PubMed:2866182 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2866182","sourcedb":"PubMed","sourceid":"2866182","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2866182","text":"gamma-Interferon increases expression of class III complement genes C2 and factor B in human monocytes and in murine fibroblasts transfected with human C2 and factor B genes.\ngamma-Interferon (IFN-gamma) is a well characterized lymphokine known to regulate many mononuclear phagocyte functions, including expression of class I and class II major histocompatibility complex genes. The second component of complement (C2) and factor B are major histocompatibility complex class III gene products synthesized in mononuclear phagocytes. Recombinant IFN-gamma increased the synthesis of C2 and factor B in primary cultures of human mononuclear phagocytes and in murine fibroblasts transfected with cosmid DNA bearing the human C2 and factor B genes. In both cell types the increases in C2 and factor B protein synthesis were detected at concentrations of IFN-gamma less than 1 unit/ml and the regulation of each was pretranslational. The IFN-gamma-induced increases in C2 and factor B mRNA did not require new protein synthesis. In primary cultures of human monocytes, the kinetics of induction of C2 and factor B synthesis differed, but in the transfected L-cells the kinetics were similar, suggesting differences in transduction of the IFN-gamma signal, transcriptional, and/or post-transcriptional events in the two cell types. The small size of the factor B 5' flanking region, which is bounded by the 3' terminus of the IFN-gamma-regulated C2 gene, provides a well defined region to probe the structural basis for IFN-gamma regulation of gene expression.","tracks":[{"project":"2015-BEL-Sample-2","denotations":[{"id":"BEL:20053632","span":{"begin":533,"end":743},"obj":"p(MGI:Ifng) increases p(MGI:C2)"},{"id":"BEL:20053640","span":{"begin":533,"end":743},"obj":"p(MGI:Ifng) increases 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