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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 136-612 BACKGROUND denotes Leishmaniasis threatens poor areas population worldwide, requiring new drugs less prone to resistance development. Antimicrobial peptides with antileishmanial activity are considered among fulfilling alternatives, but not much is known about the mode of action of membrane-targeting peptides, considering promastigote and infected macrophage membranes. In a previous work, structural features of very active known peptides were prospected using molecular dynamics simulations.
T2 622-1043 METHODS denotes Combining sequences of these peptides, analogs were designed. The structure of analog DecP-11 was validated by NMR. In vitro bioassays determined the peptide cytotoxicity toward mammalian cells, IC50 values on promastigotes and amastigotes, and membranolytic activity compared to Decoralin, one of the parent peptides. With biophysical methods, the mechanism of interaction with membrane mimetic systems was investigated.
T3 1053-1742 RESULTS denotes The designed peptide exhibits potent cytolytic and membrane permeabilizing activities, and decreased antileishmanial activity compared to the parent peptide. Interactions with lipid bilayers mimicking those of promastigotes, infected macrophage and mammalian cells showed that these peptides strongly bind to vesicles with intense lytic activity at low concentrations. Additionally, circular dichroism and light scattering experiments showed changes in the secondary structure of peptides and in vesicle size, depending on vesicles compositions. Altogether they suggest that DecP-11 antileishmanial activity is impaired by the aggregation and that aminophospholipids are probably involved.
T4 1756-1929 CONCLUSIONS denotes DecP-11 potent cytolytic and membranolytic activities with lack of selectivity toward promastigote model membranes warrant further structural studies to improve selectivity.
T5 1952-2097 GENERAL SIGNIFICANCE denotes Strong interactions of peptides with aminophospholipids, abundant in parasite membranes, potentially lead to aggregated forms impairing activity.

Goldhamster2_Cellosaurus

Id Subject Object Predicate Lexical cue
T1 51-59 CVCL_C410|Hybridoma|Mus musculus denotes activity
T2 295-303 CVCL_C410|Hybridoma|Mus musculus denotes activity
T3 492-493 CVCL_6479|Finite_cell_line|Mus musculus denotes a
T4 537-543 CVCL_C410|Hybridoma|Mus musculus denotes active
T5 713-715 CVCL_5986|Cancer_cell_line|Homo sapiens denotes 11
T6 881-889 CVCL_C410|Hybridoma|Mus musculus denotes activity
T7 913-916 CVCL_Z424|Spontaneously_immortalized_cell_line|Ostrinia nubilalis denotes one
T8 1128-1138 CVCL_C410|Hybridoma|Mus musculus denotes activities
T9 1170-1178 CVCL_C410|Hybridoma|Mus musculus denotes activity
T10 1390-1398 CVCL_C410|Hybridoma|Mus musculus denotes activity
T11 1495-1502 CVCL_0238|Cancer_cell_line|Homo sapiens denotes changes
T12 1633-1635 CVCL_5986|Cancer_cell_line|Homo sapiens denotes 11
T13 1652-1660 CVCL_C410|Hybridoma|Mus musculus denotes activity
T14 1761-1763 CVCL_5986|Cancer_cell_line|Homo sapiens denotes 11
T15 1799-1809 CVCL_C410|Hybridoma|Mus musculus denotes activities
T16 2088-2096 CVCL_C410|Hybridoma|Mus musculus denotes activity

GoldHamster

Id Subject Object Predicate Lexical cue
T1 19-27 D010455 denotes peptides
T2 19-27 D010455 denotes peptides
T3 19-27 CHEBI:16670 denotes peptides
T4 136-149 D007896 denotes Leishmaniasis
T5 136-149 D007896 denotes Leishmaniasis
T6 207-212 CHEBI:23888 denotes drugs
T7 251-264 CHEBI:33281 denotes Antimicrobial
T8 251-273 B3EWR6 denotes Antimicrobial peptides
T9 251-273 O24006 denotes Antimicrobial peptides
T10 265-273 D010455 denotes peptides
T11 265-273 D010455 denotes peptides
T12 265-273 CHEBI:16670 denotes peptides
T14 400-408 GO:0016020 denotes membrane
T13 400-408 UBERON:0000094 denotes membrane
T15 400-408 UBERON:0000158 denotes membrane
T16 419-427 D010455 denotes peptides
T17 419-427 D010455 denotes peptides
T18 419-427 CHEBI:16670 denotes peptides
T19 467-477 CL:0000235 denotes macrophage
T20 550-558 D010455 denotes peptides
T21 550-558 D010455 denotes peptides
T22 550-558 CHEBI:16670 denotes peptides
T23 651-659 D010455 denotes peptides
T24 651-659 D010455 denotes peptides
T25 651-659 CHEBI:16670 denotes peptides
T26 708-712 Q9VNE2 denotes DecP
T27 708-712 PR:Q9FNG3 denotes DecP
T28 720-729 SO:0000789 denotes validated
T29 733-736 PR:Q24418 denotes NMR
T30 733-736 P23762 denotes NMR
T32 772-779 PR:000018264 denotes peptide
T34 772-779 PR:000018263 denotes peptide
T33 772-779 CHEBI:16670 denotes peptide
T31 772-779 SO:0001064 denotes peptide
T37 902-911 P85870 denotes Decoralin
T35 902-911 C000607750 denotes Decoralin
T36 902-911 C000607750 denotes Decoralin
T38 931-939 D010455 denotes peptides
T39 931-939 D010455 denotes peptides
T40 931-939 CHEBI:16670 denotes peptides
T41 1001-1009 UBERON:0000094 denotes membrane
T43 1001-1009 UBERON:0000158 denotes membrane
T42 1001-1009 GO:0016020 denotes membrane
T45 1066-1073 PR:000018264 denotes peptide
T47 1066-1073 PR:000018263 denotes peptide
T44 1066-1073 SO:0001064 denotes peptide
T46 1066-1073 CHEBI:16670 denotes peptide
T48 1104-1112 UBERON:0000094 denotes membrane
T50 1104-1112 UBERON:0000158 denotes membrane
T49 1104-1112 GO:0016020 denotes membrane
T52 1202-1209 PR:000018264 denotes peptide
T54 1202-1209 PR:000018263 denotes peptide
T51 1202-1209 SO:0001064 denotes peptide
T53 1202-1209 CHEBI:16670 denotes peptide
T56 1229-1243 D008051 denotes lipid bilayers
T57 1287-1297 CL:0000235 denotes macrophage
T58 1336-1344 D010455 denotes peptides
T59 1336-1344 D010455 denotes peptides
T60 1336-1344 CHEBI:16670 denotes peptides
T61 1436-1444 SO:0000988 denotes circular
T62 1510-1529 SO:0001078 denotes secondary structure
T63 1533-1541 D010455 denotes peptides
T64 1533-1541 D010455 denotes peptides
T65 1533-1541 CHEBI:16670 denotes peptides
T66 1549-1556 GO:0031982 denotes vesicle
T67 1628-1632 Q9VNE2 denotes DecP
T68 1628-1632 PR:Q9FNG3 denotes DecP
T69 1701-1719 CHEBI:60971 denotes aminophospholipids
T70 1756-1760 Q9VNE2 denotes DecP
T71 1756-1760 PR:Q9FNG3 denotes DecP
T72 1815-1819 PR:Q9V853 denotes lack
T73 1975-1983 D010455 denotes peptides
T74 1975-1983 D010455 denotes peptides
T75 1975-1983 CHEBI:16670 denotes peptides
T76 1989-2007 CHEBI:60971 denotes aminophospholipids
T77 2021-2029 D010271 denotes parasite