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PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T1 120-310 DRI_Background denotes Development of Salmon Cardiac Primary Cultures (SCPCs) from Atlantic salmon pre-hatch embryos and their application as in vitro model for cardiotropic viral infection research are described.
T2 311-480 DRI_Background denotes Producing SCPCs requires plating of trypsin dissociated embryos with subsequent targeted harvest from 24h up to 3 weeks, of relevant tissues after visual identification.
T3 481-592 DRI_Approach denotes SCPCs are then transferred individually to chambered wells for culture in isolation, with incubation at 15-22°.
T4 593-904 DRI_Background denotes SCPCs production efficiency was not influenced by embryo's origin (0.75/ farmed or wild embryo), but mildly influenced by embryonic developmental stage (0.3 decline between 380 and 445 accumulated thermal units), and strongly influenced by time of harvest post-plating (0.6 decline if harvested after 72 hours).
T5 905-1115 DRI_Approach denotes Beating rate was not significantly influenced by temperature (15-22°) or age (2-4 weeks), but was significantly lower on SCPCs originated from farmed embryos with a disease resistant genotype (F = 5.3, p<0.05).
T6 1116-1298 DRI_Background denotes Two distinct morphologies suggestive of an ex vivo embryonic heart and a de novo formation were observed sub-grossly, histologically, ultra-structurally and with confocal microscopy.
T7 1299-1387 DRI_Approach denotes Both types contained cells consistent with cardiomyocytes, endothelium, and fibroblasts.
T8 1388-1545 DRI_Background denotes Ageing of SCPCs in culture was observed with increased auto fluorescence in live imaging, and as myelin figures and cellular degeneration ultra-structurally.
T9 1546-1689 DRI_Outcome denotes The SCPCs model was challenged with cardiotropic viruses and both the viral load and the mx gene expression were measurable along time by qPCR.
T10 1690-1861 DRI_Outcome denotes In summary, SCPCs represent a step forward in salmon cardiac disease research as an in vitro model that partially incorporates the functional complexity of the fish heart.

Goldhamster2_Cellosaurus

Id Subject Object Predicate Lexical cue
T1 0-15 CVCL_6F68|Spontaneously_immortalized_cell_line|Salmo salar denotes Atlantic salmon
T2 42-44 CVCL_8754|Cancer_cell_line|Homo sapiens denotes An
T3 42-44 CVCL_H241|Cancer_cell_line|Homo sapiens denotes An
T4 180-195 CVCL_6F68|Spontaneously_immortalized_cell_line|Salmo salar denotes Atlantic salmon
T5 336-343 CVCL_B488|Transformed_cell_line|Homo sapiens denotes plating
T6 413-416 CVCL_8262|Cancer_cell_line|Homo sapiens denotes 24h
T7 585-590 CVCL_H759|Finite_cell_line|Homo sapiens denotes 15-22
T8 588-590 CVCL_WT45|Hybridoma|Mus musculus denotes 22
T9 766-769 CVCL_1047|Cancer_cell_line|Homo sapiens denotes 380
T10 833-837 CVCL_0047|Telomerase_immortalized_cell_line|Homo sapiens denotes time
T11 854-861 CVCL_B488|Transformed_cell_line|Homo sapiens denotes plating
T12 966-977 CVCL_WR61|Induced_pluripotent_stem_cell|Homo sapiens denotes (15-22°) or
T13 967-972 CVCL_H759|Finite_cell_line|Homo sapiens denotes 15-22
T14 970-972 CVCL_WT45|Hybridoma|Mus musculus denotes 22
T15 978-981 CVCL_Z433|Spontaneously_immortalized_cell_line|Anthonomus grandis denotes age
T16 978-981 CVCL_Z658|Spontaneously_immortalized_cell_line|Anticarsia gemmatalis denotes age
T17 1068-1069 CVCL_6479|Finite_cell_line|Mus musculus denotes a
T18 1156-1158 CVCL_8754|Cancer_cell_line|Homo sapiens denotes an
T19 1156-1158 CVCL_H241|Cancer_cell_line|Homo sapiens denotes an
T20 1183-1191 CVCL_1N35|Spontaneously_immortalized_cell_line|Cricetulus griseus denotes and a de
T21 1187-1191 CVCL_1N36|Spontaneously_immortalized_cell_line|Cricetulus griseus denotes a de
T22 1187-1188 CVCL_6479|Finite_cell_line|Mus musculus denotes a
T23 1635-1637 CVCL_KC00|Hybridoma|Mus musculus denotes mx
T24 1676-1680 CVCL_0047|Telomerase_immortalized_cell_line|Homo sapiens denotes time
T25 1718-1719 CVCL_6479|Finite_cell_line|Mus musculus denotes a
T26 1771-1773 CVCL_8754|Cancer_cell_line|Homo sapiens denotes an
T27 1771-1773 CVCL_H241|Cancer_cell_line|Homo sapiens denotes an

GoldHamster

Id Subject Object Predicate Lexical cue
T2 0-15 900031 denotes Atlantic salmon
T3 0-15 CVCL_6F68 denotes Atlantic salmon
T1 0-15 8030 denotes Atlantic salmon
T4 0-15 D020317 denotes Atlantic salmon
T5 106-118 GO:0009405 denotes pathogenesis
T6 135-141 D012474 denotes Salmon
T8 180-195 900031 denotes Atlantic salmon
T9 180-195 CVCL_6F68 denotes Atlantic salmon
T7 180-195 8030 denotes Atlantic salmon
T10 180-195 D020317 denotes Atlantic salmon
T13 196-199 SO:0002045 denotes pre
T12 196-199 PR:Q8LLD0 denotes pre
T14 196-199 UBERON:0001782 denotes pre
T11 196-199 GO:0003904 denotes pre
T15 271-286 GO:0016032 denotes viral infection
T16 271-286 D014777 denotes viral infection
T17 271-286 D014777 denotes viral infection
T20 347-354 Q59149 denotes trypsin
T22 347-354 P70059 denotes trypsin
T23 347-354 P23916 denotes trypsin
T24 347-354 P00764 denotes trypsin
T25 347-354 P81071 denotes trypsin
T26 347-354 P35051 denotes trypsin
T27 347-354 P51588 denotes trypsin
T28 347-354 P83348 denotes trypsin
T29 347-354 P24664 denotes trypsin
T30 347-354 P00775 denotes trypsin
T31 347-354 O97399 denotes trypsin
T32 347-354 P19799 denotes trypsin
T33 347-354 P35048 denotes trypsin
T34 347-354 P35050 denotes trypsin
T35 347-354 P00761 denotes trypsin
T37 347-354 P35034 denotes trypsin
T39 347-354 P35049 denotes trypsin
T40 347-354 PR:000027795 denotes trypsin
T38 347-354 10890 denotes trypsin
T21 347-354 CHEBI:9765 denotes trypsin
T36 347-354 D014357 denotes trypsin
T41 413-416 CVCL_8262 denotes 24h
T42 643-649 UBERON:0000922 denotes embryo
T43 681-687 UBERON:0000922 denotes embryo
T44 715-724 P02301 denotes embryonic
T45 715-724 PR:000029532 denotes embryonic
T46 725-744 UBERON:0000105 denotes developmental stage
T47 978-984 PR:P40529 denotes age (2
T48 1070-1077 D004194 denotes disease
T49 1070-1077 D004194 denotes disease
T50 1088-1096 SO:0001027 denotes genotype
T51 1167-1176 P02301 denotes embryonic
T52 1167-1176 PR:000029532 denotes embryonic
T53 1197-1206 GO:0009058 denotes formation
T54 1221-1224 PR:Q8RWZ1 denotes sub
T55 1221-1224 PR:Q9V877 denotes sub
T56 1358-1369 UBERON:0001986 denotes endothelium
T57 1388-1394 GO:0007568 denotes Ageing
T58 1485-1491 UBERON:0000345 denotes myelin
T59 1595-1602 D014780 denotes viruses
T60 1638-1642 SO:0000704 denotes gene
T61 1638-1653 GO:0010467 denotes gene expression
T62 1725-1732 SO:0001030 denotes forward
T63 1743-1758 D006331 denotes cardiac disease
T64 1743-1758 D006331 denotes cardiac disease
T67 1850-1854 PR:Q24533 denotes fish