PubMed:29203451 / 1158-1683 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T3","span":{"begin":0,"end":525},"obj":"RESULTS"}],"text":"An increased CPRG substrate concentration combined with a different detergent, Saponin, and an optimal wavelength recording markedly increased the sensitivity for the detection of β-galactosidase activity (≈4-fold increase). Moreover, the improved protocol resulted in increased linear time-dependent recording of enzymatic activity once cells had been lysed, and a more stable and reproducible assay to detect a ligand-stimulus with the reporter cells. The optimal time length of exposure to a stimulus was ligand-dependent."}