> top > docs > PubMed:31055697 > annotations

PubMed:31055697 JSONTXT

Annnotations TAB JSON ListView MergeView

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 687-694 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T3 699-720 Glycan_Motif denotes chondroitin-6-sulfate https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G34992DF
T4 699-710 Glycan_Motif denotes chondroitin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT
T5 1207-1214 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T7 1275-1282 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 699-720 http://www.glycoepitope.jp/epitopes/EP0083 denotes chondroitin-6-sulfate
T2 699-710 http://www.glycoepitope.jp/epitopes/EP0081 denotes chondroitin

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 687-694 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T2 687-694 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T3 699-720 https://glytoucan.org/Structures/Glycans/G34992DF denotes chondroitin-6-sulfate
T4 699-710 https://glytoucan.org/Structures/Glycans/G43702JT denotes chondroitin
T5 1207-1214 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T6 1207-1214 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T7 1275-1282 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T8 1275-1282 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-98 Sentence denotes A versatile salt-based method to immobilize glycosaminoglycans and create growth factor gradients.
TextSentencer_T2 99-212 Sentence denotes Glycosaminoglycans (GAGs) are known to play pivotal roles in physiological processes and pathological conditions.
TextSentencer_T3 213-299 Sentence denotes To study interactions of GAGs with proteins, immobilization of GAGs is often required.
TextSentencer_T4 300-453 Sentence denotes Current methodologies for immobilization involve modification of GAGs and/or surfaces, which can be time-consuming and may involve specialized equipment.
TextSentencer_T5 454-596 Sentence denotes Here, we use an efficient and low-cost method to immobilize GAGs without any (chemical) modification using highly concentrated salt solutions.
TextSentencer_T6 597-810 Sentence denotes A number of salts from the Hofmeister series were probed for their capacity to immobilize heparin and chondroitin-6-sulfate on microtiter plates applying single chain antibodies against GAGs for detection (ELISA).
TextSentencer_T7 811-953 Sentence denotes From all salts tested, the cosmotropic salt ammonium sulfate was most efficient, especially at high concentrations (80-100% (v/v) saturation).
TextSentencer_T8 954-1128 Sentence denotes Immobilized GAGs were bioavailable as judged by their binding of FGF2 and VEGF, and by their susceptibility towards GAG lyases (heparinase I, II and III, chondroitinase ABC).
TextSentencer_T9 1129-1312 Sentence denotes Using 80% (v/v) saturated ammonium sulfate, block and continuous gradients of heparin were established and a gradient of FGF2 was created using a heparin block gradient as a template.
TextSentencer_T10 1313-1544 Sentence denotes In conclusion, high concentrations of ammonium sulfate are effective for immobilization of GAGs and for the establishment of gradients of both GAGs and GAG-binding molecules, which enables the study to the biological roles of GAGs.
T1 0-98 Sentence denotes A versatile salt-based method to immobilize glycosaminoglycans and create growth factor gradients.
T2 99-212 Sentence denotes Glycosaminoglycans (GAGs) are known to play pivotal roles in physiological processes and pathological conditions.
T3 213-299 Sentence denotes To study interactions of GAGs with proteins, immobilization of GAGs is often required.
T4 300-453 Sentence denotes Current methodologies for immobilization involve modification of GAGs and/or surfaces, which can be time-consuming and may involve specialized equipment.
T5 454-596 Sentence denotes Here, we use an efficient and low-cost method to immobilize GAGs without any (chemical) modification using highly concentrated salt solutions.
T6 597-810 Sentence denotes A number of salts from the Hofmeister series were probed for their capacity to immobilize heparin and chondroitin-6-sulfate on microtiter plates applying single chain antibodies against GAGs for detection (ELISA).
T7 811-953 Sentence denotes From all salts tested, the cosmotropic salt ammonium sulfate was most efficient, especially at high concentrations (80-100% (v/v) saturation).
T8 954-1128 Sentence denotes Immobilized GAGs were bioavailable as judged by their binding of FGF2 and VEGF, and by their susceptibility towards GAG lyases (heparinase I, II and III, chondroitinase ABC).
T9 1129-1312 Sentence denotes Using 80% (v/v) saturated ammonium sulfate, block and continuous gradients of heparin were established and a gradient of FGF2 was created using a heparin block gradient as a template.
T10 1313-1544 Sentence denotes In conclusion, high concentrations of ammonium sulfate are effective for immobilization of GAGs and for the establishment of gradients of both GAGs and GAG-binding molecules, which enables the study to the biological roles of GAGs.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 699-720 Glycan denotes chondroitin-6-sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G34992DF

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 699-720 Glycan denotes chondroitin-6-sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G34992DF

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 699-720 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin-6-sulfate http://www.glycoepitope.jp/epitopes/EP0083

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-98 Sentence denotes A versatile salt-based method to immobilize glycosaminoglycans and create growth factor gradients.
T2 99-212 Sentence denotes Glycosaminoglycans (GAGs) are known to play pivotal roles in physiological processes and pathological conditions.
T3 213-299 Sentence denotes To study interactions of GAGs with proteins, immobilization of GAGs is often required.
T4 300-453 Sentence denotes Current methodologies for immobilization involve modification of GAGs and/or surfaces, which can be time-consuming and may involve specialized equipment.
T5 454-596 Sentence denotes Here, we use an efficient and low-cost method to immobilize GAGs without any (chemical) modification using highly concentrated salt solutions.
T6 597-810 Sentence denotes A number of salts from the Hofmeister series were probed for their capacity to immobilize heparin and chondroitin-6-sulfate on microtiter plates applying single chain antibodies against GAGs for detection (ELISA).
T7 811-953 Sentence denotes From all salts tested, the cosmotropic salt ammonium sulfate was most efficient, especially at high concentrations (80-100% (v/v) saturation).
T8 954-1128 Sentence denotes Immobilized GAGs were bioavailable as judged by their binding of FGF2 and VEGF, and by their susceptibility towards GAG lyases (heparinase I, II and III, chondroitinase ABC).
T9 1129-1312 Sentence denotes Using 80% (v/v) saturated ammonium sulfate, block and continuous gradients of heparin were established and a gradient of FGF2 was created using a heparin block gradient as a template.
T10 1313-1544 Sentence denotes In conclusion, high concentrations of ammonium sulfate are effective for immobilization of GAGs and for the establishment of gradients of both GAGs and GAG-binding molecules, which enables the study to the biological roles of GAGs.

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 699-720 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin-6-sulfate http://www.glycoepitope.jp/epitopes/EP0083