PubMed:29099398 JSONTXT

{"project":"Inflammaging","denotations":[{"id":"T1","span":{"begin":0,"end":119},"obj":"Sentence"},{"id":"T2","span":{"begin":120,"end":130},"obj":"Sentence"},{"id":"T3","span":{"begin":131,"end":278},"obj":"Sentence"},{"id":"T4","span":{"begin":279,"end":573},"obj":"Sentence"},{"id":"T5","span":{"begin":574,"end":585},"obj":"Sentence"},{"id":"T6","span":{"begin":586,"end":642},"obj":"Sentence"},{"id":"T7","span":{"begin":643,"end":745},"obj":"Sentence"},{"id":"T8","span":{"begin":746,"end":794},"obj":"Sentence"},{"id":"T9","span":{"begin":795,"end":900},"obj":"Sentence"},{"id":"T10","span":{"begin":901,"end":1036},"obj":"Sentence"},{"id":"T11","span":{"begin":1037,"end":1154},"obj":"Sentence"},{"id":"T12","span":{"begin":1155,"end":1255},"obj":"Sentence"},{"id":"T13","span":{"begin":1256,"end":1330},"obj":"Sentence"},{"id":"T14","span":{"begin":1331,"end":1339},"obj":"Sentence"},{"id":"T15","span":{"begin":1340,"end":1440},"obj":"Sentence"},{"id":"T16","span":{"begin":1441,"end":1517},"obj":"Sentence"},{"id":"T17","span":{"begin":1518,"end":1669},"obj":"Sentence"},{"id":"T18","span":{"begin":1670,"end":1730},"obj":"Sentence"},{"id":"T19","span":{"begin":1731,"end":1893},"obj":"Sentence"},{"id":"T20","span":{"begin":1894,"end":1905},"obj":"Sentence"},{"id":"T21","span":{"begin":1906,"end":2080},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":119},"obj":"Sentence"},{"id":"T2","span":{"begin":120,"end":130},"obj":"Sentence"},{"id":"T3","span":{"begin":131,"end":278},"obj":"Sentence"},{"id":"T4","span":{"begin":279,"end":573},"obj":"Sentence"},{"id":"T5","span":{"begin":574,"end":585},"obj":"Sentence"},{"id":"T6","span":{"begin":586,"end":642},"obj":"Sentence"},{"id":"T7","span":{"begin":643,"end":745},"obj":"Sentence"},{"id":"T8","span":{"begin":746,"end":794},"obj":"Sentence"},{"id":"T9","span":{"begin":795,"end":900},"obj":"Sentence"},{"id":"T10","span":{"begin":901,"end":1036},"obj":"Sentence"},{"id":"T11","span":{"begin":1037,"end":1154},"obj":"Sentence"},{"id":"T12","span":{"begin":1155,"end":1255},"obj":"Sentence"},{"id":"T13","span":{"begin":1256,"end":1330},"obj":"Sentence"},{"id":"T14","span":{"begin":1331,"end":1339},"obj":"Sentence"},{"id":"T15","span":{"begin":1340,"end":1440},"obj":"Sentence"},{"id":"T16","span":{"begin":1441,"end":1517},"obj":"Sentence"},{"id":"T17","span":{"begin":1518,"end":1669},"obj":"Sentence"},{"id":"T18","span":{"begin":1670,"end":1730},"obj":"Sentence"},{"id":"T19","span":{"begin":1731,"end":1893},"obj":"Sentence"},{"id":"T20","span":{"begin":1894,"end":1905},"obj":"Sentence"},{"id":"T21","span":{"begin":1906,"end":2080},"obj":"Sentence"}],"text":"Electric Field Based Dressing Disrupts Mixed-Species Bacterial Biofilm Infection and Restores Functional Wound Healing.\nOBJECTIVE: This study was designed to employ electroceutical principles, as an alternative to pharmacological intervention, to manage wound biofilm infection. Mechanism of action of a United States Food and Drug Administration-cleared wireless electroceutical dressing (WED) was tested in an established porcine chronic wound polymicrobial biofilm infection model involving inoculation with Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii 19606.\nBACKGROUND: Bacterial biofilms represent a major wound complication. Resistance of biofilm toward pharmacologic interventions calls for alternative therapeutic strategies. Weak electric field has anti-biofilm properties. We have previously reported the development of WED involving patterned deposition of Ag and Zn on fabric. When moistened, WED generates a weak electric field without any external power supply and can be used as any other disposable dressing.\nMETHODS: WED dressing was applied within 2 hours of wound infection to test its ability to prevent biofilm formation. Alternatively, WED was applied after 7 days of infection to study disruption of established biofilm. Wounds were treated with placebo dressing or WED twice a week for 56 days.\nRESULTS: Scanning electron microscopy demonstrated that WED prevented and disrupted wound biofilm aggregates. WED accelerated functional wound closure by restoring skin barrier function. WED blunted biofilm-induced expression of (1) P. aeruginosa quorum sensing mvfR (pqsR), rhlR and lasR genes, and (2) miR-9 and silencing of E-cadherin. E-cadherin is critically required for skin barrier function. Furthermore, WED rescued against biofilm-induced persistent inflammation by circumventing nuclear factor kappa B activation and its downstream cytokine responses.\nCONCLUSION: This is the first pre-clinical porcine mechanistic study to recognize the potential of electroceuticals as an effective platform technology to combat wound biofilm infection."}