"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0017775"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0017775"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0017775"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MONDO_0017775"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MONDO_0017775"},{"id":"A8","pred":"mondo_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/MONDO_0017775"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MONDO_0005015"},{"id":"A10","pred":"mondo_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/MONDO_0005015"},{"id":"A11","pred":"mondo_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/MONDO_0017775"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"},{"subj":"T3","pred":"source","obj":"mondo_disease"},{"subj":"T4","pred":"source","obj":"mondo_disease"},{"subj":"T5","pred":"source","obj":"mondo_disease"},{"subj":"T6","pred":"source","obj":"mondo_disease"},{"subj":"T7","pred":"source","obj":"mondo_disease"},{"subj":"T8","pred":"source","obj":"mondo_disease"},{"subj":"T9","pred":"source","obj":"mondo_disease"},{"subj":"T10","pred":"source","obj":"mondo_disease"},{"subj":"T11","pred":"source","obj":"mondo_disease"}]},{"project":"sonoma2","denotations":[{"id":"T0","span":{"begin":9,"end":16},"obj":"MPA"},{"id":"T1","span":{"begin":35,"end":45},"obj":"CPA"},{"id":"T2","span":{"begin":49,"end":81},"obj":"GENE"},{"id":"T3","span":{"begin":86,"end":102},"obj":"GENE"},{"id":"T4","span":{"begin":107,"end":118},"obj":"REG"},{"id":"T5","span":{"begin":152,"end":163},"obj":"DISEASE"},{"id":"T6","span":{"begin":174,"end":185},"obj":"DISEASE"},{"id":"T7","span":{"begin":200,"end":218},"obj":"DISEASE"},{"id":"T8","span":{"begin":219,"end":225},"obj":"REG"},{"id":"T9","span":{"begin":260,"end":267},"obj":"REG"},{"id":"T10","span":{"begin":268,"end":270},"obj":"REG"},{"id":"T11","span":{"begin":271,"end":275},"obj":"POSREG"},{"id":"T12","span":{"begin":276,"end":285},"obj":"CPA"},{"id":"T13","span":{"begin":287,"end":319},"obj":"GENE"},{"id":"T14","span":{"begin":321,"end":325},"obj":"GENE"},{"id":"T15","span":{"begin":331,"end":347},"obj":"GENE"},{"id":"T16","span":{"begin":349,"end":352},"obj":"GENE"},{"id":"T17","span":{"begin":391,"end":410},"obj":"MPA"},{"id":"T18","span":{"begin":416,"end":427},"obj":"REG"},{"id":"T19","span":{"begin":453,"end":463},"obj":"CPA"},{"id":"T20","span":{"begin":520,"end":531},"obj":"DISEASE"},{"id":"T21","span":{"begin":553,"end":563},"obj":"CPA"},{"id":"T22","span":{"begin":587,"end":598},"obj":"DISEASE"},{"id":"T23","span":{"begin":692,"end":703},"obj":"DISEASE"},{"id":"T24","span":{"begin":714,"end":718},"obj":"GENE"},{"id":"T25","span":{"begin":719,"end":726},"obj":"REG"},{"id":"T26","span":{"begin":727,"end":731},"obj":"POSREG"},{"id":"T27","span":{"begin":732,"end":735},"obj":"GENE"},{"id":"T28","span":{"begin":736,"end":742},"obj":"MPA"},{"id":"T29","span":{"begin":744,"end":747},"obj":"GENE"},{"id":"T30","span":{"begin":748,"end":755},"obj":"REG"},{"id":"T31","span":{"begin":756,"end":760},"obj":"POSREG"},{"id":"T32","span":{"begin":761,"end":764},"obj":"GENE"},{"id":"T33","span":{"begin":769,"end":772},"obj":"GENE"},{"id":"T34","span":{"begin":773,"end":779},"obj":"MPA"},{"id":"T35","span":{"begin":848,"end":860},"obj":"REG"},{"id":"T36","span":{"begin":861,"end":872},"obj":"DISEASE"},{"id":"T37","span":{"begin":903,"end":910},"obj":"POSREG"},{"id":"T38","span":{"begin":925,"end":928},"obj":"MPA"},{"id":"T39","span":{"begin":948,"end":961},"obj":"MPA"},{"id":"T40","span":{"begin":994,"end":1002},"obj":"MPA"},{"id":"T41","span":{"begin":1007,"end":1010},"obj":"GENE"},{"id":"T42","span":{"begin":1022,"end":1025},"obj":"GENE"},{"id":"T43","span":{"begin":1038,"end":1048},"obj":"REG"},{"id":"T44","span":{"begin":1060,"end":1064},"obj":"GENE"},{"id":"T45","span":{"begin":1069,"end":1077},"obj":"MPA"},{"id":"T46","span":{"begin":1078,"end":1082},"obj":"GENE"},{"id":"T47","span":{"begin":1091,"end":1099},"obj":"POSREG"},{"id":"T48","span":{"begin":1156,"end":1159},"obj":"GENE"},{"id":"T49","span":{"begin":1217,"end":1223},"obj":"POSREG"},{"id":"T50","span":{"begin":1224,"end":1245},"obj":"MPA"},{"id":"T51","span":{"begin":1234,"end":1238},"obj":"GENE"},{"id":"T52","span":{"begin":1266,"end":1282},"obj":"DISEASE"},{"id":"T53","span":{"begin":1271,"end":1282},"obj":"DISEASE"},{"id":"T54","span":{"begin":1292,"end":1296},"obj":"REG"},{"id":"T55","span":{"begin":1297,"end":1305},"obj":"DISEASE"},{"id":"T56","span":{"begin":1310,"end":1316},"obj":"POSREG"},{"id":"T57","span":{"begin":1317,"end":1336},"obj":"MPA"},{"id":"T58","span":{"begin":1348,"end":1355},"obj":"NEGREG"},{"id":"T59","span":{"begin":1356,"end":1364},"obj":"DISEASE"},{"id":"T60","span":{"begin":1393,"end":1411},"obj":"CPA"},{"id":"T61","span":{"begin":1416,"end":1426},"obj":"REG"},{"id":"T62","span":{"begin":1432,"end":1440},"obj":"REG"},{"id":"T63","span":{"begin":1441,"end":1465},"obj":"CPA"},{"id":"T64","span":{"begin":1469,"end":1480},"obj":"DISEASE"}],"relations":[{"id":"R5","pred":"ThemeOf","subj":"T2","obj":"T4"},{"id":"R6","pred":"ThemeOf","subj":"T3","obj":"T0"},{"id":"R7","pred":"ThemeOf","subj":"T3","obj":"T1"},{"id":"R8","pred":"ThemeOf","subj":"T3","obj":"T4"},{"id":"R9","pred":"ThemeOf","subj":"T5","obj":"T0"},{"id":"R10","pred":"ThemeOf","subj":"T5","obj":"T4"},{"id":"R11","pred":"ThemeOf","subj":"T6","obj":"T8"},{"id":"R12","pred":"ThemeOf","subj":"T6","obj":"T9"},{"id":"R13","pred":"ThemeOf","subj":"T6","obj":"T11"},{"id":"R53","pred":"ThemeOf","subj":"T42","obj":"T43"},{"id":"R54","pred":"ThemeOf","subj":"T44","obj":"T47"},{"id":"R55","pred":"ThemeOf","subj":"T45","obj":"T47"},{"id":"R56","pred":"ThemeOf","subj":"T46","obj":"T45"},{"id":"R57","pred":"ThemeOf","subj":"T46","obj":"T47"},{"id":"R58","pred":"ThemeOf","subj":"T48","obj":"T47"},{"id":"R59","pred":"ThemeOf","subj":"T50","obj":"T49"},{"id":"R60","pred":"ThemeOf","subj":"T53","obj":"T54"},{"id":"R61","pred":"ThemeOf","subj":"T53","obj":"T56"},{"id":"R62","pred":"ThemeOf","subj":"T55","obj":"T54"},{"id":"R63","pred":"ThemeOf","subj":"T55","obj":"T56"},{"id":"R64","pred":"ThemeOf","subj":"T57","obj":"T56"},{"id":"R65","pred":"ThemeOf","subj":"T59","obj":"T56"},{"id":"R66","pred":"ThemeOf","subj":"T59","obj":"T58"},{"id":"R67","pred":"ThemeOf","subj":"T60","obj":"T61"},{"id":"R68","pred":"ThemeOf","subj":"T60","obj":"T62"},{"id":"R69","pred":"ThemeOf","subj":"T62","obj":"T61"},{"id":"R70","pred":"ThemeOf","subj":"T63","obj":"T62"},{"id":"R71","pred":"ThemeOf","subj":"T63","obj":"T61"},{"id":"R72","pred":"ThemeOf","subj":"T64","obj":"T60"},{"id":"R73","pred":"ThemeOf","subj":"T64","obj":"T61"},{"id":"R74","pred":"ThemeOf","subj":"T64","obj":"T62"},{"id":"R75","pred":"ThemeOf","subj":"T64","obj":"T63"},{"id":"R0","pred":"ThemeOf","subj":"T1","obj":"T0"},{"id":"R1","pred":"ThemeOf","subj":"T0","obj":"T4"},{"id":"R2","pred":"ThemeOf","subj":"T1","obj":"T4"},{"id":"R3","pred":"ThemeOf","subj":"T2","obj":"T0"},{"id":"R4","pred":"ThemeOf","subj":"T2","obj":"T1"},{"id":"R14","pred":"ThemeOf","subj":"T12","obj":"T9"},{"id":"R15","pred":"ThemeOf","subj":"T12","obj":"T10"},{"id":"R16","pred":"ThemeOf","subj":"T12","obj":"T11"},{"id":"R17","pred":"ThemeOf","subj":"T13","obj":"T17"},{"id":"R18","pred":"ThemeOf","subj":"T14","obj":"T17"},{"id":"R19","pred":"ThemeOf","subj":"T15","obj":"T11"},{"id":"R20","pred":"ThemeOf","subj":"T15","obj":"T17"},{"id":"R21","pred":"ThemeOf","subj":"T16","obj":"T17"},{"id":"R22","pred":"ThemeOf","subj":"T19","obj":"T18"},{"id":"R23","pred":"ThemeOf","subj":"T20","obj":"T18"},{"id":"R24","pred":"ThemeOf","subj":"T20","obj":"T19"},{"id":"R25","pred":"ThemeOf","subj":"T22","obj":"T21"},{"id":"R26","pred":"ThemeOf","subj":"T23","obj":"T25"},{"id":"R27","pred":"ThemeOf","subj":"T23","obj":"T26"},{"id":"R28","pred":"ThemeOf","subj":"T23","obj":"T28"},{"id":"R29","pred":"CauseOf","subj":"T24","obj":"T25"},{"id":"R30","pred":"CauseOf","subj":"T24","obj":"T26"},{"id":"R31","pred":"ThemeOf","subj":"T28","obj":"T26"},{"id":"R32","pred":"ThemeOf","subj":"T27","obj":"T25"},{"id":"R33","pred":"ThemeOf","subj":"T27","obj":"T26"},{"id":"R34","pred":"ThemeOf","subj":"T27","obj":"T28"},{"id":"R35","pred":"ThemeOf","subj":"T28","obj":"T25"},{"id":"R36","pred":"CauseOf","subj":"T29","obj":"T31"},{"id":"R37","pred":"ThemeOf","subj":"T34","obj":"T31"},{"id":"R38","pred":"ThemeOf","subj":"T32","obj":"T30"},{"id":"R39","pred":"ThemeOf","subj":"T32","obj":"T31"},{"id":"R40","pred":"ThemeOf","subj":"T32","obj":"T34"},{"id":"R41","pred":"ThemeOf","subj":"T33","obj":"T30"},{"id":"R42","pred":"ThemeOf","subj":"T33","obj":"T31"},{"id":"R43","pred":"ThemeOf","subj":"T33","obj":"T34"},{"id":"R44","pred":"ThemeOf","subj":"T34","obj":"T30"},{"id":"R45","pred":"ThemeOf","subj":"T35","obj":"T37"},{"id":"R46","pred":"ThemeOf","subj":"T36","obj":"T35"},{"id":"R47","pred":"ThemeOf","subj":"T36","obj":"T37"},{"id":"R48","pred":"ThemeOf","subj":"T38","obj":"T37"},{"id":"R49","pred":"ThemeOf","subj":"T39","obj":"T37"},{"id":"R50","pred":"ThemeOf","subj":"T41","obj":"T40"},{"id":"R51","pred":"ThemeOf","subj":"T40","obj":"T43"},{"id":"R52","pred":"ThemeOf","subj":"T41","obj":"T43"}],"attributes":[{"subj":"T0","pred":"source","obj":"sonoma2"},{"subj":"T1","pred":"source","obj":"sonoma2"},{"subj":"T2","pred":"source","obj":"sonoma2"},{"subj":"T3","pred":"source","obj":"sonoma2"},{"subj":"T4","pred":"source","obj":"sonoma2"},{"subj":"T5","pred":"source","obj":"sonoma2"},{"subj":"T6","pred":"source","obj":"sonoma2"},{"subj":"T7","pred":"source","obj":"sonoma2"},{"subj":"T8","pred":"source","obj":"sonoma2"},{"subj":"T9","pred":"source","obj":"sonoma2"},{"subj":"T10","pred":"source","obj":"sonoma2"},{"subj":"T11","pred":"source","obj":"sonoma2"},{"subj":"T12","pred":"source","obj":"sonoma2"},{"subj":"T13","pred":"source","obj":"sonoma2"},{"subj":"T14","pred":"source","obj":"sonoma2"},{"subj":"T15","pred":"source","obj":"sonoma2"},{"subj":"T16","pred":"source","obj":"sonoma2"},{"subj":"T17","pred":"source","obj":"sonoma2"},{"subj":"T18","pred":"source","obj":"sonoma2"},{"subj":"T19","pred":"source","obj":"sonoma2"},{"subj":"T20","pred":"source","obj":"sonoma2"},{"subj":"T21","pred":"source","obj":"sonoma2"},{"subj":"T22","pred":"source","obj":"sonoma2"},{"subj":"T23","pred":"source","obj":"sonoma2"},{"subj":"T24","pred":"source","obj":"sonoma2"},{"subj":"T25","pred":"source","obj":"sonoma2"},{"subj":"T26","pred":"source","obj":"sonoma2"},{"subj":"T27","pred":"source","obj":"sonoma2"},{"subj":"T28","pred":"source","obj":"sonoma2"},{"subj":"T29","pred":"source","obj":"sonoma2"},{"subj":"T30","pred":"source","obj":"sonoma2"},{"subj":"T31","pred":"source","obj":"sonoma2"},{"subj":"T32","pred":"source","obj":"sonoma2"},{"subj":"T33","pred":"source","obj":"sonoma2"},{"subj":"T34","pred":"source","obj":"sonoma2"},{"subj":"T35","pred":"source","obj":"sonoma2"},{"subj":"T36","pred":"source","obj":"sonoma2"},{"subj":"T37","pred":"source","obj":"sonoma2"},{"subj":"T38","pred":"source","obj":"sonoma2"},{"subj":"T39","pred":"source","obj":"sonoma2"},{"subj":"T40","pred":"source","obj":"sonoma2"},{"subj":"T41","pred":"source","obj":"sonoma2"},{"subj":"T42","pred":"source","obj":"sonoma2"},{"subj":"T43","pred":"source","obj":"sonoma2"},{"subj":"T44","pred":"source","obj":"sonoma2"},{"subj":"T45","pred":"source","obj":"sonoma2"},{"subj":"T46","pred":"source","obj":"sonoma2"},{"subj":"T47","pred":"source","obj":"sonoma2"},{"subj":"T48","pred":"source","obj":"sonoma2"},{"subj":"T49","pred":"source","obj":"sonoma2"},{"subj":"T50","pred":"source","obj":"sonoma2"},{"subj":"T51","pred":"source","obj":"sonoma2"},{"subj":"T52","pred":"source","obj":"sonoma2"},{"subj":"T53","pred":"source","obj":"sonoma2"},{"subj":"T54","pred":"source","obj":"sonoma2"},{"subj":"T55","pred":"source","obj":"sonoma2"},{"subj":"T56","pred":"source","obj":"sonoma2"},{"subj":"T57","pred":"source","obj":"sonoma2"},{"subj":"T58","pred":"source","obj":"sonoma2"},{"subj":"T59","pred":"source","obj":"sonoma2"},{"subj":"T60","pred":"source","obj":"sonoma2"},{"subj":"T61","pred":"source","obj":"sonoma2"},{"subj":"T62","pred":"source","obj":"sonoma2"},{"subj":"T63","pred":"source","obj":"sonoma2"},{"subj":"T64","pred":"source","obj":"sonoma2"}]},{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":1266,"end":1270},"obj":"Body_part"},{"id":"T2","span":{"begin":1342,"end":1347},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000029"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000029"},{"subj":"T1","pred":"source","obj":"Anatomy-MAT"},{"subj":"T2","pred":"source","obj":"Anatomy-MAT"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":567,"end":573},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0001969"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"NCBITAXON","color":"#ecab93","default":true},{"id":"mondo_disease","color":"#9394ec"},{"id":"sonoma2","color":"#aeec93"},{"id":"Anatomy-MAT","color":"#ec93c8"},{"id":"Anatomy-UBERON","color":"#93e3ec"}]}]}}