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PubMed:3036817 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-132 Sentence denotes Expression in Escherichia coli of BCY1, the regulatory subunit of cyclic AMP-dependent protein kinase from Saccharomyces cerevisiae.
T2 133-167 Sentence denotes Purification and characterization.
T3 168-419 Sentence denotes The regulatory (R) subunit of cAMP-dependent protein kinase from the yeast Saccharomyces cerevisiae was expressed in Escherichia coli by engineering the gene for yeast R, BCY1, into an E. coli expression vector that contained a promoter from phage T7.
T4 420-535 Sentence denotes Oligonucleotide-directed mutagenesis was used to create an NdeI restriction site at the natural ATG of the yeast R.
T5 536-678 Sentence denotes This facilitated construction of the T7 expression vector so that the sequence of the protein produced was identical to the natural R subunit.
T6 679-726 Sentence denotes Yeast R was highly expressed in a soluble form.
T7 727-791 Sentence denotes 20 mg of purified yeast R was obtained from 4 liters of E. coli.
T8 792-893 Sentence denotes N-terminal amino acid sequencing revealed that the expressed protein began with the natural sequence.
T9 894-1015 Sentence denotes 60% of the molecules contained an N-terminal methionine, and 40% initiated with valine, the second amino acid of yeast R.
T10 1016-1125 Sentence denotes The protein produced in E. coli migrated on a sodium dodecyl sulfate-polyacrylamide gel with an Mr of 52,000.
T11 1126-1194 Sentence denotes The yeast R bound 2 mol of cAMP/mol of R monomer with a Kd of 76 nM.
T12 1195-1250 Sentence denotes The protein was treated with urea to remove bound cAMP.
T13 1251-1337 Sentence denotes Sedimentation values before and after the urea treatment were identical (s20,w = 5.1).
T14 1338-1485 Sentence denotes Addition of purified R subunit to a preparation of yeast C subunit (TPK1) rendered catalytic activity cAMP-dependent with an activity ratio of 4.6.
T15 1486-1584 Sentence denotes The yeast R was autophosphorylated by yeast C to a level of 0.8 mol of phosphate/mol of R monomer.
T16 1585-1757 Sentence denotes By these criteria, the R subunit produced in E. coli was structurally and functionally identical to the natural yeast R subunit and similar to mammalian type II R subunits.
T1 0-132 Sentence denotes Expression in Escherichia coli of BCY1, the regulatory subunit of cyclic AMP-dependent protein kinase from Saccharomyces cerevisiae.
T2 133-167 Sentence denotes Purification and characterization.
T3 168-419 Sentence denotes The regulatory (R) subunit of cAMP-dependent protein kinase from the yeast Saccharomyces cerevisiae was expressed in Escherichia coli by engineering the gene for yeast R, BCY1, into an E. coli expression vector that contained a promoter from phage T7.
T4 420-535 Sentence denotes Oligonucleotide-directed mutagenesis was used to create an NdeI restriction site at the natural ATG of the yeast R.
T5 536-678 Sentence denotes This facilitated construction of the T7 expression vector so that the sequence of the protein produced was identical to the natural R subunit.
T6 679-726 Sentence denotes Yeast R was highly expressed in a soluble form.
T7 727-791 Sentence denotes 20 mg of purified yeast R was obtained from 4 liters of E. coli.
T8 792-893 Sentence denotes N-terminal amino acid sequencing revealed that the expressed protein began with the natural sequence.
T9 894-1015 Sentence denotes 60% of the molecules contained an N-terminal methionine, and 40% initiated with valine, the second amino acid of yeast R.
T10 1016-1125 Sentence denotes The protein produced in E. coli migrated on a sodium dodecyl sulfate-polyacrylamide gel with an Mr of 52,000.
T11 1126-1194 Sentence denotes The yeast R bound 2 mol of cAMP/mol of R monomer with a Kd of 76 nM.
T12 1195-1250 Sentence denotes The protein was treated with urea to remove bound cAMP.
T13 1251-1337 Sentence denotes Sedimentation values before and after the urea treatment were identical (s20,w = 5.1).
T14 1338-1485 Sentence denotes Addition of purified R subunit to a preparation of yeast C subunit (TPK1) rendered catalytic activity cAMP-dependent with an activity ratio of 4.6.
T15 1486-1584 Sentence denotes The yeast R was autophosphorylated by yeast C to a level of 0.8 mol of phosphate/mol of R monomer.
T16 1585-1757 Sentence denotes By these criteria, the R subunit produced in E. coli was structurally and functionally identical to the natural yeast R subunit and similar to mammalian type II R subunits.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 14-30 OrganismTaxon denotes Escherichia coli 562
T2 107-131 OrganismTaxon denotes Saccharomyces cerevisiae 4932
T3 243-267 OrganismTaxon denotes Saccharomyces cerevisiae 4932
T4 285-301 OrganismTaxon denotes Escherichia coli 562
T5 353-360 OrganismTaxon denotes E. coli 562
T6 783-790 OrganismTaxon denotes E. coli 562
T7 1040-1047 OrganismTaxon denotes E. coli 562
T8 1630-1637 OrganismTaxon denotes E. coli 562