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PubMed:2876884 JSONTXT

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Oryza_sentences

Id Subject Object Predicate Lexical cue
T1 0-111 Sentence denotes Human growth hormone (GH)-releasing factor stimulates and somatostatin inhibits the release of rat GH variants.
T2 112-391 Sentence denotes Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) was used for the analysis of proteins secreted by male rat pituitary cells in monolayer culture in the presence of 10 nM human GH-releasing factor (hGRF) or 30 nM somatostatin (SRIF) or in the absence of these factors.
T3 392-511 Sentence denotes More than 300 medium proteins were reproducibly detected either by fluorographic autoradiography or by silver staining.
T4 512-669 Sentence denotes Immunoreactivity of each protein was examined after 2D PAGE followed by Western blotting and immunostaining with affinity-purified antirat GH (rGH) antibody.
T5 670-837 Sentence denotes While there was a cluster of immunoreactive spots in the GH dimer range (40,000-50,000 mol wt), at least 16 medium proteins of mol wt 22,000 or less were also stained.
T6 838-962 Sentence denotes Among these 16 proteins the release of 15 was stimulated and the release of 14 was inhibited by hGRF and SRIF, respectively.
T7 963-1176 Sentence denotes On the other hand, there were 3 proteins of approximate mol wt 16,000 whose secretion was regulated in a coordinate manner as rGH by the hypothalamic factors but which did not cross-react with anti-rGH antibodies.
T8 1177-1374 Sentence denotes The increase or decrease in the radioactivity of each protein spot obtained from media after pituitary cells were incubated with [35S]methionine and hypothalamic factors was analyzed statistically.
T9 1375-1504 Sentence denotes A pulse-chase study suggested that at least 7 of the hormonally regulated proteins, including rGH, were synthesized very rapidly.
T10 1505-1713 Sentence denotes Finally, the 2D PAGE analysis of cell-free translation products of messenger RNA derived from male rat anterior pituitaries revealed the presence of about 40 rGH-immunoreactive proteins which included pre-GH.
T11 1714-1803 Sentence denotes These data suggest that there are multiple forms of rGH-variants or rGH-related proteins.
T12 1804-1944 Sentence denotes The biological significance(s) of all the rGH immunoreactive proteins and of the GRF- and SRIF-regulated pituitary proteins remains unclear.
T13 1945-2058 Sentence denotes It is evident that a number of these proteins are synthesized and released rapidly by pituitary cells in culture.
T14 2059-2282 Sentence denotes Furthermore, the presence of multiple genes for these rGH-related proteins is suggested by the large family of immunoreactive gene products identified after cell-free translation of messenger RNA derived from the pituitary.