PubMed:29203451 / 156-815 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/29203451","sourcedb":"PubMed","sourceid":"29203451","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/29203451","text":"Reporter cells expressing a chimeric receptor that activates a reporter can be used for screening ligand-mediated signal transduction. In this study, we used reporter cells harboring an NFAT/lacZ construct that express β-galactosidase when the chimeric receptor is stimulated. A colorimetric β-galactosidase substrate, chlorophenol-red β-d-galactopyranoside (CPRG), was used to detect enzymatic activity. Sub-optimal conditions have unfortunately extensively been reported with such reporter-based β-galactosidase assays. Here, we aimed to improve the CPRG-based colorimetric assay such that receptor ligands could be effectively screened with reporter cells.","tracks":[{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":0,"end":659},"obj":"BACKGROUND"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_Structured_Abstracts"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubMed_Structured_Abstracts","color":"#93ece4","default":true}]}]}}