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Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-162 Sentence denotes Glial interleukin-1β upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats.
T2 163-174 Sentence denotes BACKGROUND:
T3 175-435 Sentence denotes The proinflammatory cytokine interleukin-1β (IL-1β) drives pain by inducing the expression of inflammatory mediators; however, its ability to regulate sodium channel 1.7 (Nav1.7), a key driver of temporomandibular joint (TMJ) hypernociception, remains unknown.
T4 436-503 Sentence denotes IL-1β induces cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2).
T5 504-587 Sentence denotes We previously showed that PGE2 upregulated trigeminal ganglionic Nav1.7 expression.
T6 588-670 Sentence denotes Satellite glial cells (SGCs) involve in inflammatory pain through glial cytokines.
T7 671-912 Sentence denotes Therefore, we explored here in the trigeminal ganglion (TG) whether IL-1β upregulated Nav1.7 expression and whether the IL-1β located in the SGCs upregulated Nav1.7 expression in the neurons contributing to TMJ inflammatory hypernociception.
T8 913-921 Sentence denotes METHODS:
T9 922-1336 Sentence denotes We treated rat TG explants with IL-1β with or without inhibitors, including NS398 for COX-2, PF-04418948 for EP2, and H89 and PKI-(6-22)-amide for protein kinase A (PKA), or with adenylate cyclase agonist forskolin, and used real-time PCR, Western blot, and immunohistofluorescence to determine the expressions or locations of Nav1.7, COX-2, cAMP response element-binding protein (CREB) phosphorylation, and IL-1β.
T10 1337-1422 Sentence denotes We used chromatin immunoprecipitation to examine CREB binding to the Nav1.7 promoter.
T11 1423-1669 Sentence denotes Finally, we microinjected IL-1β into the TGs or injected complete Freund's adjuvant into TMJs with or without previous microinjection of fluorocitrate, an inhibitor of SGCs activation, into the TGs, and evaluated nociception and gene expressions.
T12 1670-1781 Sentence denotes Differences between groups were examined by one-way analysis of variance (ANOVA) or independent samples t test.
T13 1782-2025 Sentence denotes RESULTS: IL-1β upregulated Nav1.7 mRNA and protein expressions in the TG explants, whereas NS398, PF-04418948, H89, or PKI-(6-22)-amide could all block this upregulation, and forskolin could also upregulate Nav1.7 mRNA and protein expressions.
T14 2026-2077 Sentence denotes IL-1β enhanced CREB binding to the Nav1.7 promoter.
T15 2078-2266 Sentence denotes Microinjection of IL-1β into the TGs or TMJ inflammation both induced hypernociception of TMJ region and correspondingly upregulated COX-2, phospho-CREB, and Nav1.7 expressions in the TGs.
T16 2267-2538 Sentence denotes Moreover, microinjection of fluorocitrate into the TGs completely blocked TMJ inflammation-induced activation of SGCs and the upregulation of IL-1β and COX-2 in the SGCs, and phospho-CREB and Nav1.7 in the neurons and alleviated inflammation-induced TMJ hypernociception.
T17 2539-2551 Sentence denotes CONCLUSIONS:
T18 2552-2771 Sentence denotes Glial IL-1β upregulated neuronal Nav1.7 expression via the crosstalk between signaling pathways of the glial IL-1β/COX-2/PGE2 and the neuronal EP2/PKA/CREB/Nav1.7 in TG contributing to TMJ inflammatory hypernociception.
T1 0-162 Sentence denotes Glial interleukin-1β upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats.
T2 163-174 Sentence denotes BACKGROUND:
T3 175-435 Sentence denotes The proinflammatory cytokine interleukin-1β (IL-1β) drives pain by inducing the expression of inflammatory mediators; however, its ability to regulate sodium channel 1.7 (Nav1.7), a key driver of temporomandibular joint (TMJ) hypernociception, remains unknown.
T4 436-503 Sentence denotes IL-1β induces cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2).
T5 504-587 Sentence denotes We previously showed that PGE2 upregulated trigeminal ganglionic Nav1.7 expression.
T6 588-670 Sentence denotes Satellite glial cells (SGCs) involve in inflammatory pain through glial cytokines.
T7 671-912 Sentence denotes Therefore, we explored here in the trigeminal ganglion (TG) whether IL-1β upregulated Nav1.7 expression and whether the IL-1β located in the SGCs upregulated Nav1.7 expression in the neurons contributing to TMJ inflammatory hypernociception.
T8 913-921 Sentence denotes METHODS:
T9 922-1336 Sentence denotes We treated rat TG explants with IL-1β with or without inhibitors, including NS398 for COX-2, PF-04418948 for EP2, and H89 and PKI-(6-22)-amide for protein kinase A (PKA), or with adenylate cyclase agonist forskolin, and used real-time PCR, Western blot, and immunohistofluorescence to determine the expressions or locations of Nav1.7, COX-2, cAMP response element-binding protein (CREB) phosphorylation, and IL-1β.
T10 1337-1422 Sentence denotes We used chromatin immunoprecipitation to examine CREB binding to the Nav1.7 promoter.
T11 1423-1669 Sentence denotes Finally, we microinjected IL-1β into the TGs or injected complete Freund's adjuvant into TMJs with or without previous microinjection of fluorocitrate, an inhibitor of SGCs activation, into the TGs, and evaluated nociception and gene expressions.
T12 1670-1781 Sentence denotes Differences between groups were examined by one-way analysis of variance (ANOVA) or independent samples t test.
T13 1782-2025 Sentence denotes RESULTS: IL-1β upregulated Nav1.7 mRNA and protein expressions in the TG explants, whereas NS398, PF-04418948, H89, or PKI-(6-22)-amide could all block this upregulation, and forskolin could also upregulate Nav1.7 mRNA and protein expressions.
T14 2026-2077 Sentence denotes IL-1β enhanced CREB binding to the Nav1.7 promoter.
T15 2078-2266 Sentence denotes Microinjection of IL-1β into the TGs or TMJ inflammation both induced hypernociception of TMJ region and correspondingly upregulated COX-2, phospho-CREB, and Nav1.7 expressions in the TGs.
T16 2267-2538 Sentence denotes Moreover, microinjection of fluorocitrate into the TGs completely blocked TMJ inflammation-induced activation of SGCs and the upregulation of IL-1β and COX-2 in the SGCs, and phospho-CREB and Nav1.7 in the neurons and alleviated inflammation-induced TMJ hypernociception.
T17 2539-2551 Sentence denotes CONCLUSIONS:
T18 2552-2771 Sentence denotes Glial IL-1β upregulated neuronal Nav1.7 expression via the crosstalk between signaling pathways of the glial IL-1β/COX-2/PGE2 and the neuronal EP2/PKA/CREB/Nav1.7 in TG contributing to TMJ inflammatory hypernociception.