> top > docs > PubMed:31416836 > annotations

PubMed:31416836 JSONTXT

Annnotations TAB JSON ListView MergeView

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 415-424 Glycan_Motif denotes galactose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE

sentences

Id Subject Object Predicate Lexical cue
T1 0-120 Sentence denotes Ega3 from the fungal pathogen Aspergillus fumigatus is an endo-α-1,4-galactosaminidase that disrupts microbial biofilms.
T2 121-234 Sentence denotes Aspergillus fumigatus is an opportunistic fungal pathogen that causes both chronic and acute invasive infections.
T3 235-350 Sentence denotes Galactosaminogalactan (GAG) is an integral component of the A. fumigatus biofilm matrix and a key virulence factor.
T4 351-483 Sentence denotes GAG is a heterogeneous linear α-1,4-linked exopolysaccharide of galactose and GalNAc that is partially deacetylated after secretion.
T5 484-574 Sentence denotes A cluster of five co-expressed genes has been linked to GAG biosynthesis and modification.
T6 575-719 Sentence denotes One gene in this cluster, ega3, is annotated as encoding a putative α-1,4-galactosaminidase belonging to glycoside hydrolase family 114 (GH114).
T7 720-925 Sentence denotes Herein, we show that recombinant Ega3 is an active glycoside hydrolase that disrupts GAG-dependent A. fumigatus and Pel polysaccharide-dependent Pseudomonas aeruginosa biofilms at nanomolar concentrations.
T8 926-1102 Sentence denotes Using MS and functional assays, we demonstrate that Ega3 is an endo-acting α-1,4-galactosaminidase whose activity depends on the conserved acidic residues, Asp-189 and Glu-247.
T9 1103-1349 Sentence denotes X-ray crystallographic structural analysis of the apo Ega3 and an Ega3-galactosamine complex, at 1.76 and 2.09 Å resolutions, revealed a modified (β/α)8-fold with a deep electronegative cleft, which upon ligand binding is capped to form a tunnel.
T10 1350-1540 Sentence denotes Our structural analysis coupled with in silico docking studies also uncovered the molecular determinants for galactosamine specificity and substrate binding at the -2 to +1 binding subsites.
T11 1541-1769 Sentence denotes The findings in this study increase the structural and mechanistic understanding of the GH114 family, which has >600 members encoded by plant and opportunistic human pathogens, as well as in industrially used bacteria and fungi.
T1 0-120 Sentence denotes Ega3 from the fungal pathogen Aspergillus fumigatus is an endo-α-1,4-galactosaminidase that disrupts microbial biofilms.
T2 121-234 Sentence denotes Aspergillus fumigatus is an opportunistic fungal pathogen that causes both chronic and acute invasive infections.
T3 235-350 Sentence denotes Galactosaminogalactan (GAG) is an integral component of the A. fumigatus biofilm matrix and a key virulence factor.
T4 351-483 Sentence denotes GAG is a heterogeneous linear α-1,4-linked exopolysaccharide of galactose and GalNAc that is partially deacetylated after secretion.
T5 484-574 Sentence denotes A cluster of five co-expressed genes has been linked to GAG biosynthesis and modification.
T6 575-719 Sentence denotes One gene in this cluster, ega3, is annotated as encoding a putative α-1,4-galactosaminidase belonging to glycoside hydrolase family 114 (GH114).
T7 720-925 Sentence denotes Herein, we show that recombinant Ega3 is an active glycoside hydrolase that disrupts GAG-dependent A. fumigatus and Pel polysaccharide-dependent Pseudomonas aeruginosa biofilms at nanomolar concentrations.
T8 926-1102 Sentence denotes Using MS and functional assays, we demonstrate that Ega3 is an endo-acting α-1,4-galactosaminidase whose activity depends on the conserved acidic residues, Asp-189 and Glu-247.
T9 1103-1349 Sentence denotes X-ray crystallographic structural analysis of the apo Ega3 and an Ega3-galactosamine complex, at 1.76 and 2.09 Å resolutions, revealed a modified (β/α)8-fold with a deep electronegative cleft, which upon ligand binding is capped to form a tunnel.
T10 1350-1540 Sentence denotes Our structural analysis coupled with in silico docking studies also uncovered the molecular determinants for galactosamine specificity and substrate binding at the -2 to +1 binding subsites.
T11 1541-1769 Sentence denotes The findings in this study increase the structural and mechanistic understanding of the GH114 family, which has >600 members encoded by plant and opportunistic human pathogens, as well as in industrially used bacteria and fungi.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 415-424 https://glytoucan.org/Structures/Glycans/G65889KE denotes galactose
T2 415-424 https://glytoucan.org/Structures/Glycans/G68158BT denotes galactose

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 429-435 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 223-233 Disease denotes infections http://purl.obolibrary.org/obo/MONDO_0005550

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 429-435 Glycan denotes GalNAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 223-233 Disease denotes infections MONDO:0005550

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 30-51 Organism denotes Aspergillus fumigatus 746128
T2 121-142 Organism denotes Aspergillus fumigatus 746128
T3 865-887 Organism denotes Pseudomonas aeruginosa 287
T4 1701-1706 Organism denotes human 9606
T5 1750-1758 Organism denotes bacteria 2|629395
T7 1763-1768 Organism denotes fungi 4751

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-120 Sentence denotes Ega3 from the fungal pathogen Aspergillus fumigatus is an endo-α-1,4-galactosaminidase that disrupts microbial biofilms.
T2 121-234 Sentence denotes Aspergillus fumigatus is an opportunistic fungal pathogen that causes both chronic and acute invasive infections.
T3 235-350 Sentence denotes Galactosaminogalactan (GAG) is an integral component of the A. fumigatus biofilm matrix and a key virulence factor.
T4 351-483 Sentence denotes GAG is a heterogeneous linear α-1,4-linked exopolysaccharide of galactose and GalNAc that is partially deacetylated after secretion.
T5 484-574 Sentence denotes A cluster of five co-expressed genes has been linked to GAG biosynthesis and modification.
T6 575-719 Sentence denotes One gene in this cluster, ega3, is annotated as encoding a putative α-1,4-galactosaminidase belonging to glycoside hydrolase family 114 (GH114).
T7 720-925 Sentence denotes Herein, we show that recombinant Ega3 is an active glycoside hydrolase that disrupts GAG-dependent A. fumigatus and Pel polysaccharide-dependent Pseudomonas aeruginosa biofilms at nanomolar concentrations.
T8 926-1102 Sentence denotes Using MS and functional assays, we demonstrate that Ega3 is an endo-acting α-1,4-galactosaminidase whose activity depends on the conserved acidic residues, Asp-189 and Glu-247.
T9 1103-1349 Sentence denotes X-ray crystallographic structural analysis of the apo Ega3 and an Ega3-galactosamine complex, at 1.76 and 2.09 Å resolutions, revealed a modified (β/α)8-fold with a deep electronegative cleft, which upon ligand binding is capped to form a tunnel.
T10 1350-1540 Sentence denotes Our structural analysis coupled with in silico docking studies also uncovered the molecular determinants for galactosamine specificity and substrate binding at the -2 to +1 binding subsites.
T11 1541-1769 Sentence denotes The findings in this study increase the structural and mechanistic understanding of the GH114 family, which has >600 members encoded by plant and opportunistic human pathogens, as well as in industrially used bacteria and fungi.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 30-51 OrganismTaxon denotes Aspergillus fumigatus 746128
T2 121-142 OrganismTaxon denotes Aspergillus fumigatus 746128
T3 865-887 OrganismTaxon denotes Pseudomonas aeruginosa 287
T4 1701-1706 OrganismTaxon denotes human 9606
T5 1750-1758 OrganismTaxon denotes bacteria 2|629395
T7 1763-1768 OrganismTaxon denotes fungi 4751