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PubMed:29362428 JSONTXT

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CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 70-74 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T3 382-386 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T5 1046-1050 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 805-808 Glycan denotes Lac https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW

GlyCosmos15-CL

Id Subject Object Predicate Lexical cue cl_id
T1 70-74 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T3 382-386 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T5 1046-1050 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-90 Sentence denotes Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts.
T2 91-251 Sentence denotes Alcohol oxidase I (AOX1) promoter is the most popular but strictly-regulated methanol inducible promoter for heterologous protein expression in Pichia pastoris.
T3 252-348 Sentence denotes In recent years, AOX1 promoter libraries have been developed with deletion or insertion methods.
T4 349-480 Sentence denotes The present research manipulated poly (dA:dT) tracts in this promoter to control promoter strength, which hadn't been tried before.
T5 481-554 Sentence denotes There were 34 variants derived from the native AOX1 promoter constructed.
T6 555-683 Sentence denotes And variants were integrated into the same genomic location and upstream of the same reporter gene porcine growth hormone (pGH).
T7 684-929 Sentence denotes To test the transferability of the results obtained from reporter gene pGH, the variants were connected to reporter gene Lac Z. The resulted promoter library spanned an activity range between 0.25 and 3.5 fold of the wild-type promoter activity.
T8 930-1066 Sentence denotes In addition, activities of variants correlated with their predicted nucleosome architecture, which were directed by poly (dA:dT) tracts.
T9 1067-1238 Sentence denotes The cumulative sum of predicted nucleosome affinity across the region (-820 to -540) was related to promoters strength in single deletion variants on a proportional basis.
T10 1239-1459 Sentence denotes Overall, the research promotes understanding of the regulatory patterns for AOX1 promoter and suggested that varying promoter expression of engineering nucleosome architecture was also a feasible approach in P. pastoris.

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 38-53 OrganismTaxon denotes Pichia pastoris 4922
T2 235-250 OrganismTaxon denotes Pichia pastoris 4922

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 998-1008 Body_part denotes nucleosome FMA:67528
T2 1099-1109 Body_part denotes nucleosome FMA:67528
T3 1391-1401 Body_part denotes nucleosome FMA:67528

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 805-808 Glycan denotes Lac https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 38-53 OrganismTaxon denotes Pichia pastoris 4922
T2 235-250 OrganismTaxon denotes Pichia pastoris 4922