> top > docs > PubMed:28743761 > annotations

PubMed:28743761 JSONTXT

Annnotations TAB JSON ListView MergeView

PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T1 119-328 DRI_Challenge denotes he sequence polymorphisms underlying complex trait variation is a key goal of genetics research, since knowing the precise causative molecular events allows insight into the pathways governing trait variation.
T2 329-489 DRI_Background denotes Genetic analysis of complex traits in model systems regularly starts by constructing QTL maps, but generally fails to identify causative sequence polymorphisms.
T3 490-708 DRI_Approach denotes Previously we mapped a series of QTL contributing to resistance to nicotine in a Drosophila melanogaster multiparental mapping resource, and here use a battery of functional tests to resolve QTL to the molecular level.
T4 709-942 DRI_Background denotes One large-effect QTL resided over a cluster of UDP-glucuronosyltransferases, and quantitative complementation tests using deficiencies eliminating subsets of these detoxification genes revealed allelic variation impacting resistance.
T5 943-1122 DRI_Outcome denotes RNAseq showed that Ugt86Dd had significantly higher expression in genotypes that are more resistant to nicotine, and anterior midgut-specific RNAi of this gene reduced resistance.
T6 1123-1332 DRI_Approach denotes We discovered a segregating 22-bp frameshift deletion in Ugt86Dd, and accounting for the InDel during mapping largely eliminates the QTL, implying the event explains the bulk of the effect of the mapped locus.
T7 1333-1532 DRI_Background denotes CRISPR/Cas9 editing a relatively resistant genotype to generate lesions in Ugt86Dd that recapitulate the naturally-occurring putative loss-of-function allele leads to a large reduction in resistance.
T8 1533-1720 DRI_Outcome denotes Despite this major effect of the deletion, the allele appears to be very rare in wild-caught populations, and likely explains only a small fraction of the natural variation for the trait.
T9 1721-1856 DRI_Challenge denotes Nonetheless, this putatively causative coding InDel can be a launchpad for future mechanistic exploration of xenobiotic detoxification.

kaiyin_test

Id Subject Object Predicate Lexical cue
T2 0-31 Var denotes Naturally-Segregating Variation
T1 35-42 Gene denotes Ugt86Dd
T3 43-54 PosReg denotes Contributes
T4 58-104 MPA denotes Nicotine Resistance in Drosophila melanogaster
T5 1157-1176 Var denotes frameshift deletion
T6 1180-1187 Gene denotes Ugt86Dd
T7 1241-1251 NegReg denotes eliminates
T8 1256-1259 MPA denotes QTL
T10 1397-1404 Var denotes lesions
T11 1408-1415 Gene denotes Ugt86Dd
T9 1467-1483 NegReg denotes loss-of-function
T12 1508-1517 NegReg denotes reduction
T13 1521-1531 MPA denotes resistance
R1 T2 T3 CauseOf Naturally-Segregating Variation,Contributes
R10 T11 T10 ThemeOf Ugt86Dd,lesions
R2 T1 T2 ThemeOf Ugt86Dd,Naturally-Segregating Variation
R3 T4 T3 ThemeOf Nicotine Resistance in Drosophila melanogaster,Contributes
R4 T5 T7 CauseOf frameshift deletion,eliminates
R5 T8 T7 ThemeOf QTL,eliminates
R6 T6 T5 ThemeOf Ugt86Dd,frameshift deletion
R7 T10 T9 CauseOf lesions,loss-of-function
R8 T10 T12 CauseOf lesions,reduction
R9 T13 T12 ThemeOf resistance,reduction

name_no

Id Subject Object Predicate Lexical cue
T2 0-31 Var denotes Naturally-Segregating Variation
T1 35-42 Gene denotes Ugt86Dd
T3 43-54 PosReg denotes Contributes
T4 58-104 MPA denotes Nicotine Resistance in Drosophila melanogaster
T5 1157-1176 Var denotes frameshift deletion
T6 1180-1187 Gene denotes Ugt86Dd
T7 1241-1251 NegReg denotes eliminates
T8 1256-1259 MPA denotes QTL
T10 1397-1404 Var denotes lesions
T11 1408-1415 Gene denotes Ugt86Dd
T9 1467-1483 NegReg denotes loss-of-function
T12 1508-1517 NegReg denotes reduction
T13 1521-1531 MPA denotes resistance
R1 T2 T3 CauseOf Naturally-Segregating Variation,Contributes
R10 T11 T10 ThemeOf Ugt86Dd,lesions
R2 T1 T2 ThemeOf Ugt86Dd,Naturally-Segregating Variation
R3 T4 T3 ThemeOf Nicotine Resistance in Drosophila melanogaster,Contributes
R4 T5 T7 CauseOf frameshift deletion,eliminates
R5 T8 T7 ThemeOf QTL,eliminates
R6 T6 T5 ThemeOf Ugt86Dd,frameshift deletion
R7 T10 T9 CauseOf lesions,loss-of-function
R8 T10 T12 CauseOf lesions,reduction
R9 T13 T12 ThemeOf resistance,reduction