PubMed:30234965
Annnotations
GlyCosmos15-Species-PubTator
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| 27 | 1017-1040 | Species | denotes | Clostridium perfringens | 1502 |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 424-431 | Glycan | denotes | lactose | |
| T2 | 435-442 | Glycan | denotes | lactose | |
| T3 | 517-524 | Glycan | denotes | lactose | |
| T4 | 629-636 | Glycan | denotes | lactose | |
| T5 | 893-900 | Glycan | denotes | lactose | |
| T6 | 1072-1079 | Glycan | denotes | lactose | |
| T7 | 1306-1313 | Glycan | denotes | lactose | |
| T8 | 1388-1395 | Glycan | denotes | lactose | |
| T9 | 1488-1495 | Glycan | denotes | lactose | |
| T10 | 1693-1700 | Glycan | denotes | lactose | |
| T11 | 1798-1805 | Glycan | denotes | lactose |
HP-phenotype
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 0-16 | Phenotype | denotes | Highly Sensitive | HP:0041092 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 424-431 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T2 | 435-442 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T3 | 517-524 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T4 | 629-636 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T5 | 893-900 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T6 | 1072-1079 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T7 | 1306-1313 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T8 | 1388-1395 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T9 | 1488-1495 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T10 | 1693-1700 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
| T11 | 1798-1805 | Glycan | denotes | lactose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE |
GlyCosmos15-HP
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 0-16 | Phenotype | denotes | Highly Sensitive | HP:0041092 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 451-455 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
| T2 | 1624-1628 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
| T3 | 1720-1724 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
| T4 | 1851-1854 | Body_part | denotes | fat | http://purl.obolibrary.org/obo/UBERON_0001013 |
GlyCosmos15-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 1017-1040 | OrganismTaxon | denotes | Clostridium perfringens | 1502 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-166 | Sentence | denotes | Highly Sensitive and Selective Biosensor for a Disaccharide Based on an AraC-Like Transcriptional Regulator Transduced with Bioluminescence Resonance Energy Transfer. |
| T2 | 167-308 | Sentence | denotes | Sensitive and selective quantification of individual sugars in complex media is technically challenging and usually requires HPLC separation. |
| T3 | 309-388 | Sentence | denotes | Accurate measurement without the need for separation would be highly desirable. |
| T4 | 389-637 | Sentence | denotes | The measurement of trace levels of lactose in lactose-reduced milk exemplifies the problem, with the added challenge that trace lactose must be measured in the presence of ≈140 mM glucose and galactose, the products of lactase digestion of lactose. |
| T5 | 638-821 | Sentence | denotes | Biosensing is an alternative to HPLC, but current biosensing methods, based on coupled-enzyme assays, tend to have poor sensitivity and complex biochemistry and can be time-consuming. |
| T6 | 822-964 | Sentence | denotes | We explored a fundamentally different approach, based on identifying a lactose-specific binding protein compatible with photonic transduction. |
| T7 | 965-1202 | Sentence | denotes | We identified the BgaR transcriptional regulator of Clostridium perfringens, which is highly selective for lactose, as a suitable ligand binding domain and combined it with a bioluminescence energy resonance transfer transduction system. |
| T8 | 1203-1314 | Sentence | denotes | This BRET-based biosensor showed a 27% decrease in the BRET ratio in the presence of saturating (1 mM) lactose. |
| T9 | 1315-1441 | Sentence | denotes | Using a 5 min assay, the half maximal effective concentration (EC50) for lactose in phosphate-buffered saline (PBS) was 12 μM. |
| T10 | 1442-1525 | Sentence | denotes | The biosensor was 200 times more sensitive to lactose than to glucose or galactose. |
| T11 | 1526-1629 | Sentence | denotes | Sensitivity and selectivity were not significantly affected by the presence of 10% (v/v) dialyzed milk. |
| T12 | 1630-1882 | Sentence | denotes | The biosensor is suitable for direct determination of residual lactose in lactase-treated milk, with a limit of detection of 0.2 μM, 100 times below the most stringent lactose-free standard and without the need to remove fat or protein from the sample. |
GlyCosmos15-FMA
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 451-455 | Body_part | denotes | milk | FMA:62100 |
| T2 | 1624-1628 | Body_part | denotes | milk | FMA:62100 |
| T3 | 1720-1724 | Body_part | denotes | milk | FMA:62100 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 1017-1040 | OrganismTaxon | denotes | Clostridium perfringens | 1502 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 451-455 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
| T2 | 1624-1628 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
| T3 | 1720-1724 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
| T4 | 1851-1854 | Body_part | denotes | fat | http://purl.obolibrary.org/obo/UBERON_0001013 |