| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-77 |
Sentence |
denotes |
Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin. |
| T2 |
78-80 |
Sentence |
denotes |
I. |
| T3 |
81-212 |
Sentence |
denotes |
Structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glycoprotein hormones. |
| T4 |
213-456 |
Sentence |
denotes |
We have elucidated the structures of the anionic asparagine-linked oligosaccharides present on the glycoprotein hormones lutropin (luteinizing hormone), follitropin (follicle-stimulating hormone), and thyrotropin (thyroid-stimulating hormone). |
| T5 |
457-621 |
Sentence |
denotes |
Purified hormones, isolated from bovine, ovine, and human pituitaries, were digested with N-glycanase, and the released oligosaccharides were reduced with NaB[3H]4. |
| T6 |
622-839 |
Sentence |
denotes |
The 3H-labeled oligosaccharides from each hormone were then fractionated by anion-exchange high performance liquid chromatography (HPLC) into populations differing in the number of sulfate and/or sialic acid moieties. |
| T7 |
840-1075 |
Sentence |
denotes |
The anionic oligosaccharides were further purified as well as structurally characterized using a variety of preparative and analytical techniques, including HPLC, endo- and exoglycosidase digestions, and lectin affinity chromatography. |
| T8 |
1076-1345 |
Sentence |
denotes |
The sulfated, sialylated, and sulfated/sialylated structures, which together comprised 67-90% of the asparagine-linked oligosaccharides on the pituitary glycoprotein hormones, were highly heterogeneous and displayed hormone- as well as animal species-specific features. |
| T9 |
1346-1491 |
Sentence |
denotes |
The sulfated oligosaccharides consisted of hybrid and complex type oligosaccharides with one or two branches terminating in SO4-4GalNAc beta 1,4. |
| T10 |
1492-1682 |
Sentence |
denotes |
In contrast, the sialylated oligosaccharides consisted of a wide array of differing structures containing two or three peripheral branches as well as one, two, or three sialic acid moieties. |
| T11 |
1683-1849 |
Sentence |
denotes |
A previously uncharacterized dibranched oligosaccharide, bearing one residue each of sulfate and sialic acid, was found on all of the hormones except bovine lutropin. |
| T12 |
1850-2082 |
Sentence |
denotes |
In this study, we describe the purification and detailed structural characterizations of the sulfated, sialylated, and sulfated/sialylated oligosaccharides found on lutropin, follitropin, and thyrotropin from several animal species. |
| T13 |
2083-2151 |
Sentence |
denotes |
In the accompanying paper (Green, E.D., and Baenziger, J.U.(1987) J. |
| T14 |
2152-2157 |
Sentence |
denotes |
Biol. |
| T15 |
2158-2163 |
Sentence |
denotes |
Chem. |
| T16 |
2164-2447 |
Sentence |
denotes |
262, 36-44) we demonstrate the marked quantitative differences among the pituitary glycoprotein hormones in terms of sulfation, sialylation, and underlying oligosaccharide structures, as well as provide evidence for site-specific synthesis of oligosaccharides on individual hormones. |
| T1 |
0-77 |
Sentence |
denotes |
Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin. |
| T2 |
78-80 |
Sentence |
denotes |
I. |
| T3 |
81-212 |
Sentence |
denotes |
Structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glycoprotein hormones. |
| T4 |
213-456 |
Sentence |
denotes |
We have elucidated the structures of the anionic asparagine-linked oligosaccharides present on the glycoprotein hormones lutropin (luteinizing hormone), follitropin (follicle-stimulating hormone), and thyrotropin (thyroid-stimulating hormone). |
| T5 |
457-621 |
Sentence |
denotes |
Purified hormones, isolated from bovine, ovine, and human pituitaries, were digested with N-glycanase, and the released oligosaccharides were reduced with NaB[3H]4. |
| T6 |
622-839 |
Sentence |
denotes |
The 3H-labeled oligosaccharides from each hormone were then fractionated by anion-exchange high performance liquid chromatography (HPLC) into populations differing in the number of sulfate and/or sialic acid moieties. |
| T7 |
840-1075 |
Sentence |
denotes |
The anionic oligosaccharides were further purified as well as structurally characterized using a variety of preparative and analytical techniques, including HPLC, endo- and exoglycosidase digestions, and lectin affinity chromatography. |
| T8 |
1076-1345 |
Sentence |
denotes |
The sulfated, sialylated, and sulfated/sialylated structures, which together comprised 67-90% of the asparagine-linked oligosaccharides on the pituitary glycoprotein hormones, were highly heterogeneous and displayed hormone- as well as animal species-specific features. |
| T9 |
1346-1491 |
Sentence |
denotes |
The sulfated oligosaccharides consisted of hybrid and complex type oligosaccharides with one or two branches terminating in SO4-4GalNAc beta 1,4. |
| T10 |
1492-1682 |
Sentence |
denotes |
In contrast, the sialylated oligosaccharides consisted of a wide array of differing structures containing two or three peripheral branches as well as one, two, or three sialic acid moieties. |
| T11 |
1683-1849 |
Sentence |
denotes |
A previously uncharacterized dibranched oligosaccharide, bearing one residue each of sulfate and sialic acid, was found on all of the hormones except bovine lutropin. |
| T12 |
1850-2082 |
Sentence |
denotes |
In this study, we describe the purification and detailed structural characterizations of the sulfated, sialylated, and sulfated/sialylated oligosaccharides found on lutropin, follitropin, and thyrotropin from several animal species. |
| T13 |
2083-2151 |
Sentence |
denotes |
In the accompanying paper (Green, E.D., and Baenziger, J.U.(1987) J. |
| T14 |
2152-2157 |
Sentence |
denotes |
Biol. |
| T15 |
2158-2163 |
Sentence |
denotes |
Chem. |
| T16 |
2164-2447 |
Sentence |
denotes |
262, 36-44) we demonstrate the marked quantitative differences among the pituitary glycoprotein hormones in terms of sulfation, sialylation, and underlying oligosaccharide structures, as well as provide evidence for site-specific synthesis of oligosaccharides on individual hormones. |