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NGLY1-deficiency

Id Subject Object Predicate Lexical cue
PD-NGLY1-deficiency-B_T1 1416-1422 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T2 1982-1988 chem:24139 denotes GlcNAc
T1 1416-1422 chem:24139 denotes GlcNAc
T2 1982-1988 chem:24139 denotes GlcNAc

ngly1-sample5

Id Subject Object Predicate Lexical cue
T1 1416-1422 chem:24139 denotes GlcNAc
T2 1982-1988 chem:24139 denotes GlcNAc

Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-144 Sentence denotes Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.
T2 145-156 Sentence denotes Background:
T3 157-407 Sentence denotes Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.
T4 408-587 Sentence denotes Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.
T5 588-596 Sentence denotes Methods:
T6 597-821 Sentence denotes Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.
T7 822-830 Sentence denotes Results:
T8 831-991 Sentence denotes We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.
T9 992-1141 Sentence denotes The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.
T10 1142-1314 Sentence denotes A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.
T11 1315-1546 Sentence denotes MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.
T12 1547-1738 Sentence denotes The correlations between the BACH2 promoter methylation and IgG glycans were less obvious, since BACH2 is not a glycosyltransferase and therefore may affect IgG glycosylation only indirectly.
T13 1739-1751 Sentence denotes Conclusions:
T14 1752-2027 Sentence denotes Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.
T15 2028-2327 Sentence denotes Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.
T1 0-144 Sentence denotes Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease.
T2 145-156 Sentence denotes Background:
T3 157-407 Sentence denotes Many genome- and epigenome-wide association studies (GWAS and EWAS) and studies of promoter methylation of candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic and epigenetic changes and IBD.
T4 408-587 Sentence denotes Independent GWA studies have identified genetic variants in the BACH2, IL6ST, LAMB1, IKZF1, and MGAT3 loci to be associated with both IBD and immunoglobulin G (IgG) glycosylation.
T5 588-596 Sentence denotes Methods:
T6 597-821 Sentence denotes Using bisulfite pyrosequencing, we analyzed CpG methylation in promoter regions of these five genes from peripheral blood of several hundred IBD patients and healthy controls (HCs) from two independent cohorts, respectively.
T7 822-830 Sentence denotes Results:
T8 831-991 Sentence denotes We found significant differences in the methylation levels in the MGAT3 and BACH2 genes between both Crohn's disease and ulcerative colitis when compared to HC.
T9 992-1141 Sentence denotes The same pattern of methylation changes was identified for both genes in CD19+ B cells isolated from the whole blood of a subset of the IBD patients.
T10 1142-1314 Sentence denotes A correlation analysis was performed between the MGAT3 and BACH2 promoter methylation and individual IgG glycans, measured in the same individuals of the two large cohorts.
T11 1315-1546 Sentence denotes MGAT3 promoter methylation correlated significantly with galactosylation, sialylation, and bisecting GlcNAc on IgG of the same patients, suggesting that activity of the GnT-III enzyme, encoded by this gene, might be altered in IBD.
T12 1547-1738 Sentence denotes The correlations between the BACH2 promoter methylation and IgG glycans were less obvious, since BACH2 is not a glycosyltransferase and therefore may affect IgG glycosylation only indirectly.
T13 1739-1751 Sentence denotes Conclusions:
T14 1752-2027 Sentence denotes Our results suggest that epigenetic deregulation of key glycosylation genes might lead to an increase in pro-inflammatory properties of IgG in IBD through a decrease in galactosylation and sialylation and an increase of bisecting GlcNAc on digalactosylated glycan structures.
T15 2028-2327 Sentence denotes Finally, we showed that CpG methylation in the promoter of the MGAT3 gene is altered in CD3+ T cells isolated from inflamed mucosa of patients with ulcerative colitis from a third smaller cohort, for which biopsies were available, suggesting a functional role of this glyco-gene in IBD pathogenesis.