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Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-114 Sentence denotes Functional hepatocyte clusters on bioactive blend silk matrices towards generating bioartificial liver constructs.
T2 115-327 Sentence denotes The creation of in vitro functional hepatic tissue simulating micro-environmental niche of native liver is a keen area of research due to its demand in bioartificial liver (BAL) and cell-based tissue engineering.
T3 328-594 Sentence denotes Here, we investigated the potential of novel blend (BA) silk scaffold fabricated by blending mulberry (Bombyx mori, BM) silk fibroin with cell adhesion motif (RGD) rich non-mulberry (Antheraea assamensis, AA) silk fibroin, in generating a functional liver construct.
T4 595-800 Sentence denotes Three-dimensional (3D) porous silk scaffolds (BM, AA and BA) were physico-chemically characterized and functionally evaluated using human hepatocarcinoma cells (HepG2) and primary neonatal rat hepatocytes.
T5 801-943 Sentence denotes The growth and distribution of hepatocytes within the scaffolds were tracked by FESEM, alamar blue proliferation assay and live/dead staining.
T6 944-1085 Sentence denotes Hemocompatible BA scaffolds supported the formation of high density hepatocyte clusters, facilitating cell-matrix and cell-cell interactions.
T7 1086-1263 Sentence denotes Blend scaffolds evinced enhanced liver-specific functions of cultured hepatocytes in terms of albumin synthesis, urea synthesis and cytochrome P450 enzyme activity over 21 days.
T8 1264-1367 Sentence denotes Subcutaneous implantation of scaffolds demonstrated minimal macrophage infiltration in blend scaffolds.
T9 1368-1559 Sentence denotes These findings substantiate that the integral property of blend (BA) scaffold offers a befitting environment by influencing spheroidal growth of hepatocytes with enhanced biological activity.
T10 1560-1735 Sentence denotes Collectively, the present study provides a new 3D bio-matrix niche for growing functional liver cells that would have future prospects in BAL as well as regenerative medicine.
T11 1736-1762 Sentence denotes STATEMENT OF SIGNIFICANCE:
T12 1763-1878 Sentence denotes An end stage liver disease called cirrhosis perturbs the self-healing ability and physiological functions of liver.
T13 1879-2097 Sentence denotes Due to the scarcity of healthy donors, a functional in vitro hepatic construct retaining the liver-specific functions is in great demand for its prospects in bioartificial liver (BAL) and cell-based tissue engineering.
T14 2098-2237 Sentence denotes Physicochemical attributes of a matrix influence the behavior of cultured hepatocytes in terms of attachment, morphology and functionality.
T15 2238-2365 Sentence denotes Mulberry and non-mulberry silk fibroin presents unique amino acid sequence with difference in hydrophobicity and crystallinity.
T16 2366-2585 Sentence denotes Considering this, the present study focuses on the development of a suitable three-dimensional (3D) bioactive matrix incorporating both mulberry silk fibroin and cell adhesion motif (RGD) rich non-mulberry silk fibroin.
T17 2586-2758 Sentence denotes Porous silk blend scaffolds facilitated the formation of hepatocyte clusters with enhanced liver-specific functions emphasizing both cell-cell and cell-matrix interactions.
T18 2759-2933 Sentence denotes Hemocompatibility and integral property of blend scaffolds offers a biological niche for seeding functional liver cells that would have future prospects in biohybrid devices.
T1 0-114 Sentence denotes Functional hepatocyte clusters on bioactive blend silk matrices towards generating bioartificial liver constructs.
T2 115-327 Sentence denotes The creation of in vitro functional hepatic tissue simulating micro-environmental niche of native liver is a keen area of research due to its demand in bioartificial liver (BAL) and cell-based tissue engineering.
T3 328-594 Sentence denotes Here, we investigated the potential of novel blend (BA) silk scaffold fabricated by blending mulberry (Bombyx mori, BM) silk fibroin with cell adhesion motif (RGD) rich non-mulberry (Antheraea assamensis, AA) silk fibroin, in generating a functional liver construct.
T4 595-800 Sentence denotes Three-dimensional (3D) porous silk scaffolds (BM, AA and BA) were physico-chemically characterized and functionally evaluated using human hepatocarcinoma cells (HepG2) and primary neonatal rat hepatocytes.
T5 801-943 Sentence denotes The growth and distribution of hepatocytes within the scaffolds were tracked by FESEM, alamar blue proliferation assay and live/dead staining.
T6 944-1085 Sentence denotes Hemocompatible BA scaffolds supported the formation of high density hepatocyte clusters, facilitating cell-matrix and cell-cell interactions.
T7 1086-1263 Sentence denotes Blend scaffolds evinced enhanced liver-specific functions of cultured hepatocytes in terms of albumin synthesis, urea synthesis and cytochrome P450 enzyme activity over 21 days.
T8 1264-1367 Sentence denotes Subcutaneous implantation of scaffolds demonstrated minimal macrophage infiltration in blend scaffolds.
T9 1368-1559 Sentence denotes These findings substantiate that the integral property of blend (BA) scaffold offers a befitting environment by influencing spheroidal growth of hepatocytes with enhanced biological activity.
T10 1560-1735 Sentence denotes Collectively, the present study provides a new 3D bio-matrix niche for growing functional liver cells that would have future prospects in BAL as well as regenerative medicine.
T11 1736-1762 Sentence denotes STATEMENT OF SIGNIFICANCE:
T12 1763-1878 Sentence denotes An end stage liver disease called cirrhosis perturbs the self-healing ability and physiological functions of liver.
T13 1879-2097 Sentence denotes Due to the scarcity of healthy donors, a functional in vitro hepatic construct retaining the liver-specific functions is in great demand for its prospects in bioartificial liver (BAL) and cell-based tissue engineering.
T14 2098-2237 Sentence denotes Physicochemical attributes of a matrix influence the behavior of cultured hepatocytes in terms of attachment, morphology and functionality.
T15 2238-2365 Sentence denotes Mulberry and non-mulberry silk fibroin presents unique amino acid sequence with difference in hydrophobicity and crystallinity.
T16 2366-2585 Sentence denotes Considering this, the present study focuses on the development of a suitable three-dimensional (3D) bioactive matrix incorporating both mulberry silk fibroin and cell adhesion motif (RGD) rich non-mulberry silk fibroin.
T17 2586-2758 Sentence denotes Porous silk blend scaffolds facilitated the formation of hepatocyte clusters with enhanced liver-specific functions emphasizing both cell-cell and cell-matrix interactions.
T18 2759-2933 Sentence denotes Hemocompatibility and integral property of blend scaffolds offers a biological niche for seeding functional liver cells that would have future prospects in biohybrid devices.

silkworm_phenotype

Id Subject Object Predicate Lexical cue
T10 1180-1187 BMO_00461 denotes albumin
T7 1797-1815 Disease:MESH:D005355 denotes cirrhosis perturbs
T6 1766-1789 Disease:MESH:D058625 denotes end stage liver disease
T5 1199-1203 Chemical:MESH:D014508 denotes urea
T4 784-787 Species:10116 denotes rat
T3 727-732 Species:9606 denotes human
T2 511-531 Species:91021 denotes Antheraea assamensis
T1 431-442 Species:7091 denotes Bombyx mori

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 115-1735 None denotes The creation of in vitro functional hepatic tissue simulating micro-environmental niche of native liver is a keen area of research due to its demand in bioartificial liver (BAL) and cell-based tissue engineering. Here, we investigated the potential of novel blend (BA) silk scaffold fabricated by blending mulberry (Bombyx mori, BM) silk fibroin with cell adhesion motif (RGD) rich non-mulberry (Antheraea assamensis, AA) silk fibroin, in generating a functional liver construct. Three-dimensional (3D) porous silk scaffolds (BM, AA and BA) were physico-chemically characterized and functionally evaluated using human hepatocarcinoma cells (HepG2) and primary neonatal rat hepatocytes. The growth and distribution of hepatocytes within the scaffolds were tracked by FESEM, alamar blue proliferation assay and live/dead staining. Hemocompatible BA scaffolds supported the formation of high density hepatocyte clusters, facilitating cell-matrix and cell-cell interactions. Blend scaffolds evinced enhanced liver-specific functions of cultured hepatocytes in terms of albumin synthesis, urea synthesis and cytochrome P450 enzyme activity over 21 days. Subcutaneous implantation of scaffolds demonstrated minimal macrophage infiltration in blend scaffolds. These findings substantiate that the integral property of blend (BA) scaffold offers a befitting environment by influencing spheroidal growth of hepatocytes with enhanced biological activity. Collectively, the present study provides a new 3D bio-matrix niche for growing functional liver cells that would have future prospects in BAL as well as regenerative medicine.
T2 1763-2933 STATEMENT OF SIGNIFICANCE denotes An end stage liver disease called cirrhosis perturbs the self-healing ability and physiological functions of liver. Due to the scarcity of healthy donors, a functional in vitro hepatic construct retaining the liver-specific functions is in great demand for its prospects in bioartificial liver (BAL) and cell-based tissue engineering. Physicochemical attributes of a matrix influence the behavior of cultured hepatocytes in terms of attachment, morphology and functionality. Mulberry and non-mulberry silk fibroin presents unique amino acid sequence with difference in hydrophobicity and crystallinity. Considering this, the present study focuses on the development of a suitable three-dimensional (3D) bioactive matrix incorporating both mulberry silk fibroin and cell adhesion motif (RGD) rich non-mulberry silk fibroin. Porous silk blend scaffolds facilitated the formation of hepatocyte clusters with enhanced liver-specific functions emphasizing both cell-cell and cell-matrix interactions. Hemocompatibility and integral property of blend scaffolds offers a biological niche for seeding functional liver cells that would have future prospects in biohybrid devices.

silkworm

Id Subject Object Predicate Lexical cue
T1 431-442 Species:7091 denotes Bombyx mori
T2 511-531 Species:91021 denotes Antheraea assamensis
T3 727-732 Species:9606 denotes human
T4 784-787 Species:10116 denotes rat
T5 1199-1203 Chemical:MESH:D014508 denotes urea
T6 1766-1789 Disease:MESH:D058625 denotes end stage liver disease
T7 1797-1815 Disease:MESH:D005355 denotes cirrhosis perturbs

silkwormbase

Id Subject Object Predicate Lexical cue
T1 431-442 Species:7091 denotes Bombyx mori
T2 511-531 Species:91021 denotes Antheraea assamensis
T3 727-732 Species:9606 denotes human
T4 784-787 Species:10116 denotes rat
T5 1199-1203 Chemical:MESH:D014508 denotes urea
T6 1766-1789 Disease:MESH:D058625 denotes end stage liver disease
T7 1797-1815 Disease:MESH:D005355 denotes cirrhosis perturbs
T10 1180-1187 BMO_00461 denotes albumin