| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-135 |
Sentence |
denotes |
Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis. |
| TextSentencer_T2 |
136-231 |
Sentence |
denotes |
Lactobacillus farciminis CIP 103136 is a bacterial strain with recognized probiotic properties. |
| TextSentencer_T3 |
232-319 |
Sentence |
denotes |
However, the mechanisms underlying such properties have only been partially elucidated. |
| TextSentencer_T4 |
320-449 |
Sentence |
denotes |
In this study, we isolated and purified a cell-wall associated polysaccharide (CWPS), and evaluated its biological role in vitro. |
| TextSentencer_T5 |
450-747 |
Sentence |
denotes |
The structure of CWPS and responses from stimulation of (i) human macrophage-like THP-1 cells, (ii) human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR2 or TLR4) and (iii) human colonocyte-like T84 intestinal epithelial cells, upon exposure to CWPS were studied. |
| TextSentencer_T6 |
748-957 |
Sentence |
denotes |
The structure of the purified CWPS from L. farciminis CIP 103136 was analyzed by nuclear magnetic resonance (NMR), MALDI-TOF-TOF MS, and methylation analyses in its native form and following Smith degradation. |
| TextSentencer_T7 |
958-1185 |
Sentence |
denotes |
It was shown to be a novel branched polysaccharide, composed of linear backbone of trisaccharide repeating units of: [→6αGlcpNAc1 → 4βManpNAc1 → 4βGlcpNAc1→] highly substituted with single residues of αGlcp, αGalp and αGlcpNAc. |
| TextSentencer_T8 |
1186-1304 |
Sentence |
denotes |
Subsequently, the lack of pro- or anti-inflammatory properties of CWPS was established on macrophage-like THP-1 cells. |
| TextSentencer_T9 |
1305-1418 |
Sentence |
denotes |
In addition, CWPS failed to modulate cell signaling pathways dependent of TLR2 and TLR4 in transfected HEK-cells. |
| TextSentencer_T10 |
1419-1584 |
Sentence |
denotes |
Finally, in T84 cells, CWPS neither influenced intestinal barrier integrity under basal conditions nor prevented TNF-α/IFN-γ cytokine-mediated epithelium impairment. |
| T1 |
0-135 |
Sentence |
denotes |
Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis. |
| T2 |
136-231 |
Sentence |
denotes |
Lactobacillus farciminis CIP 103136 is a bacterial strain with recognized probiotic properties. |
| T3 |
232-319 |
Sentence |
denotes |
However, the mechanisms underlying such properties have only been partially elucidated. |
| T4 |
320-449 |
Sentence |
denotes |
In this study, we isolated and purified a cell-wall associated polysaccharide (CWPS), and evaluated its biological role in vitro. |
| T5 |
450-747 |
Sentence |
denotes |
The structure of CWPS and responses from stimulation of (i) human macrophage-like THP-1 cells, (ii) human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR2 or TLR4) and (iii) human colonocyte-like T84 intestinal epithelial cells, upon exposure to CWPS were studied. |
| T6 |
748-957 |
Sentence |
denotes |
The structure of the purified CWPS from L. farciminis CIP 103136 was analyzed by nuclear magnetic resonance (NMR), MALDI-TOF-TOF MS, and methylation analyses in its native form and following Smith degradation. |
| T7 |
958-1185 |
Sentence |
denotes |
It was shown to be a novel branched polysaccharide, composed of linear backbone of trisaccharide repeating units of: [→6αGlcpNAc1 → 4βManpNAc1 → 4βGlcpNAc1→] highly substituted with single residues of αGlcp, αGalp and αGlcpNAc. |
| T8 |
1186-1304 |
Sentence |
denotes |
Subsequently, the lack of pro- or anti-inflammatory properties of CWPS was established on macrophage-like THP-1 cells. |
| T9 |
1305-1418 |
Sentence |
denotes |
In addition, CWPS failed to modulate cell signaling pathways dependent of TLR2 and TLR4 in transfected HEK-cells. |
| T10 |
1419-1584 |
Sentence |
denotes |
Finally, in T84 cells, CWPS neither influenced intestinal barrier integrity under basal conditions nor prevented TNF-α/IFN-γ cytokine-mediated epithelium impairment. |
