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PubMed:30234965 JSONTXT

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HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 0-16 Phenotype denotes Highly Sensitive HP:0041092

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 424-431 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T2 435-442 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T3 517-524 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T4 629-636 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T5 893-900 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T6 1072-1079 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T7 1306-1313 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T8 1388-1395 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T9 1488-1495 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T10 1693-1700 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T11 1798-1805 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

GlyCosmos15-HP

Id Subject Object Predicate Lexical cue hp_id
T1 0-16 Phenotype denotes Highly Sensitive HP:0041092

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 451-455 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913
T2 1624-1628 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913
T3 1720-1724 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913
T4 1851-1854 Body_part denotes fat http://purl.obolibrary.org/obo/UBERON_0001013

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1017-1040 OrganismTaxon denotes Clostridium perfringens 1502

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-166 Sentence denotes Highly Sensitive and Selective Biosensor for a Disaccharide Based on an AraC-Like Transcriptional Regulator Transduced with Bioluminescence Resonance Energy Transfer.
T2 167-308 Sentence denotes Sensitive and selective quantification of individual sugars in complex media is technically challenging and usually requires HPLC separation.
T3 309-388 Sentence denotes Accurate measurement without the need for separation would be highly desirable.
T4 389-637 Sentence denotes The measurement of trace levels of lactose in lactose-reduced milk exemplifies the problem, with the added challenge that trace lactose must be measured in the presence of ≈140 mM glucose and galactose, the products of lactase digestion of lactose.
T5 638-821 Sentence denotes Biosensing is an alternative to HPLC, but current biosensing methods, based on coupled-enzyme assays, tend to have poor sensitivity and complex biochemistry and can be time-consuming.
T6 822-964 Sentence denotes We explored a fundamentally different approach, based on identifying a lactose-specific binding protein compatible with photonic transduction.
T7 965-1202 Sentence denotes We identified the BgaR transcriptional regulator of Clostridium perfringens, which is highly selective for lactose, as a suitable ligand binding domain and combined it with a bioluminescence energy resonance transfer transduction system.
T8 1203-1314 Sentence denotes This BRET-based biosensor showed a 27% decrease in the BRET ratio in the presence of saturating (1 mM) lactose.
T9 1315-1441 Sentence denotes Using a 5 min assay, the half maximal effective concentration (EC50) for lactose in phosphate-buffered saline (PBS) was 12 μM.
T10 1442-1525 Sentence denotes The biosensor was 200 times more sensitive to lactose than to glucose or galactose.
T11 1526-1629 Sentence denotes Sensitivity and selectivity were not significantly affected by the presence of 10% (v/v) dialyzed milk.
T12 1630-1882 Sentence denotes The biosensor is suitable for direct determination of residual lactose in lactase-treated milk, with a limit of detection of 0.2 μM, 100 times below the most stringent lactose-free standard and without the need to remove fat or protein from the sample.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 424-431 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T2 435-442 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T3 517-524 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T4 629-636 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T5 893-900 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T6 1072-1079 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T7 1306-1313 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T8 1388-1395 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T9 1488-1495 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T10 1693-1700 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T11 1798-1805 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1017-1040 OrganismTaxon denotes Clostridium perfringens 1502

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 451-455 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913
T2 1624-1628 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913
T3 1720-1724 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913
T4 1851-1854 Body_part denotes fat http://purl.obolibrary.org/obo/UBERON_0001013