PubMed:29175660 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/29175660","sourcedb":"PubMed","sourceid":"29175660","text":"Chemo-enzymatic synthesis of the glucagon containing N-linked oligosaccharide and its characterization.\nThe chemo-enzymatic synthesis of an artificially N-glycosylated derivative of glucagon, a peptide hormone that regulates the blood sugar level, is described. We synthesized the glycosylated glucagon by chemical synthesis of an N-acetylglucosaminyl peptide and enzymatic transfer of an oligosaccharide using the transglycosylation activity of the glycosynthase-like mutant of Mucor hiemalis endo-β-N-acetylglucosaminidase (Endo-M) and sialo-oligosaccharide oxazoline as a donor substrate. The sialo-oligosaccharide-attached glucagon synthesized showed high resistance against protease degradation and stimulated the release of glucose from mouse hepatocytes when added to cells. 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