| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
114-252 |
DRI_Outcome |
denotes |
In this study the evolution in the efficiency of electrochemotherapy (reversible electroporation) with pulse number was assessed in vitro. |
| T2 |
253-381 |
DRI_Approach |
denotes |
Experiments were performed using 100 μs pulses at different electric field intensities and the chemotherapeutic agent bleomycin. |
| T3 |
382-581 |
DRI_Background |
denotes |
Additionally, electrical impedance spectroscopy measurements were used as a different method to study in real time the changes produced on cells with pulse number during trains of consecutive pulses. |
| T4 |
582-710 |
DRI_Outcome |
denotes |
Our results show that the relation between pulse number and the observed outcome is complex and difficult to fully characterize. |
| T5 |
711-827 |
DRI_Background |
denotes |
This relation can display a highly linear behaviour up to a certain number of pulses and/or field intensity applied. |
| T6 |
828-1074 |
DRI_Outcome |
denotes |
However, the relation between the number of pulses and the observed outcome always evolves to a saturation or at least a reduction in the electric field effects that is displayed when either electric field intensity or pulse number are increased. |
| T7 |
1075-1192 |
DRI_Approach |
denotes |
An exponential model was found to best describe this relation within the range of experimental conditions considered. |
| T8 |
1193-1307 |
DRI_Background |
denotes |
Electrical impedance measurements confirmed the results and gave a more precise quantification of this dependence. |
| T9 |
1308-1460 |
DRI_Approach |
denotes |
The study highlights the importance that pulse number has in the electrochemotherapy protocols and establishes some limits in the use of this parameter. |
