PubMed:29108021 JSON TXT

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GoldHamster

PubMed_ArguminSci

{"project":"PubMed_ArguminSci","denotations":[{"id":"T1","span":{"begin":132,"end":261},"obj":"DRI_Background"},{"id":"T2","span":{"begin":262,"end":459},"obj":"DRI_Background"},{"id":"T3","span":{"begin":460,"end":550},"obj":"DRI_Background"},{"id":"T4","span":{"begin":551,"end":693},"obj":"DRI_Outcome"},{"id":"T5","span":{"begin":694,"end":942},"obj":"DRI_Approach"},{"id":"T6","span":{"begin":943,"end":1134},"obj":"DRI_Outcome"},{"id":"T7","span":{"begin":1135,"end":1321},"obj":"DRI_Background"}],"text":"Hippo, TGF-β, and Src-MAPK pathways regulate transcription of the upd3 cytokine in Drosophila enterocytes upon bacterial infection.\nCytokine signaling is responsible for coordinating conserved epithelial regeneration and immune responses in the digestive tract. In the Drosophila midgut, Upd3 is a major cytokine, which is induced in enterocytes (EC) and enteroblasts (EB) upon oral infection, and initiates intestinal stem cell (ISC) dependent tissue repair. To date, the genetic network directing upd3 transcription remains largely uncharacterized. Here, we have identified the key infection-responsive enhancers of the upd3 gene and show that distinct enhancers respond to various stresses. Furthermore, through functional genetic screening, bioinformatic analyses and yeast one-hybrid screening, we determined that the transcription factors Scalloped (Sd), Mothers against dpp (Mad), and D-Fos are principal regulators of upd3 expression. Our study demonstrates that upd3 transcription in the gut is regulated by the activation of multiple pathways, including the Hippo, TGF-β/Dpp, and Src, as well as p38-dependent MAPK pathways. Thus, these essential pathways, which are known to control ISC proliferation cell-autonomously, are also activated in ECs to promote tissue turnover the regulation of upd3 transcription."}

Goldhamster2_Cellosaurus

{"project":"Goldhamster2_Cellosaurus","denotations":[{"id":"T1","span":{"begin":18,"end":21},"obj":"CVCL_6492|Transformed_cell_line|Mus musculus"},{"id":"T2","span":{"begin":296,"end":297},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T3","span":{"begin":347,"end":349},"obj":"CVCL_DG46|Spontaneously_immortalized_cell_line|Oncorhynchus tshawytscha"},{"id":"T4","span":{"begin":369,"end":371},"obj":"CVCL_WY97|Cancer_cell_line|Homo sapiens"},{"id":"T5","span":{"begin":557,"end":559},"obj":"CVCL_5M23|Cancer_cell_line|Mesocricetus auratus"},{"id":"T6","span":{"begin":778,"end":781},"obj":"CVCL_Z424|Spontaneously_immortalized_cell_line|Ostrinia nubilalis"},{"id":"T7","span":{"begin":800,"end":802},"obj":"CVCL_5M23|Cancer_cell_line|Mesocricetus auratus"},{"id":"T8","span":{"begin":856,"end":858},"obj":"CVCL_W902|Cancer_cell_line|Homo sapiens"},{"id":"T9","span":{"begin":1021,"end":1031},"obj":"CVCL_C410|Hybridoma|Mus musculus"},{"id":"T10","span":{"begin":1090,"end":1093},"obj":"CVCL_6492|Transformed_cell_line|Mus musculus"},{"id":"T11","span":{"begin":1240,"end":1249},"obj":"CVCL_C410|Hybridoma|Mus musculus"},{"id":"T12","span":{"begin":1253,"end":1256},"obj":"CVCL_Z893|Spontaneously_immortalized_cell_line|Drosophila melanogaster"}],"text":"Hippo, TGF-β, and Src-MAPK pathways regulate transcription of the upd3 cytokine in Drosophila enterocytes upon bacterial infection.\nCytokine signaling is responsible for coordinating conserved epithelial regeneration and immune responses in the digestive tract. In the Drosophila midgut, Upd3 is a major cytokine, which is induced in enterocytes (EC) and enteroblasts (EB) upon oral infection, and initiates intestinal stem cell (ISC) dependent tissue repair. To date, the genetic network directing upd3 transcription remains largely uncharacterized. Here, we have identified the key infection-responsive enhancers of the upd3 gene and show that distinct enhancers respond to various stresses. Furthermore, through functional genetic screening, bioinformatic analyses and yeast one-hybrid screening, we determined that the transcription factors Scalloped (Sd), Mothers against dpp (Mad), and D-Fos are principal regulators of upd3 expression. Our study demonstrates that upd3 transcription in the gut is regulated by the activation of multiple pathways, including the Hippo, TGF-β/Dpp, and Src, as well as p38-dependent MAPK pathways. Thus, these essential pathways, which are known to control ISC proliferation cell-autonomously, are also activated in ECs to promote tissue turnover the regulation of upd3 transcription."}

PubMed:29108021 JSONTXT

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GoldHamster

PubMed_ArguminSci

Goldhamster2_Cellosaurus

PubMed:29108021 JSON TXT