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sentences

Id Subject Object Predicate Lexical cue
T1 0-95 Sentence denotes Purification and characterization of a myosin heavy chain kinase from Dictyostelium discoideum.
T2 96-199 Sentence denotes A Dictyostelium discoideum myosin heavy chain kinase has been purified 14,000-fold to near homogeneity.
T3 200-380 Sentence denotes The enzyme has a Mr = 130,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and greater than 700,000 as determined by gel filtration on Bio-Gel A-1.5m.
T4 381-502 Sentence denotes The enzyme has a specific activity of 1 mumol/min X mg when assayed at a Dictyostelium myosin concentration of 0.3 mg/ml.
T5 503-627 Sentence denotes A maximum of 2 mol of phosphate/mol of myosin is incorporated by the kinase, and the phosphorylated amino acid is threonine.
T6 628-715 Sentence denotes Phosphate is incorporated only into the myosin heavy chains, not into the light chains.
T7 716-842 Sentence denotes The actin-activated Mg2+-ATPase of Dictyostelium myosin is inhibited 70-80% following maximal phosphorylation with the kinase.
T8 843-940 Sentence denotes The myosin heavy chain kinase requires 1-2 mM Mg2+ for activity and is most active at pH 7.0-7.5.
T9 941-1063 Sentence denotes The activity of the enzyme is not significantly altered by the presence of Ca2+, Ca2+ and calmodulin, EGTA, cAMP, or cGMP.
T10 1064-1191 Sentence denotes When incubated with Mg2+ and ATP, phosphate is incorporated into the myosin heavy chain kinase, perhaps by autophosphorylation.
T1 0-95 Sentence denotes Purification and characterization of a myosin heavy chain kinase from Dictyostelium discoideum.
T2 96-199 Sentence denotes A Dictyostelium discoideum myosin heavy chain kinase has been purified 14,000-fold to near homogeneity.
T3 200-380 Sentence denotes The enzyme has a Mr = 130,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and greater than 700,000 as determined by gel filtration on Bio-Gel A-1.5m.
T4 381-502 Sentence denotes The enzyme has a specific activity of 1 mumol/min X mg when assayed at a Dictyostelium myosin concentration of 0.3 mg/ml.
T5 503-627 Sentence denotes A maximum of 2 mol of phosphate/mol of myosin is incorporated by the kinase, and the phosphorylated amino acid is threonine.
T6 628-715 Sentence denotes Phosphate is incorporated only into the myosin heavy chains, not into the light chains.
T7 716-842 Sentence denotes The actin-activated Mg2+-ATPase of Dictyostelium myosin is inhibited 70-80% following maximal phosphorylation with the kinase.
T8 843-940 Sentence denotes The myosin heavy chain kinase requires 1-2 mM Mg2+ for activity and is most active at pH 7.0-7.5.
T9 941-1063 Sentence denotes The activity of the enzyme is not significantly altered by the presence of Ca2+, Ca2+ and calmodulin, EGTA, cAMP, or cGMP.
T10 1064-1191 Sentence denotes When incubated with Mg2+ and ATP, phosphate is incorporated into the myosin heavy chain kinase, perhaps by autophosphorylation.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 70-94 OrganismTaxon denotes Dictyostelium discoideum 44689
T2 98-122 OrganismTaxon denotes Dictyostelium discoideum 44689
T3 454-467 OrganismTaxon denotes Dictyostelium 5782
T4 751-764 OrganismTaxon denotes Dictyostelium 5782