PubMed:3155737 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/3155737","sourcedb":"PubMed","sourceid":"3155737","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/3155737","text":"Phosphorylation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase and modulation of its enzymic activity by calcium-activated and phospholipid-dependent protein kinase.\nA calcium-activated and phospholipid-dependent protein kinase (protein kinase C) catalyzes the phosphorylation of both insoluble microsomal (Mr approximately 100,000) and purified soluble (Mr = 53,000) 3-hydroxy-3-methylglutaryl coenzyme A reductase. The phosphorylation and concomitant inactivation of enzymic activity of HMG-CoA reductase was absolutely dependent on Ca2+, phosphatidylserine, and diolein. Dephosphorylation of phosphorylated HMG-CoA reductase was associated with the loss of protein bound radioactivity and reactivation of enzymic activity. Maximal phosphorylation of purified HMG-CoA reductase was associated with the incorporation of 1.05 +/- 0.016 mol of phosphate/mol of native form of HMG-CoA reductase (Mr approximately 100,000). The apparent Km for purified HMG-CoA reductase and histone H1 was 0.08 mg/ml, and 0.12 mg/ml, respectively. The tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate stimulated the protein kinase C-catalyzed phosphorylation of HMG-CoA reductase. Increased phosphorylation of HMG-CoA reductase by phorbol 12-myristate 13-acetate suggests a possible in vivo protein kinase C-mediated mechanism for the short-term regulation of HMG-CoA reductase activity. The identification of the protein kinase C system in addition to the reductase kinase-reductase kinase kinase bicyclic cascade systems for the modulation of the enzymic activity of HMG-CoA reductase may provide new insights into the molecular mechanisms involved in the regulation of cholesterol biosynthesis.","tracks":[{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":177},"obj":"Sentence"},{"id":"T2","span":{"begin":178,"end":428},"obj":"Sentence"},{"id":"T3","span":{"begin":429,"end":585},"obj":"Sentence"},{"id":"T4","span":{"begin":586,"end":737},"obj":"Sentence"},{"id":"T5","span":{"begin":738,"end":932},"obj":"Sentence"},{"id":"T6","span":{"begin":933,"end":1040},"obj":"Sentence"},{"id":"T7","span":{"begin":1041,"end":1187},"obj":"Sentence"},{"id":"T8","span":{"begin":1188,"end":1394},"obj":"Sentence"},{"id":"T9","span":{"begin":1395,"end":1704},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":177},"obj":"Sentence"},{"id":"T2","span":{"begin":178,"end":428},"obj":"Sentence"},{"id":"T3","span":{"begin":429,"end":585},"obj":"Sentence"},{"id":"T4","span":{"begin":586,"end":737},"obj":"Sentence"},{"id":"T5","span":{"begin":738,"end":932},"obj":"Sentence"},{"id":"T6","span":{"begin":933,"end":1040},"obj":"Sentence"},{"id":"T7","span":{"begin":1041,"end":1187},"obj":"Sentence"},{"id":"T8","span":{"begin":1188,"end":1394},"obj":"Sentence"},{"id":"T9","span":{"begin":1395,"end":1704},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":1045,"end":1050},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005070"},{"subj":"T1","pred":"source","obj":"mondo_disease"}]},{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":1045,"end":1050},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002664"},{"subj":"T1","pred":"source","obj":"HP-phenotype"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#93d4ec","default":true},{"id":"mondo_disease","color":"#ece993"},{"id":"HP-phenotype","color":"#cf93ec"}]}]}}